Aromatase gene expression in immature rat Sertoli cells: age-related changes in the FSH signalling pathway

2010 ◽  
Vol 22 (3) ◽  
pp. 508 ◽  
Author(s):  
Hélène Bouraïma-Lelong ◽  
Marion Vanneste ◽  
Christelle Delalande ◽  
Leïla Zanatta ◽  
Slawomir Wolczynski ◽  
...  
Keyword(s):  
Gene Expression ◽  
Sertoli Cells ◽  
Post Partum ◽  
Signalling Pathway ◽  
Aromatase Gene ◽  
Age Related ◽  
Negative Effect ◽  
Old Rats ◽  
Aromatase Gene Expression

Aromatase, the enzyme responsible for the transformation of androgens into oestrogens, is encoded by the cyp19 gene expressed in the testis. The aim of the present study was to analyse the evolution of aromatase gene expression under FSH control in rat Sertoli cells between 10 and 30 days post partum, corresponding to the end of the proliferative period of Sertoli cells, establishment of the blood–testis barrier and acquisition of the mature phenotype. The maximum stimulatory effect of FSH on aromatase gene expression was obtained in 20-day-old rat Sertoli cells, compared with cells from 10- and 30-day-old rats, in parallel with the differentiation of Sertoli cells. Using two effectors of the protein kinase A pathway (i.e. forskolin and dibutyryl-cAMP) revealed differential effects between cells from rats aged 20 and 30 days, implying the involvement of another signalling pathway. Experiments using the specific phosphatidylinositol 3-kinase (PI3-K) inhibitor LY294002 revealed that PI3-K is strongly involved in FSH-induced aromatase expression in Sertoli cells from both 20- and 30-day-old rats. In vivo, this decrease could be explained by a negative effect exerted by germ cells because, in coculture, aromatase gene expression in 20-day-old Sertoli cells is greatly diminished.

Quantification of P450 aromatase gene expression in cultured and in vivo grown ovarian follicles in sheep

2014 ◽  
Vol 117 (1) ◽  
pp. 66-72 ◽  
Author(s):  
B.N.V. Lakshminarayana ◽  
V. Praveen Chakravarthi ◽  
K.V. Brahmaiah ◽  
V.H. Rao
Keyword(s):  
Gene Expression ◽  
Ovarian Follicles ◽  
Aromatase Gene ◽  
P450 Aromatase ◽  
Aromatase Gene Expression

Regulation of aromatase expression by 1α,25(OH)2 vitamin D3 in rat testicular cells

2011 ◽  
Vol 23 (5) ◽  
pp. 725 ◽  
Author(s):  
Leila Zanatta ◽  
Hélène Bouraïma-Lelong ◽  
Christelle Delalande ◽  
Fátima R. M. B. Silva ◽  
Serge Carreau
Keyword(s):  
Gene Expression ◽  
Vitamin D ◽  
Vitamin D Receptor ◽  
Vitamin D3 ◽  
Sertoli Cells ◽  
Endocrine System ◽  
Aromatase Gene ◽  
Testicular Cells ◽  
Pka Pathway ◽  
Aromatase Gene Expression

It is well known that the vitamin D endocrine system is involved in physiological and biochemical events in numerous tissues, especially gut, bone and kidney but also testis. Therefore, in this study the effect and mechanisms of action of 1α,25(OH)2 vitamin D3 (1,25D) on aromatase gene expression in immature rat Sertoli cells were evaluated. Vitamin D receptor transcripts were present in immature Sertoli cells as well as in adult testicular germ cells and somatic cells. The treatment of immature Sertoli cells with 100 nM 1,25D increased the amount of aromatase transcript, mainly in 30-day-old rats. The protein kinase A (PKA) blocker, H89, partially inhibited the 1,25D effect. The stimulation of aromatase gene expression in 30-day-old Sertoli cells by the agonist 1α,25(OH)2 lumisterol3, and the suppression of the 1,25D effect by the antagonists 1β,25(OH)2 vitamin D3 and (23S)-25-dehydro-1α (OH)-vitamin D3-26,23-lactone suggested, besides a genomic effect of 1,25D, the existence of non-genomic activation of the membrane-bound vitamin D receptor involving the PKA pathway.

Longitudinal force and stress of rat's esophagus: age-related changes.

1977 ◽  
Vol 232 (6) ◽  
pp. E580
Author(s):  
M P Zabinski ◽  
P Biancani
Keyword(s):  
Age Groups ◽  
Longitudinal Direction ◽  
Longitudinal Force ◽  
Active Stress ◽  
Length Relationship ◽  
Age Related ◽  
Old Rats ◽  
The Difference

Longitudinal force-length relationship of the rat esophagus was studied in vitro in three age groups: 1 mo, 3 mo, and 12 mo. The length of maximum force development (MFD) occurs at 1.4-1.5 times the in vivo length for all age groups. The active force developed at MFD increases markedly with age. The difference in the active forces in the 3-mo and 12-mo age groups is due to differences in cross section because the active stress of the esophagus in the longitudinal direction is approximately equal for the two age groups. The active stress in the 1-mo-old rats is lower than in the 3-mo-old rats, suggesting an increased contractility of the esophagus with age in this period of development.

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