Validation of molecular markers for wheat breeding

2001 ◽  
Vol 52 (12) ◽  
pp. 1357 ◽  
Author(s):  
P. J. Sharp ◽  
S. Johnston ◽  
G. Brown ◽  
R. A. McIntosh ◽  
M. Pallotta ◽  
...  
Keyword(s):  
Plant Resistance ◽  
Stem Rust ◽  
Adult Plant Resistance ◽  
Wheat Breeding ◽  
Chromosome Segment ◽  
Adult Plant ◽  
Wheat Stem Rust ◽  
Aegilops Ventricosa ◽  
Flour Colour ◽  
Trait Locus

Five sets of markers were assessed for their usefulness in breeding, two linked to wheat stem rust gene Sr2, several markers linked to a chromosome segment conferring Yr17/Lr37/Sr38 resistance, two reported markers for the linked genes Lr35 andSr39, one for Lr28, and one linked to flour colour. The gene for Sr2 confers adult plant resistance to stem rust (Puccinia graminis f.sp. tritici) and was originally transferred to bread wheat from the tetraploid emmer (‘Yaroslav’) to the cultivars Hope and H-44. The gene is located on the short arm of chromosome 3B and confers a durable adult plant resistance to stem rust usually expressed only in the field. The chromosome segment carrying the Lr37, Sr38, Yr17 resistance genes is located on 2AS and was originally introduced into wheat through an Aegilops ventricosa Triticum persicum cross, followed by a cross to the cultivar Marne (VPM1). The flour colour quantitative trait locus was originally described in a Yarralinka Schomburg cross and is located on chromosome 7A. The primers as originally developed required optimisation for more routine use in a breeding program.

Molecular Characterization of Genomic Regions for Adult Plant Resistance to Stem Rust in a Spring Wheat Mapping Population

Plant Disease ◽  
2021 ◽  
Author(s):  
Yahya Rauf ◽  
Prabin Bajgain ◽  
Matthew Rouse ◽  
Khalil A Khanzada ◽  
Sridhar Bhavani ◽  
...  
Keyword(s):  
Spring Wheat ◽  
Plant Resistance ◽  
Stem Rust ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Durable Resistance ◽  
Stem Rust Resistance ◽  
Adult Plant ◽  
Wheat Stem Rust ◽  
Genomic Regions

Adult plant resistance (APR) to wheat stem rust has been one of the approaches for resistance breeding since the evolution of the Ug99 race group and other races. This study was conducted to dissect and understand the genetic basis of APR to stem rust in spring wheat line ‘Copio’. A total of 176 recombinant inbred lines (RIL) from the cross of susceptible parent ‘Apav’ with Copio were phenotyped for stem rust resistance in six environments. Composite interval mapping (CIM) using 762 Genotyping-by-Sequencing (GBS) markers, identified 16 genomic regions conferring stem rust resistance. Assays with gene-linked molecular markers revealed that Copio carried known APR genes Sr2 and Lr46/Yr29/Sr58 in addition to the 2NS/2AS translocation that harbors race-specific genes Sr38, Lr37 and Yr17. Three QTL were mapped on chromosomes 2B, two QTL on chromosomes 3A, 3B, and 6A each, and one QTL on each of chromosomes 2A, 1B, 2D, 4B, 5D, 6D and 7A. The QTL QSr.umn.5D is potentially a new resistance gene and contributed to quantitative resistance in Copio. The RILs with allelic combinations of Sr2, Sr38, and Sr58 had 27-39% less stem rust coefficient of infection in all field environments compared to RILs with none of these genes and this gene combination was most effective in the US environments. We conclude that Copio carries several genes that provide both race-specific and non-race-specific resistance to diverse races of stem rust fungus and can be used by breeding programs in pyramiding other effective genes to develop durable resistance in wheat.

Seedling resistances to rust diseases in international triticale germplasm

2010 ◽  
Vol 61 (12) ◽  
pp. 1036 ◽  
Author(s):  
J. Zhang ◽  
C. R. Wellings ◽  
R. A. McIntosh ◽  
R. F. Park
Keyword(s):  
Leaf Rust ◽  
Stripe Rust ◽  
Plant Resistance ◽  
Stem Rust ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Stem Rust Resistance ◽  
Adult Plant ◽  
Seedling Resistance ◽  
Rust Diseases

Seedling resistances to stem rust, leaf rust and stripe rust were evaluated in the 37th International Triticale Screening Nursery, distributed by the International Wheat and Maize Improvement Centre (CIMMYT) in 2005. In stem rust tests, 12 and 69 of a total of 81 entries were postulated to carry Sr27 and SrSatu, respectively. When compared with previous studies of CIMMYT triticale nurseries distributed from 1980 to 1986 and 1991 to 1993, the results suggest a lack of expansion in the diversity of stem rust resistance. A total of 62 of 64 entries were resistant to five leaf rust pathotypes. In stripe rust tests, ~93% of the lines were postulated to carry Yr9 alone or in combination with other genes. The absence of Lr26 in these entries indicated that Yr9 and Lr26 are not genetically associated in triticale. A high proportion of nursery entries (63%) were postulated to carry an uncharacterised gene, YrJackie. The 13 lines resistant to stripe rust and the 62 entries resistant to leaf rust represent potentially useful sources of seedling resistance in developing new triticale cultivars. Field rust tests are needed to verify if seedling susceptible entries also carry adult plant resistance.

Inheritance of resistance to stem rust in 'Bonza', 'Chris', 'FKN-II-50-17', 'MRFY', 'Thatcher', 'Marquillo', and 'Hope' wheats

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 269-276
Author(s):  
M. Padidam ◽  
D. R. Knott
Keyword(s):  
Triticum Aestivum ◽  
Plant Resistance ◽  
Stem Rust ◽  
Adult Plant Resistance ◽  
Major Gene ◽  
Triticum Aestivum L ◽  
Adult Plant ◽  
Puccinia Graminis ◽  
Seedling Resistance ◽  
Minor Genes

Resistance to stem rust (Puccinia graminis Pers. f. sp. tritici Eriks, and Henn.), particularly adult plant resisitance to race 15B-1, was studied in seven wheat (Triticum aestivum L.) cultivars or lines: 'Bonza', 'Chris', 'FKN-II-50-17', 'MRFY', 'Thatcher', 'Marquillo', and 'Hope'. Each of the seven was crossed with a susceptible parent and either F4- or F5-derived lines developed by single seed descent. All of the lines were tested with race 15B-1 in field nurseries. Lines derived from parents carrying seedling resistance to race 15B-1 were also tested as seedlings in the greenhouse with race 15B-1, and in some cases races 56, 29, and C65. The data indicated that 'Bonza' carries Sr6, probably Sr5, an unidentified gene giving resistance to race 56, two unidentified genes for resistance to race C65, and two minor genes that combine to produce intermediate adult plant resistance. 'Chris' carries Sr5, Sr7a, Sr8a, and Sr12. In addition, it may have three minor genes for adult plant resistance. 'FKN-II-50-17' carries Sr6 and may have four minor genes that combine to produce moderate adult plant resistance. 'MRFY', which is seedling susceptible to race 15B-1, carries Sr9b, possibly Sr5, plus an unidentified gene for resistance to C65. In addition, it appears to have one major gene for adult plant resistance plus two or more minor genes. 'Thatcher', 'Marquillo', and 'Hope' had only limited resistance to race 15B-1 in the field and no genetic analysis of their crosses was possible. The four parents that had good resistance to race 15B-1 in the field, 'Bonza', 'Chris', 'FKN-II-50-17', and 'MRFY', all carry minor genes for adult plant resistance that had little effect individually but produced moderate resistance when combined. The genes Sr5 and Sr9b, which have no effect on resistance to 15B-1 is seedlings, were found to significantly increase resistance in adult plants in the field.Key words: stem rust, Puccinia graminis tritici, wheat, Triticum aestivum, adult plant rust resistance.

QTL mapping of adult plant resistance to Ug99 stem rust in the spring wheat population RB07/MN06113-8

2015 ◽  
Vol 35 (8) ◽  
Author(s):  
Prabin Bajgain ◽  
Matthew N. Rouse ◽  
Sridhar Bhavani ◽  
James A. Anderson
Keyword(s):  
Qtl Mapping ◽  
Spring Wheat ◽  
Plant Resistance ◽  
Stem Rust ◽  
Adult Plant Resistance ◽  
Adult Plant ◽  
Wheat Population

GENES FOR STEM RUST RESISTANCE IN WHEAT VARIETIES HOPE AND H-44

1971 ◽  
Vol 13 (2) ◽  
pp. 186-188 ◽  
Author(s):  
D. R. Knott
Keyword(s):  
Plant Resistance ◽  
Stem Rust ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Recessive Gene ◽  
Stem Rust Resistance ◽  
Adult Plant ◽  
Wheat Varieties ◽  
Chromosome 3B

Tests were carried out to identify and locate the genes for resistance to races 15B-1L and 56 in Hope and H-44. The gene Sr1 which conditions resistance to race 56 was found to be either very closely linked or more probably allelic to Sr9. It is proposed that it be redesignated Sr9d. The gene Sr2 which conditions adult plant resistance to race 56 appears to be on chromosome 3B. The recessive gene conditioning resistance to race 15B-1L was identified as sr17 which is on chromosome 7B.

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