scholarly journals Systems biology: a new paradigm for industrial yeast strain development

2011 ◽  
Vol 32 (4) ◽  
pp. 151
Author(s):  
Cristian Varela ◽  
Simon A Schmidt ◽  
Anthony R Borneman ◽  
Jens O Kromer ◽  
Alamgir Khan ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Systems Biology ◽  
Yeast Strain ◽  
Biological Processes ◽  
Industrial Yeast ◽  
Strain Development ◽  
New Paradigm ◽  
Complex Interactions ◽  
Industrial Strains ◽  
Industrial Yeast Strain

One of the key challenges for industrial yeast strain development is to obtain a thorough understanding of the biology of yeast and to apply this knowledge to develop novel strains with improved features. The detailed study of individual biological components and the use of metabolic engineering have benefited the development of industrial strains enormously; however, such approaches have failed to describe yeast behaviour in the detail required to reveal the complex interactions operating within such biological systems. How can we accurately describe the biological processes and the interactions that occur during fermentation or cell growth?

Genetic characterization and construction of an auxotrophic strain of Saccharomyces cerevisiae JP1, a Brazilian industrial yeast strain for bioethanol production

2012 ◽  
Vol 39 (11) ◽  
pp. 1673-1683 ◽  
Author(s):  
Viviane Castelo Branco Reis ◽  
André Moraes Nicola ◽  
Osmar de Souza Oliveira Neto ◽  
Vinícius Daniel Ferreira Batista ◽  
Lidia Maria Pepe de Moraes ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Strain ◽  
Genetic Characterization ◽  
Bioethanol Production ◽  
Industrial Yeast ◽  
Auxotrophic Strain ◽  
Industrial Yeast Strain

scholarly journals Engineered Trx2p industrial yeast strain protects glycolysis and fermentation proteins from oxidative carbonylation during biomass propagation

2012 ◽  
Vol 11 (1) ◽  
pp. 4 ◽  
Author(s):  
Rocío Gómez-Pastor ◽  
Roberto Pérez-Torrado ◽  
Elisa Cabiscol ◽  
Joaquim Ros ◽  
Emilia Matallana
Keyword(s):  
Yeast Strain ◽  
Oxidative Carbonylation ◽  
Industrial Yeast ◽  
Industrial Yeast Strain

Industrial yeast strain engineered to ferment ethanol from lignocellulosic biomass

2011 ◽  
Vol 102 (17) ◽  
pp. 8310-8313 ◽  
Author(s):  
Nikolai Khramtsov ◽  
Luise McDade ◽  
Alexander Amerik ◽  
Esther Yu ◽  
Kunjan Divatia ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Yeast Strain ◽  
Industrial Yeast ◽  
Industrial Yeast Strain

scholarly journals How to characterize a strain? The neglected influence of clonal heterogeneity on the phenotypes of industrial Saccharomyces

2020 ◽  
Author(s):  
Hanna Viktória Rácz ◽  
Fezan Mukhtar ◽  
Alexandra Imre ◽  
Zoltán Rádai ◽  
Andreas Károly Gombert ◽  
...  
Keyword(s):  
Single Cell ◽  
Yeast Strain ◽  
Genome Structure ◽  
Clonal Lineage ◽  
Industrial Yeast ◽  
Clonal Interference ◽  
Clonal Heterogeneity ◽  
Colony Area ◽  
Industrial Yeast Strain ◽  
Industrial Yeasts

AbstractPopulations of microbes are constantly evolving heterogeneity that selection acts upon, yet heterogeneity is non-trivial to assess methodologically. The practice of isolating single cell colonies for establishing, transferring, and using a strain results in single-cell bottlenecks with a generally neglected effect on the characteristics of the strain. We used six industrial yeasts to assess the level of heterogeneity in clonal populations, especially in terms of stress tolerance. First, we uncovered the existence of genome structure variants in available sequenced genomes of clonal lineages of thes strains. Subsequent phenotyping of strains and their newly isolated subclones showed that single-cell bottlenecks during isolation can considerably influence the observable phenotype. Next, we decoupled fitness distributions on the level of individual cells from clonal interference by plating single cell colonies. We used the obtained data on colony area for statistical modeling of the heterogeneity in phenotypes. One strain was further used to show how individual subclonal lineages are remarkably different not just in phenotype, but also in the level of heterogeneity. Thereby we call attention to the fact that choosing an initial clonal lineage from an industrial yeast strain may vastly influence downstream performances and observations on geno- and phenotype, and also on heterogeneity.

scholarly journals INTRODUCTION OF FLOCCULATION INTO AN INDUSTRIAL YEAST STRAIN BY TRANSFER OF A SINGLE CHROMOSOME

1992 ◽  
Vol 98 (4) ◽  
pp. 315-319 ◽  
Author(s):  
Françoise Vezinhet ◽  
Pierre Barre ◽  
Monique Laurent ◽  
Michel Valade
Keyword(s):  
Yeast Strain ◽  
Industrial Yeast ◽  
Single Chromosome ◽  
Industrial Yeast Strain
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