Effect of different pasture legumes on growth profile and forage production of the selected native pasture grasses mix at different growth stages

The present experiment aimed to investigate the effect of introducing different pasture legumes on the growth profile and forage production of the selected native pasture grass species at different stages of growth. In a completely randomized design with 5 treatments and 5 replications, the mixture of Sorghum plumosum (SP) and Bothriochloa pertusa (BP) was introduced respectively with one of the forage legumes ie. Alysicarpus vaginalis (AV), Pueraria phasoloides (PP), Desmodium incanum (DI), and Clitoria ternatea (CT). Growth profile and forage production were measured at 40, 60, and 80 days after planting. Results showed that CT and PP significantly improved the growth and DM production of SP and suppressed (P<0.05) the growth of BP during the early vegetative stage but did not during the late vegetative stage. Introduction of legumes reduced (P<0.05) DM production of SP and the total forage production but improved (P<0.001) the DM production of B. pertusa as well as a leaf:stem ratio of both types of grass at the generative stage. PP had the highest (P<0.05) contribution of legumes to the total DM forage production during early and vegetative stages, meanwhile AV and DI during the generative stage. In conclusion, the introduction of forage legumes did not improve the DM production of both grass species but modify their growth profile toward a better quality as shown by increased leaf:stem ratio. P. phasoloides provide the highest foliage during the vegetative stage and A. vaginalis and D. incanum during the generative stage.

Overexpression of SgGH3.1 from Fine-Stem Stylo (Stylosanthes guianensis var. intermedia) Enhances Chilling and Cold Tolerance in Arabidopsis thaliana

Stylosanthes (stylo) species are commercially significant tropical and subtropical forage and pasture legumes that are vulnerable to chilling and frost. However, little is known about the molecular mechanisms behind stylos’ responses to low temperature stress. Gretchen-Hagen 3 (GH3) proteins have been extensively investigated in many plant species for their roles in auxin homeostasis and abiotic stress responses, but none have been reported in stylos. SgGH3.1, a cold-responsive gene identified in a whole transcriptome profiling study of fine-stem stylo (S. guianensis var. intermedia) was further investigated for its involvement in cold stress tolerance. SgGH3.1 shared a high percentage of identity with 14 leguminous GH3 proteins, ranging from 79% to 93%. Phylogenetic analysis classified SgGH3.1 into Group Ⅱ of GH3 family, which have been proven to involve with auxins conjugation. Expression profiling revealed that SgGH3.1 responded rapidly to cold stress in stylo leaves. Overexpression of SgGH3.1 in Arabidopsis thaliana altered sensitivity to exogenous IAA, up-regulated transcription of AtCBF1-3 genes, activated physiological responses against cold stress, and enhanced chilling and cold tolerances. This is the first report of a GH3 gene in stylos, which not only validated its function in IAA homeostasis and cold responses, but also gave insight into breeding of cold-tolerant stylos.

Characterization of Phytoestrogens in Medicago sativa L. and Grazing Beef Cattle

Phytoestrogens are plant-produced bioactive secondary metabolites known to play an integral role in plant defense that frequently accumulate in times of stress and/or microbial infection. Phytoestrogens typically belong to two distinct chemical classes; flavonoids (isoflavones) and non-flavonoids (lignans and coumestans). Upon consumption by livestock, high concentrations of phytoestrogens can cause long-term disruption in reproduction due to structural similarities with mammalian estrogens and their tendency to bind estrogen receptors. Wide variation in phytoestrogen concentration has been reported in pasture legumes and corresponding silage or hay. Lucerne is a common perennial pasture legume in temperate climates, but information on phytoestrogen production or accumulation in grazing livestock is currently limited. Therefore, metabolic profiling using UHPLC-MS-QToF was performed to identify and quantitate key phytoestrogens in both fresh and dried lucerne fodder from replicated field or controlled glasshouse environments. Phytoestrogens were also profiled in the blood plasma of Angus cattle grazing field-grown lucerne. Results revealed that phytoestrogens varied quantitatively and qualitatively among selected lucerne cultivars grown under glasshouse conditions. Fresh lucerne samples contained higher concentrations of coumestans and other phytoestrogenic isoflavones than did dried samples for all cultivars profiled, with several exceeding desirable threshold levels for grazing cattle. Coumestans and isoflavones profiled in plasma of Angus heifers grazing lucerne increased significantly over a 21-day sampling period following experimental initiation. Currently, threshold concentrations for phytoestrogens in plasma are unreported. However, total phytoestrogen concentration exceeded 300 mg·kg−1 in fresh and 180 mg·kg−1 in dried samples of selected cultivars, suggesting that certain genotypes may upregulate phytoestrogen production, while others may prove suitable sources of fodder for grazing livestock.

Landscape position, sampling time and tillage, but not legume species, affect labile carbon and nitrogen fractions in a four-year-old rejuvenated grazed pasture

Termination by tillage is one strategy used for regenerating pasture stands. Yet, research gaps exist on how tillage affects carbon (C) and nitrogen (N) forms and amounts in western Canadian soils. We measured total soil organic C (SOC), dissolved organic C (DOC), total dissolved N (TDN), light fraction organic C (LFOC) and N (LFON), microbial biomass C (MBC) and N (MBN), and inorganic N as indicators of soil organic matter (SOM) dynamics. After tillage termination in fall 2018, we sampled soils (0‒10cm; 0‒15cm) under three legume species (alfalfa, cicer milkvetch and sainfoin) three times (spring, summer and fall of 2019) across three landscape positions. Legume species did not affect the measured parameters. Over time, tillage affected DOC, TDN, and inorganic N. Averaged across three pasture legumes and three landscape positions, tillage increased DOC 29% by summer. Fall-applied tillage led to 59% and 33% higher TDN in the succeeding summer and fall. Inorganic N increased by 14% and 40% across landscape positions and sampling after tillage. Averaged across landscape positions, MBC decreased by 31% from spring to summer and increased by 51% from summer to fall. However, MBN increased by 53% and decreased by 5% within the same period. The seasonal fluctuations in MBC/MBN reflected variations in moisture, temperature, and substrate quality. Total SOC, LFOC, and LFON increased on the upper slopes and fall sampling time. Although single intensive tillage did not affect total SOC, several tillage operations could accelerate SOM loss and reduced total C storage over time.

Delivering improved phosphorus acquisition by root systems in pasture and arable crops

Improving low efficiency of phosphorus (P) use in agriculture is an imperative because P is one of the key nutrients underpinning sustainable intensification of food production and the rock-phosphate reserves, from which P fertilisers are made, are finite. This paper describes key soil, root and microbial processes that influence P acquisition with a focus on factors that can be managed to ensure optimal use of fertiliser, and development of root systems for improved P acquisition. A case study describes grasslands in southern Australia where the P-balance efficiency of production is very low, mainly because soils are P deficient and moderately to highly P-sorbing. Use of soluble P fertiliser, P-banding and soil testing to guide soil P management ensures effective use of P fertiliser. Progress towards improved P efficiency using pasture legumes with high P-acquisition efficiency is outlined. Development of a ‘whole-of-system’ understanding for effective P acquisition by roots is highlighted.

Investigation of two native Australian perennial forage legumes for their potential use in agriculture: Indigofera australis subsp. hesperia and Glycyrrhiza acanthocarpa

In order to investigate the potential for domestication of native pasture legumes, a seed collecting mission was undertaken between Kalbarri and Esperance in the south of Western Australia followed by establishment of a field nursery at Northam, Western Australia. Indigofera australis subsp. hesperia Peter G.Wilson &amp; Rowe was collected from eight sites, Indigofera brevidens Benth. from one site, and Glycyrrhiza acanthocarpa (Lindl.) J.M.Black from six sites. The field nursery was an irrigated, replicated trial designed to produce seed for future field testing and to provide preliminary information on plant agronomic characteristics. Over 12 months, I. australis produced herbage dry matter (DM) of 2.1–4.4 t/ha compared with 3.4 t/ha for a composite line of tedera (Bituminaria bituminosa C.H. Stirton vars. albomarginata and crassiuscula) and 1.0 t/ha DM for G. acanthocarpa. Most lines had digestibility and crude protein (15–25%) values that would support moderate growth of sheep or cattle. The best line of G. acanthocarpa produced &gt;400 kg/ha of seeds, whereas I. australis had poor production most likely due to poor adaptation to the nursery site. The severity of cutting of I. australis plants had no significant effect on visual assessments of herbage growth. Most plants of I. australis died within 3 years, whereas most plants of G. acanthocarpa, I. brevidens and tedera survived for 3 years. Both I. australis and G. acanthocarpa have potential for use in broadacre agriculture as grazing plants, most likely in niches specifically suited to each, and demonstrate that native plants can contribute to pasture production currently dominated by exotic species.

Intrinsic root morphology determines the phosphorus acquisition efficiency of five annual pasture legumes irrespective of mycorrhizal colonisation

Mycorrhizal fungi are ubiquitous in agroecosystems and form symbiotic associations that contribute to the phosphorus (P) acquisition of many plants. The impact of mycorrhizas is most pronounced in P-deficient soil and commonly involves modifications to the root morphology of colonised plants. However, the consequences of mycorrhizal colonisation on root acclimation responses to P stress are not well described. Five annual pasture legumes, with differing root morphologies, were grown to determine the effect of mycorrhizal colonisation on shoot yield, root morphology and P uptake. Micro-swards of each legume were established in pots filled with a topsoil layer that had been amended with five rates of P fertiliser. The topsoil overlaid a low-P subsoil that mimicked the stratification of P that occurs under pasture. Mycorrhizal colonisation improved P acquisition and shoot yield in the low-P soil treatments, but did not reduce the critical external P requirement of the legumes for near-maximum yield. The yield responses of the mycorrhizal plants were associated with reduced dry matter allocation to topsoil roots, which meant that the P acquisition benefit associated with mycorrhizal colonisation was not additive in the P-deficient soil. The contribution of the mycorrhizal association to P acquisition was consistent among the legumes when they were compared at an equivalent level of plant P stress, and was most pronounced below a P stress index of ~0.5. The intrinsic root morphology of the legumes determined their differences in P-acquisition efficiency irrespective of mycorrhizal colonisation.

Productivity of perennial herbs on peat-mineral soils in the conditions of Polesie

The results of studies on the productivity of perennial grasses on peat-mineral soil of Polesye are presented. Sowing alfalfa turned out to be the most productive crop among perennial leguminous herbs. Pasture legumes and grasses were slightly different in productivity. The introduction of an additional bean component in pasture mixtures did not contribute to a significant increase in the productivity of perennial herbs.