scholarly journals An Oxidized Abasic Lesion as an Intramolecular Source of DNA Adducts

2011 ◽  
Vol 64 (4) ◽  
pp. 438 ◽  
Author(s):  
Lirui Guan ◽  
Marc M. Greenberg
Keyword(s):  
Dna Sequence ◽  
Dna Adducts ◽  
High Yields ◽  
Cis And Trans ◽  
Exocyclic Adducts ◽  
Nucleoside Adducts

5′-(2-Phosphoryl-1,4-dioxobutane) (DOB) is a lesion produced in DNA via a variety of damaging agents. The DOB lesion spontaneously generates cis- and trans-but-2-en-1,4-dial (1) via β-elimination. Cis- and trans-but-2-en-1,4-dial forms exocyclic adducts with nucleosides. We used chemically synthesized DNA containing tritiated DOB incorporated at defined sites to examine the reactivity of cis- and trans-but-2-en-1,4-dial. Although the local DNA sequence does not appear to influence the distribution of nucleoside adducts, we find that DOB generates relatively high yields of cis- and trans-but-2-en-1,4-dial nucleoside adducts that likely are promutagenic.

scholarly journals Biphasic removal of DNA adducts in a repetitive DNA sequence after dietary administration of 2-acetylaminofluorene.

1993 ◽  
Vol 99 ◽  
pp. 273-275 ◽  
Author(s):  
S J Culp ◽  
M C Poirier ◽  
F A Beland
Keyword(s):  
Dna Sequence ◽  
Repetitive Dna ◽  
Dna Adducts ◽  
Repetitive Dna Sequence

Design, synthesis, and DNA sequence selectivity of formaldehyde-mediated DNA-adducts of the novel N -(4-aminobutyl) acridine-4-carboxamide

2014 ◽  
Vol 24 (24) ◽  
pp. 5710-5715 ◽  
Author(s):  
Elizabeth A. Ankers ◽  
Benny J. Evison ◽  
Don R. Phillips ◽  
Robert T.C. Brownlee ◽  
Suzanne M. Cutts
Keyword(s):  
Dna Sequence ◽  
Dna Adducts ◽  
The Novel ◽  
Design Synthesis ◽  
Sequence Selectivity

Editing of pre-mRNAs can occur before cis- and trans-splicing in Petunia mitochondria

1991 ◽  
Vol 11 (8) ◽  
pp. 4274-4277
Author(s):  
C A Sutton ◽  
P L Conklin ◽  
K D Pruitt ◽  
M R Hanson
Keyword(s):  
Rna Editing ◽  
Dna Sequence ◽  
Temporal Relationship ◽  
Editing Site ◽  
Differential Hybridization ◽  
Trans Splicing ◽  
Intron Removal ◽  
Cis And Trans ◽  
Relationship Of ◽  
Editing Process

Plant mitochondrial mRNAs have recently been shown to undergo editing, involving cytidine-to-uridine changes relative to the DNA sequence. We have examined the temporal relationship of editing and intron removal in coxII mRNAs in Petunia mitochondria. By using differential hybridization to probes specific for edited and unedited RNA and by sequencing of individual unspliced coxII pre-mRNA cDNAs, we found that RNA editing at any editing site can precede the splicing event. Similar results were obtained from examinations of pre-mRNA cDNAs of nad1, a gene composed of multiple exons that are both cis and trans spliced. Thus, intron removal is not required before editing can occur. The existence of editing intermediates indicates that the editing process is not strictly coincident with transcription.

Detection of DNA adducts at the DNA sequence level by ligation-mediated PCR

1993 ◽  
Vol 288 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Gerd P. Pfeifer ◽  
Régen Drouin ◽  
Gerald P. Holmquist
Keyword(s):  
Dna Sequence ◽  
Dna Adducts

scholarly journals Editing of pre-mRNAs can occur before cis- and trans-splicing in Petunia mitochondria.

1991 ◽  
Vol 11 (8) ◽  
pp. 4274-4277 ◽  
Author(s):  
C A Sutton ◽  
P L Conklin ◽  
K D Pruitt ◽  
M R Hanson
Keyword(s):  
Rna Editing ◽  
Dna Sequence ◽  
Temporal Relationship ◽  
Editing Site ◽  
Differential Hybridization ◽  
Trans Splicing ◽  
Intron Removal ◽  
Cis And Trans ◽  
Relationship Of ◽  
Editing Process

Plant mitochondrial mRNAs have recently been shown to undergo editing, involving cytidine-to-uridine changes relative to the DNA sequence. We have examined the temporal relationship of editing and intron removal in coxII mRNAs in Petunia mitochondria. By using differential hybridization to probes specific for edited and unedited RNA and by sequencing of individual unspliced coxII pre-mRNA cDNAs, we found that RNA editing at any editing site can precede the splicing event. Similar results were obtained from examinations of pre-mRNA cDNAs of nad1, a gene composed of multiple exons that are both cis and trans spliced. Thus, intron removal is not required before editing can occur. The existence of editing intermediates indicates that the editing process is not strictly coincident with transcription.

Komplexbildung und Ligandeneigenschaften der Chlormethylchlorphosphane R(Cl)P-CH2-Cl - Molekülstruktur von trans-Cl2Pd[tBu(Cl)P-CH2-Cl]2 / Coordination Chemistry and Ligand Properties of Chloromethylchlorophosphines R(Cl)P-CH2 -Cl - Molecular Structure of trans-Cl2Pd[tBu(Cl)P-CH2 -Cl]2

1997 ◽  
Vol 52 (9) ◽  
pp. 1103-1113 ◽  
Author(s):  
Peter Machnitzki ◽  
Othmar Stelzer ◽  
Claudia Landgrafe
Keyword(s):  
Heterogeneous Reaction ◽  
Trans Isomers ◽  
Triclinic Space Group ◽  
X Ray ◽  
H Nmr ◽  
Cis And Trans Isomers ◽  
Square Planar ◽  
High Yields ◽  
Cis And Trans ◽  
Trans Structure

Reaction of the chloromethylchlorophosphines R(Cl)P-CH2-Cl (R = Cl, tBu, C6H11, NEt2, NPh2) (L) with C7H8Mo(CO)4 or (CO)5W(Py), (CO)6W and (CO5W(THF) yields the complexes cis-Mo(CO)4L2(2a - 2e) or (CO)5 WL (3a - 3c), respectively, in high yields. The palladium(II) complexes PdCl2L2 (4a - 4c) are obtained on treatment of 1a, 1b and 1e with 1,5-cyclooctadiene palladium(II) chloride or anhydrous palladium(II) chloride in a homogeneous or heterogeneous reaction, respectively. 4a - 4c are formed as a mixture of cis- and trans-isomers. The 13C {1H} NMR and 1H NMR spectra of 2a - 4c have been analysed, the trans-structure of 4c could be assigned to the predominant isomer. The X-ray structural analysis of 4c (triclinic, space group Pl̅) reveals a trans-square planar coordination at palladium with an antiperiplanar arrangement of the bulky tBu substituents at the phosphorus atoms. Electronic (ƩXiMo) and steric ligand parameters (θ) are given for the chloromethylchlorophosphines R(Cl)P-CH2-Cl (R = Cl, tBu, C6H11, NEt2, NPh2).

scholarly journals Molecular analysis of mutations induced in the vermilion gene of Drosophila melanogaster by methyl methanesulfonate.

Genetics ◽  
1992 ◽  
Vol 131 (3) ◽  
pp. 673-682 ◽  
Author(s):  
M J Nivard ◽  
A Pastink ◽  
E W Vogel
Keyword(s):  
Drosophila Melanogaster ◽  
Germ Cell ◽  
Dna Sequence ◽  
Molecular Analysis ◽  
Dna Adducts ◽  
Strong Preference ◽  
Male Germ Cell ◽  
Methyl Methanesulfonate ◽  
Mutational Spectra ◽  
Related Process

Abstract The nature of DNA sequence changes induced by methyl methanesulfonate (MMS) at the vermilion locus of Drosophila melanogaster was determined after exposure of postmeiotic male germ cell stages. MMS is a carcinogen with strong preference for base nitrogen alkylation (s = 0.86). The spectrum of 40 intralocus mutations was dominated by AT----GC transitions (23%), AT----TA transversions (54%) and deletions (14%). The small deletions were preferentially found among mutants isolated in the F1 (8/18), whereas the AT----GC transitions exclusively occurred in the F2 (6/22). The MMS-induced transversions and deletions are presumably caused by N-methyl DNA adducts, which may release apurinic intermediates, known to be a time-related process. Furthermore, MMS produces multilocus deletions, i.e., at least 30% of the F1 mutants analyzed were of this type. A comparison of the mutational spectra of MMS with that produced by ethylnitrosourea (ENU), also in the vermilion locus of Drosophila, reveals major differences: predominantly transition mutations (61% GC----AT and 18% AT----GC) were found in both the F1 and F2 spectrum induced by ENU. It is concluded that the mutational spectrum of MMS is dominated by nitrogen DNA adducts, whereas with ENU DNA sequence changes mainly arose from modified oxygen in DNA.

DNA sequence mapping in interphase and metaphase chromosomes by fluorescence in situ hybridization

1992 ◽  
Vol 50 (1) ◽  
pp. 496-497
Author(s):  
Barbara Trask ◽  
Susan Allen ◽  
Anne Bergmann ◽  
Mari Christensen ◽  
Anne Fertitta ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Dual Band ◽  
Nick Translation ◽  
Metaphase Chromosomes ◽  
Band Pass ◽  
Texas Red ◽  
Fluorescent Spot

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.

Phylogenetic relationships ofSalvia(Lamiaceae) in China: Evidence from DNA sequence datasets

2012 ◽  
pp. n/a-n/a
Author(s):  
Qian-Quan Li ◽  
Min-Hui Li ◽  
Qing-Jun Yuan ◽  
Zhan-Hu Cui ◽  
Lu-Qi Huang ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Phylogenetic Relationships

A Simple Method for Preparing Fibrinogen

1966 ◽  
Vol 16 (01/02) ◽  
pp. 198-206 ◽  
Author(s):  
W Straughn ◽  
R. H Wagner
Keyword(s):  
Barium Sulfate ◽  
Caproic Acid ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Human Fibrinogen ◽  
Simple Method ◽  
High Yields ◽  
Marked Stability

SummaryA simple new procedure is reported for the isolation of canine, bovine, porcine, and human fibrinogen. Two molar β-alanine is used to precipitate fibrinogen from barium sulfate adsorbed plasma. The procedure is characterized by dependability and high yields. The material is 95% to 98% clottable protein but still contains impurities such as plasminogen and fibrin-stabilizing factor. Plasminogen may be removed by adsorption with charcoal. The fibrinogen preparations exhibit marked stability to freezing, lyophilization, and dialysis. Epsilon-amino-n-caproic acid and gamma-aminobutyric acid which were also studied have the property of precipitating proteins from plasma but lack the specificity for fibrinogen found with β-alanine.

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