Electrochemical Detection of Short DNA Sequences Related to the Escherichia coli Pathogen Using a Zirconia-Modified Screen-Printed DNA Biosensor

2008 ◽  
Vol 61 (12) ◽  
pp. 962 ◽  
Author(s):  
Shao-Hua Zuo ◽  
Ling-Fan Zhang ◽  
Yan-Hui Zhao ◽  
Hui-Hui Yuan ◽  
Min-Bo Lan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Differential Pulse Voltammetry ◽  
Dna Sequences ◽  
Differential Pulse ◽  
Dna Biosensor ◽  
Phosphate Group ◽  
Complementary Sequence ◽  
Pulse Voltammetry ◽  
Screen Printed

A simple, disposable and inexpensive electrochemical DNA biosensor based on a zirconia (ZrO2) modified thin film screen-printed electrode (ZrO2/SPE) has been developed. Short DNA sequences (21 monomer units) from the Escherichia coli pathogen, modified with a phosphate group at the 5′ end, were attached to the surface of the electrode through the affinity of the phosphate group for zirconia, to produce an effective DNA probe (ssDNA/ZrO2/SPE). DNA immobilization and hybridization were characterized using differential pulse voltammetry by employing methylene blue as redox indicator. Target sequences hybridized with the probe resulted in a decrease of the reduction peak current of methylene blue intercalated into the probe. The response of a non-complementary sequence and a single base pair mismatch sequence were both clearly distinguished from that of a complementary sequence. The developed biosensor had a high selectivity and sensitivity towards hybridization detection (10–10 M complementary DNA detectable). Making use of screen-printed technology, the fabrication of the biosensors exhibited satisfactory reproducibility, investigated by cyclic voltammetry and differential pulse voltammetry. The relative standard deviation was found to be <3.0% for six bare SPEs and six ssDNA-modified SPEs (ssDNA/ZrO2/SPE) from a batch.

A signal-off aptasensor for the determination of Ochratoxin A by differential pulse voltammetry at a modified Au electrode using methylene blue as an electrochemical probe

2017 ◽  
Vol 9 (37) ◽  
pp. 5449-5454 ◽  
Author(s):  
Min Wei ◽  
Shuo Feng
Keyword(s):  
Methylene Blue ◽  
Differential Pulse Voltammetry ◽  
Ochratoxin A ◽  
Differential Pulse ◽  
Au Electrode ◽  
Electrochemical Probe ◽  
Pulse Voltammetry

A signal-off aptasensor for OTA detection at an apt/cDNA/AuNPs/cPC/NH2–AuE electrode using methylene blue as an electrochemical probe.

scholarly journals CUPRAC Voltammetric Determination of Antioxidant Capacity in Tea Samples by Using Screen-Printed Microelectrodes

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Alina Elena Trofin ◽  
Lucia Carmen Trincă ◽  
Elena Ungureanu ◽  
Adina Mirela Ariton
Keyword(s):  
Antioxidant Capacity ◽  
Differential Pulse Voltammetry ◽  
Low Cost ◽  
Differential Pulse ◽  
Food Samples ◽  
Open Circuit ◽  
Rapid Response ◽  
Detection Methods ◽  
Pulse Voltammetry ◽  
Screen Printed

Measurement of antioxidant capacity represents an analytical major challenge in terms of accuracy, efficiency, rapid response, or low cost of detection methods. Quantification of antioxidant capacity of food samples using disposable screen‐printed microelectrodes (SPMEs) was based on cyclic voltammetry versus open-circuit potential (CV vs OCP) and differential pulse voltammetry (DPV) as compared with spectrophotometric measurement of the CUPRAC reaction with 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox). The SPMEs are organic‐resistant electrodes and thus compatible with food samples and organic solvents used to dissolve trolox. A micropipette was used to release a drop of 50 μL sample on the spotted surface of the SPME sensor/working electrode that was time programmed to function according to the working protocol. The SPME response was linearly correlated with trolox content. This preliminary demonstration was focused on the analysis of tea infusions, due to the simplicity and reproducibility of the samples’ preparations involved. Analytical results of the antioxidant capacity (expressed as mol·L−1 trolox equivalents) of the tea samples showed a good agreement in the case of spectrophotometry and differential pulse voltammetry (R2 > 0.998). DPV with SPME based on CUPRAC reactions was proven to be a promising approach for the characterization of antioxidant capacity of tea samples with rapid response, cost-effectiveness, and simplicity of operation.

scholarly journals Electrochemical determination of tramadol using modified screen printed electrode

2021 ◽  
Author(s):  
Sayed ali Ahmadi ◽  
Sayed Zia Mohammadi ◽  
Maedeh Jafari ◽  
Peyman Mohammadzadeh Jahani ◽  
Raana Mashayekh
Keyword(s):  
Differential Pulse Voltammetry ◽  
Differential Pulse ◽  
Diagnostic Techniques ◽  
Electrochemical Determination ◽  
Screen Printed Electrode ◽  
Dynamic Activity ◽  
Multi Walled Carbon Nanotubes ◽  
Pulse Voltammetry ◽  
Walled Carbon Nanotubes ◽  
Screen Printed

The detection of tramadol using a screen printed electrode modified with La3+/ZnO nano-flowers and multi-walled carbon nanotubes (La3+/ZnO NFs-MWCNTs/SPE) is reported in this work. In order to examine tramadol electrochemical oxidation, the modified electrode was implemented with the utilization of differential pulse voltammetry, chronoamperometry and cyclic voltammetry as diagnostic techniques. The proposed electrode displays favorable electrocatalytic behavior concerning tramadol oxidation with an approximately 330 mV potential shift to a lesser positive potential. In the 0.5 to 800.0 μM range for tramadol, differential pulse voltammetry displays linear dynamic activity. Tramadol detection limit of 0.08 μM was derived within optimized testing conditions for this simple construction sensor. Lastly, this fabricated sensor was utilized with desirable results to determine tramadol in tramadol samples and urine samples.

Simultaneous Determination of Cr(III) and Cr(VI) by Differential Pulse Voltammetry Using Modified Screen-Printed Carbon Electrodes in Array Mode

2010 ◽  
Vol 22 (24) ◽  
pp. 2924-2930 ◽  
Author(s):  
Ana Calvo-Pérez ◽  
Olga Domínguez-Renedo ◽  
M. Asunción Alonso-Lomillo ◽  
M. Julia Arcos-Martínez
Keyword(s):  
Differential Pulse Voltammetry ◽  
Simultaneous Determination ◽  
Differential Pulse ◽  
Carbon Electrodes ◽  
Pulse Voltammetry ◽  
Screen Printed

Screen Printed Carbon Nano-Powder for Ascorbic Acid Determination with Differential Pulse Voltammetry

2014 ◽  
Vol 979 ◽  
pp. 378-381
Author(s):  
W. Wonsawat ◽  
K. Somjit ◽  
N. Wangnaitum ◽  
O. Chailapakul
Keyword(s):  
Ascorbic Acid ◽  
Differential Pulse Voltammetry ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Working Electrode ◽  
Limit Of Quantitation ◽  
Pulse Voltammetry ◽  
Ascorbic Acid Determination ◽  
Acid Determination ◽  
Screen Printed

Carbon nanopowder mixed with carbon ink was used as a working screen-printed electrode. This electrode consists of carbon nanopowder working electrode, carbon counter electrode, and silver/silver chloride reference electrode. The electrochemical behavior of ascorbic acid has been studied by cyclic voltammetry and differential pulse voltammetry with screen-printed carbon nanopowder electrode. The propose electrode will enhanced the sensitivity for antioxidance determination because increasing of the surface area of the working electrode. The voltammetric sensor offers several advantages to both the green chemistry and eco-friendly. The method was successfully employed with 2 microliter of the working solution dropped on the electrode surface. A linear concentration, limit of quantitation and limit of detection was sufficient for the ascorbic acid determination in the vitamin C tablet. The results obtained for the sample juices demonstrated that the powerful of the method.

Investigation of antimalarial drug pyrimethamine and its interaction with dsDNA by electrochemical and spectroscopic techniques

2015 ◽  
Vol 7 (10) ◽  
pp. 4159-4167 ◽  
Author(s):  
Abd-Elgawad Radi ◽  
Hossam M. Nassef ◽  
Mohamed I. Attallah
Keyword(s):  
Cyclic Voltammetry ◽  
Differential Pulse Voltammetry ◽  
Electrochemical Behavior ◽  
Antimalarial Drug ◽  
Differential Pulse ◽  
Spectroscopic Techniques ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Pulse Voltammetry ◽  
Screen Printed

The electrochemical behavior of the antimalarial drug pyrimethamine (PMT) was examined at a screen printed carbon electrode (SPCE) in different aqueous supporting electrolytes using cyclic voltammetry (CV) and differential pulse voltammetry (DPV).

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