A Simple Method for Preparing Fibrinogen
1966 ◽
Vol 16
(01/02)
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pp. 198-206
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Keyword(s):
SummaryA simple new procedure is reported for the isolation of canine, bovine, porcine, and human fibrinogen. Two molar β-alanine is used to precipitate fibrinogen from barium sulfate adsorbed plasma. The procedure is characterized by dependability and high yields. The material is 95% to 98% clottable protein but still contains impurities such as plasminogen and fibrin-stabilizing factor. Plasminogen may be removed by adsorption with charcoal. The fibrinogen preparations exhibit marked stability to freezing, lyophilization, and dialysis. Epsilon-amino-n-caproic acid and gamma-aminobutyric acid which were also studied have the property of precipitating proteins from plasma but lack the specificity for fibrinogen found with β-alanine.
1974 ◽
Vol 23
(17)
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pp. 2363-2368
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Keyword(s):
1964 ◽
Vol 11
(01)
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pp. 064-074
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Keyword(s):
2019 ◽
Vol 1
(1)
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pp. 6-12
Keyword(s):