Influence of soil pH on the development of symbiosis in field-grown acid-sensitive and acid-tolerant annual medics

1992 ◽  
Vol 32 (2) ◽  
pp. 167 ◽  
Author(s):  
RR Young ◽  
J Brockwell
Keyword(s):  
Soil Ph ◽  
Rhizobium Meliloti ◽  
New South ◽  
Annual Species ◽  
Resident Population ◽  
Growing Period ◽  
South Wales ◽  
Normal Expression ◽  
Acid Tolerant ◽  
Annual Medics

Relationships between soil pH, resident population of Rhizobium meliloti in the soil, rhizobial colonisation of the rhizosphere, and nodulation of annual species of Medicago were examined. Established swards of M. truncatula (acid-sensitive) and of M. murex and M. polymorpha (acid-tolerant) growing at 6 different locations in central and western New South Wales were sampled to measure soil pH, to enumerate the populations of R. meliloti resident in the soil and colonising plant rhizospheres, and to assess the extent of nodulation. Soil pH (0.01 mol CaCl2/L) varied from 4.70 to 7.25, soil populations from 3 to 100000 rhizobialg, and rhizosphere populations from 10 to >1 x 106 rhizobia/plant. Nodulation varied from poor to good, depending on growing period and location, but was consistently poorer on M. truncatula than on M. murex or M. polymorpha. Regression analysis showed a consistent and significant (P<0.01) relationship between rhizobial colonisation of the rhizosphere and extent of nodulation. There was also a significant (P<0.001) correlation over all 44 sampling sites between soil pH and the population of R. meliloti resident in the soil. The relationships between soil pH and rhizosphere colonisation, and between soil pH and nodulation, were significant for M. truncatula (P<0.01, P<0.02, respectively) but not for either M. murex or M. polymorpha. These data suggest that the range of soil pH at which M. truncatula was growing (4.80-7.25, but mainly 4.8-5.6) spanned the threshold at which unfavourable soil pH began to obstruct normal expression of its symbiosis, whereas, the critical soil pH levels for symbiotic development of M. murex and M. polymorpha were below this range.

Rhizobium as a factor in the re-establishment of legume-based pastures on clay soils of the wheat belt of north-western New South Wales

1998 ◽  
Vol 38 (6) ◽  
pp. 555 ◽  
Author(s):  
A. M. Bowman ◽  
D. M. Hebb ◽  
D. J. Munnich ◽  
J. Brockwell
Keyword(s):  
Nitrogen Fixation ◽  
Rhizobium Meliloti ◽  
New South ◽  
New South Wales ◽  
Clay Soils ◽  
Natural Fertility ◽  
Fixed Nitrogen ◽  
South Wales ◽  
Annual Medics ◽  
North Western

Summary. Populations of Rhizobium meliloti in self-mulching clay soils (Vertisols) at 48 sites on 27 properties in north-western New South Wales were classified according to number and ability to fix nitrogen with several species of Medicago. Rhizobia were counted using serial dilution, nodulation frequency, plant infection tests. Abilities of the soil populations to fix nitrogen were determined in the laboratory with whole-soil inoculation of Medicago seedlings in test tubes with shoots exposed to the atmosphere and roots within the tubes under bacteriological control, and in the field using a technique based on the natural abundance of 15N in the soil. The majority of soils contained >1000 cells of R. meliloti per gram. The major component of those populations fixed nitrogen with lucerne (Medicago sativa) and some components of some soils also fixed nitrogen with M. polymorpha, M. scutellata, M. littoralis, M. tornata, M. laciniata and Trigonella suavissima. However, a number of soils were located which contained few if any rhizobia effective in nitrogen fixation with M. polymorpha. Overall, the effectiveness of nitrogen fixation of the naturally occurring populations of R. meliloti in association with M. polymorpha, M. scutellata, M. littoralis and M. tornata was only 46% of the effectiveness of standard strains. At one particular site, where 10 lines of annual Medicago spp. were growing experimentally, fixed nitrogen as a proportion of shoot nitrogen averaged only 28%. At that site, there were no effective rhizobia for M. scutellata and it was wholly dependent on the soil as the source of its nitrogen. The results are discussed in relation to the need for a substantial input of legume nitrogen for restoring the natural fertility of self-mulching clay soils in degraded wheat lands of north-western New South Wales. It is suggested that lucerne, or perhaps other perennial Medicago spp., might fill this role better than annual medics such as M. polymorpha and M. scutellata that are more dependent than lucerne on specific strains of R. meliloti to meet their requirements for symbiotic nitrogen fixation.

Soil pH is a major determinant of the numbers of naturally occurring Rhizobium meliloti in non-cultivated soils in central New South Wales

1991 ◽  
Vol 31 (2) ◽  
pp. 211 ◽  
Author(s):  
J Brockwell ◽  
A Pilka ◽  
RA Holliday
Keyword(s):  
Soil Ph ◽  
Rhizobium Meliloti ◽  
New South ◽  
New South Wales ◽  
Acid Soils ◽  
Alkaline Soils ◽  
Naturally Occurring ◽  
South Wales ◽  
Dominant Soil ◽  
The Relationship

Measurements were made of soil pH, frequency of occurrence of annual species of Medicago (medics) and populations of Rhizobium meliloti at 84 sites on 7 dominant soil groups of the Macquarie region of central-western New South Wales. Over all sites, soil pH (0-10 cm; 1:5 soil: water) ranged from 5.26 to 8.07, medic frequency from 0 to 100% and most probable numbers of R. meliloti from undetectable to 675 000/g soil. There was a highly significant (P<0.001) relationship between soil pH and number of R. meliloti. Above pH 7.0, the mean soil population of R. meliloti was 89000/g; below pH 6.0, it was 37/g. Medics occurred most frequently on the more alkaline soils and with least frequency on the more acid soils, but the relationship between soil pH and medic frequency was weaker than between pH and R. meliloti number. Medics were more tolerant of low soil pH than their rhizobia were; at 2 sites, of pH 5.49 and 5.35, medics occurred at 100% frequency but R. meliloti was undetected. There was an indication of some acidification in these soils over a period of 35 years but this remains to be confirmed.

An exploratory survey of the ecology of Rhizobium meliloti in inland New South Wales and Queensland

1962 ◽  
Vol 13 (5) ◽  
pp. 864 ◽  
Author(s):  
FW Hely ◽  
J Brockwell
Keyword(s):  
Rhizobium Meliloti ◽  
New South ◽  
Plant Size ◽  
Annual Species ◽  
Test Species ◽  
High Frequencies ◽  
South Wales ◽  
Exploratory Survey ◽  
Dry Conditions ◽  
Medicago Sativa L

The ability of Rhizobium meliloti occurring naturally at 26 sites to nodulate three test species of Medicago was investigated. Bacteria able to nodulate Medicago sativa L. were found, in the absence of this host, at all sites sampled and frequently in appreciable numbers, even under dry conditions. Bacteria able to nodulate Medicago denticulata Willd. were found at 25 sites, but usually in lower numbers; they also occurred independently of this species. Organisms able to nodulate Medicago laciniata (L.) Mill. were detected at 18 sites but only in low numbers; they were found at eight sites in the absence of M. laciniata and were not detected at three sites where this medic was present. High numbers of bacteria able to nodulate M. sativa and M. denticulata were related to high frequencies of Medicago minima (L.) Bart. in the field. Associations were found between high numbers of Rh. Meliloti in the topsoil and the texture of the soil, the plant size of the annual species of Medicago in the sample area, and the condition of the native grasses.

Differential rhizobial colonisation of the roots of sown and volunteer annual species of Medicago in an acid soil

1994 ◽  
Vol 34 (6) ◽  
pp. 745 ◽  
Author(s):  
RM Barclay ◽  
DM Hebb ◽  
J Brockwell
Keyword(s):  
Rhizobium Meliloti ◽  
Acid Soil ◽  
Arid Environment ◽  
Plant Infection ◽  
South Wales ◽  
Large Populations ◽  
Specific Strain ◽  
Acid Tolerant ◽  
Red Earth ◽  
Annual Medics

Two annual medics, Medicago murex and M. truncatula, were inoculated with specific strains of Rhizobium meliloti, WSM540 and CC169, respectively, and sown into a mildly acid red earth in a semi-arid environment at Cobar, New South Wales. A third medic, M. laciniata, volunteered abundantly at the experimental site and large populations of its specific strain(s) of R. meliloti were naturalised in the soil. Serial dilution, plant infection tests, using 3 test plants in parallel, were employed to count the populations of each of the 3 types of R. meliloti that colonised the roots (rhizospheres) of the 3 medics. The size of the rhizosphere populations was regarded as an index of nodulation potential. Large populations of the naturalised rhizobia (about 45000lplant) developed in the rhizospheres of M. laciniata; these rhizobia also colonised the root surfaces of the sown medics but in much lower numbers (< 80/plant). Strain WSM540 colonised the rhizospheres of M. murex in sufficient numbers (1121 rhizobia/plant) to indicate that an effective nitrogen-fixing symbiosis would have established had seasonal conditions permitted. By contrast, the colonisation of M. truncatula rhizospheres by CC169 was significantly lower (64 rhizobia/plant; P<0.05) and the likelihood of nodulation was uncertain. Both WSM540 and M. murex are known to be acid tolerant whereas both CC169 and M. truncatula are acid sensitive. There was very little colonisation of the rhizospheres of the sown medics by non-specific inoculant strains. Nor was there evidence that the large naturalised population of rhizobia for M. laciniata competed with the specific inoculant strains for colonisation of the roots of the sown medics or interfered with their potential nodulation.

Factors Associated With Density of Mitchell Grass Pastures in North-Western New South Wales.

1997 ◽  
Vol 19 (1) ◽  
pp. 40 ◽  
Author(s):  
AM Bowman ◽  
DJ Munnich ◽  
KY Chan ◽  
J Brockwell
Keyword(s):  
Plant Density ◽  
Rhizobium Meliloti ◽  
New South ◽  
New South Wales ◽  
Good Condition ◽  
Grazing Pressure ◽  
Annual Species ◽  
Basal Diameter ◽  
Poor Condition ◽  
South Wales

Thirty-eight paddocks on 23 properties in the Walgett Shire of north-westem New South Wales were surveyed in April and May 1993 to identify factors that affected Mitchell grass (Astrebla lappacea) plant density. Low densities of Mitchell grass were associated with the occurrence of at least one flood in the last 20 years. Soils from paddocks described by landholders as in poor condition, and where Mitchell grass density was low, were more alkaline and had significantly higher electrical conductivity than soils from paddocks described as in good condition, and where Mitchell grass density was high. Paddocks in poor condition had higher organic carbon and mineralisable nitrogen levels and higher soil populations of Rhizobium meliloti. These latter characteristics were attributed to these pastures having a high component of annual species, including Medicago species, which returned substantial amounts of carbon and nitrogen to the soil. Where Mitchell grass density was moderate, other palatable species were common and biodiversity was high. However, in paddocks with the lowest densities of Mitchell grass, both annual species and unpalatable perennials were common. Low basal diameter of Mitchell grass plants and low Mitchell grass density, were associated with higher grazing pressure. The data suggested that the Mitchell grass ecosystem in north-westem New South Wales is prone to deterioration on the more alkaline and saline soils and where inundation by flooding or waterlogging is common.

Evaluation of pasture legumes sown into a prepared seedbed at Tamworth, New South Wales. 2. Seed production and seed reserves

1993 ◽  
Vol 33 (3) ◽  
pp. 299 ◽  
Author(s):  
GM Lodge
Keyword(s):  
Seed Production ◽  
New South ◽  
New South Wales ◽  
Pasture Legumes ◽  
South Wales ◽  
Medicago Scutellata ◽  
Seed Reserves ◽  
Annual Medics ◽  
Perennial Legumes ◽  
Seed Yields

Plots sown in 1983 were used to examine the seed production and reserves (residual hardseeds) of 15 annual legumes over 5 years at Tamworth in northern New South Wales. Seed production characteristics were measured in 1983 for these annuals, and for 6 perennial legumes. After the annuals had set seed in 1983, an area of the plots was sprayed to prevent flowering in subsequent years, and the rates of decline in seed reserves were compared with those from areas that seeded annually. Seed yields of the perennials were often significantly lower than those of the annuals except Trifolium glomeratum. Seed yields of T. subterraneum var. subterraneum cvv. Seaton Park and Woogenellup were significantly higher than those of cv. Nungarin and T. subterrarzeum var. brachycalycinum cv. Clare. Seed numbers were lowest for Medicago scutellata cv. Sava among the annual medics, and for Nungarin among subterranean clovers. There was little relationship between the mean number of seeds produced from 1983-86 and maturity grading, and between seed numbers and relative dry matter yield. Seed reserves decreased over 5 years by more than 90% in the sprayed treatments of all species. This decrease was not continuous, with the largest declines occurring from December 1983 to August 1984. In the sprayed treatments of Seaton Park, Woogenellup, and Clare, and in both treatments of Astragalus hamosus cv. Ioman, Vicia dasycarpa var. villosa cv. Namoi, and T. hirtum cv. Hykon, none of the original 1983 seed was recovered in 1987. For the annual medics and Nungarin, the number of residual hardseeds in the sprayed treatments in 1987 was about 3-5% of the seed produced in 1983. From 1983 to 1987, seed numbers in the unsprayed treatments declined by 7040% for the annual medics and by 85-95% for Seaton Park, Woogenellup, and Clare. Long-term persistence of annual medics and Nungarin depended on seed production in most years and the maintenance of a high number of residual hardseeds in the soil. In contrast, the mid- and late-maturing subterranean clovers Woogenellup and Clare had low seed reserves and were dependent on seed production in most years for their continued regeneration.

Effect of soil pH on the distribution ofDanthoniaspecies on the tablelands of central and southern New South Wales

1996 ◽  
Vol 39 (4) ◽  
pp. 619-621 ◽  
Author(s):  
P. M. Dowling ◽  
D. L. Garden ◽  
D. A. Eddy ◽  
D. I. Pickering
Keyword(s):  
Soil Ph ◽  
New South ◽  
New South Wales ◽  
South Wales

Corrigendum - Frequency of annual species of Medicago on the major soil groups of the Mcquarie region of New South Wales

1960 ◽  
Vol 11 (5) ◽  
pp. 705
Author(s):  
WD Andrew ◽  
FW Hely
Keyword(s):  
New South ◽  
New South Wales ◽  
Salt Content ◽  
Acid Group ◽  
Soluble Salt ◽  
Annual Species ◽  
South Wales ◽  
Soil Groups ◽  
Soil Habitat ◽  
Poor Drainage

The frequency of eight annual species of Medicago was determined on 10 soil groups in a region climatically favourable for medics. They occurred on nine of these, the overall frequency being in the order M. minima (L.) Bart. > M. denticulata Willd. > M. laciniata (L.) Mill., M. praecox DC., M. tribuloides Desr., and M. orbicularis (L.) Bart. > M. arabica (L.) Huds. and M. confinis Koch. M. minima was more tolerant of variation of soil habitat than any other medic in the region. M. denticulata was most commonly found on the soils of high soluble salt content and showed the greatest tolerance of poor drainage conditions. M. laciniata occurred in greatest abundance on soils of the well-drained brown acid group. M. praecox was most commonly found on soils with a compact gravelly surface. M. tribuloides was recorded on five soil groups but was common only on the black earths. M. orbicularis was confined to the black earth and red loam soils. M. arabica was rarely found, being restricted to lower, fertile, well-drained parts of the landscape, and M. confinis occurred only occasionally and then in association with M. denticulata.

Long-term benefits of limestone applications to soil properties and to cereal crop yields in southern and central New South Wales

2003 ◽  
Vol 43 (1) ◽  
pp. 71 ◽  
Author(s):  
M. K. Conyers ◽  
C. L. Mullen ◽  
B. J. Scott ◽  
G. J. Poile ◽  
B. D. Braysher
Keyword(s):  
Grain Yield ◽  
New South ◽  
New South Wales ◽  
Commercial Application ◽  
South Wales ◽  
Cereal Grain ◽  
Subsurface Soil ◽  
Acid Tolerant ◽  
The Cost

The cost of buying, carting and spreading limestone, relative to the value of broadacre crops, makes investment in liming a questionable proposition for many farmers. The longer the beneficial effects of limestone persist, however, the more the investment in liming becomes economically favourable. We re-established previous lime trials with the aim of measuring the long-term effects of limestone on surface acidity (pH run-down), subsurface acidity (lime movement) and grain yield. The study made use of experiments where there was adequate early data on soil chemical properties and cereal yields. We report data from 6 trials located at 4 sites between Dubbo and Albury in New South Wales. The rate of surface soil (0–10 cm) pH decline after liming was proportional to the pH attained 1 year after liming. That is, the higher the pH achieved, the more rapid the rate of subsequent pH decline. Since yields (product removal) and nitrification (also acid producing) may both vary with pH, the post-liming pH acts as a surrogate for the productivity and acid-generating rate of the soil–plant system. The apparent lime loss rate of the surface soils ranged from the equivalent of nearly 500 kg limestone/ha.year at pH approaching 7, to almost zero at pH approaching 4. At commercial application rates of 2–2.5 t/ha, the movement of alkali below the layer of application was restricted. However, significant calcium (Ca) movement sometimes occurred to below 20 cm depth. At rates of limestone application exceeding the typical commercial rate of 2.5 t/ha, or at surface pH greater than about 5.5, alkali and Ca movement into acidic subsurface soil was clearly observed. It is therefore technically feasible to ameliorate subsurface soil acidity by applying heavy rates of limestone to the soil surface. However, the cost and risks of this option should be weighed against the use of acid-tolerant cultivars in combination with more moderate limestone rates worked into the surface soil.There was a positive residual benefit of limestone on cereal grain yield (either barley, wheat, triticale, or oats) at all sites in both the 1992 and 1993 seasons. While acid-tolerant cultivars were less lime responsive than acid-sensitive ones, the best yields were generally obtained using a combination of liming and acid-tolerant cultivars.The long-term residual benefits of limestone were shown to extend for beyond 8–12 years and indicate that liming should be profitable in the long term.

The effect of biosolids on cereals in central New South Wales, Australia. 1. Crop growth and yield

2005 ◽  
Vol 45 (4) ◽  
pp. 435 ◽  
Author(s):  
J. L. Cooper
Keyword(s):  
Sewage Sludge ◽  
Soil Ph ◽  
New South ◽  
New South Wales ◽  
Acid Soils ◽  
Crop Growth ◽  
Growth And Yield ◽  
South Wales ◽  
Sludge Cake ◽  
Dewatered Sewage Sludge

Two forms of biosolids, with and without lime, were applied to acid soils at 2 sites in central New South Wales. Wheat and triticale were then grown on these sites to determine the effect of biosolids on crop growth and yield. The forms of biosolids used were dewatered sewage sludge cake, and N-Viro Soil which is a lime amended sewage sludge. Dewatered sewage sludge cake was applied at rates of 0, 6, 12 and 24 dry Mg/ha, and N-Viro soil at 0, 1.5, 3.0 and 4.5 dry Mg/ha. Biosolids produced grain yield increases of over 50% at both sites, with the largest yield increases at the highest rate of dewatered sewage sludge. Continued cropping at 1 of the sites showed that significant yield increases were still obtained 3 years after the initial application. The addition of lime and N-Viro Soil raised soil pH, and produced small but long lasting yield increases. However, the main benefit of biosolids seems to have come from the nutrients they supplied rather than changes in soil pH.

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