Regulation of aromatase expression by 1α,25(OH)2 vitamin D3 in rat testicular cells

2011 ◽  
Vol 23 (5) ◽  
pp. 725 ◽  
Author(s):  
Leila Zanatta ◽  
Hélène Bouraïma-Lelong ◽  
Christelle Delalande ◽  
Fátima R. M. B. Silva ◽  
Serge Carreau
Keyword(s):  
Gene Expression ◽  
Vitamin D ◽  
Vitamin D Receptor ◽  
Vitamin D3 ◽  
Sertoli Cells ◽  
Endocrine System ◽  
Aromatase Gene ◽  
Testicular Cells ◽  
Pka Pathway ◽  
Aromatase Gene Expression

It is well known that the vitamin D endocrine system is involved in physiological and biochemical events in numerous tissues, especially gut, bone and kidney but also testis. Therefore, in this study the effect and mechanisms of action of 1α,25(OH)2 vitamin D3 (1,25D) on aromatase gene expression in immature rat Sertoli cells were evaluated. Vitamin D receptor transcripts were present in immature Sertoli cells as well as in adult testicular germ cells and somatic cells. The treatment of immature Sertoli cells with 100 nM 1,25D increased the amount of aromatase transcript, mainly in 30-day-old rats. The protein kinase A (PKA) blocker, H89, partially inhibited the 1,25D effect. The stimulation of aromatase gene expression in 30-day-old Sertoli cells by the agonist 1α,25(OH)2 lumisterol3, and the suppression of the 1,25D effect by the antagonists 1β,25(OH)2 vitamin D3 and (23S)-25-dehydro-1α (OH)-vitamin D3-26,23-lactone suggested, besides a genomic effect of 1,25D, the existence of non-genomic activation of the membrane-bound vitamin D receptor involving the PKA pathway.

Aromatase gene expression in immature rat Sertoli cells: age-related changes in the FSH signalling pathway

2010 ◽  
Vol 22 (3) ◽  
pp. 508 ◽  
Author(s):  
Hélène Bouraïma-Lelong ◽  
Marion Vanneste ◽  
Christelle Delalande ◽  
Leïla Zanatta ◽  
Slawomir Wolczynski ◽  
...  
Keyword(s):  
Gene Expression ◽  
Sertoli Cells ◽  
Post Partum ◽  
Signalling Pathway ◽  
Aromatase Gene ◽  
Age Related ◽  
Negative Effect ◽  
Old Rats ◽  
Aromatase Gene Expression

Aromatase, the enzyme responsible for the transformation of androgens into oestrogens, is encoded by the cyp19 gene expressed in the testis. The aim of the present study was to analyse the evolution of aromatase gene expression under FSH control in rat Sertoli cells between 10 and 30 days post partum, corresponding to the end of the proliferative period of Sertoli cells, establishment of the blood–testis barrier and acquisition of the mature phenotype. The maximum stimulatory effect of FSH on aromatase gene expression was obtained in 20-day-old rat Sertoli cells, compared with cells from 10- and 30-day-old rats, in parallel with the differentiation of Sertoli cells. Using two effectors of the protein kinase A pathway (i.e. forskolin and dibutyryl-cAMP) revealed differential effects between cells from rats aged 20 and 30 days, implying the involvement of another signalling pathway. Experiments using the specific phosphatidylinositol 3-kinase (PI3-K) inhibitor LY294002 revealed that PI3-K is strongly involved in FSH-induced aromatase expression in Sertoli cells from both 20- and 30-day-old rats. In vivo, this decrease could be explained by a negative effect exerted by germ cells because, in coculture, aromatase gene expression in 20-day-old Sertoli cells is greatly diminished.

Vitamin D Receptor (VDR) Allelic Variants Correlating with Response to Vitamin D3 Supplementation in Breast Cancer Survivors

2021 ◽  
pp. 1-14
Author(s):  
Elham Kazemian ◽  
Sayed Hossein Davoodi ◽  
Mohammad Esmaeil Akbari ◽  
Nariman Moradi ◽  
Safoora Gharibzadeh ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Vitamin D ◽  
Cancer Survivors ◽  
Vitamin D Receptor ◽  
Vitamin D3 ◽  
Breast Cancer Survivors ◽  
Allelic Variants ◽  
Vitamin D3 Supplementation

Identification of a highly potent vitamin D receptor antagonist: (25S)-26-Adamantyl-25-hydroxy-2-methylene-22,23-didehydro-19,27-dinor-20-epi-vitamin D3 (ADMI3)

2007 ◽  
Vol 460 (2) ◽  
pp. 240-253 ◽  
Author(s):  
Miharu Igarashi ◽  
Nobuko Yoshimoto ◽  
Keiko Yamamoto ◽  
Masato Shimizu ◽  
Michiyasu Ishizawa ◽  
...  
Keyword(s):  
Vitamin D ◽  
Receptor Antagonist ◽  
Vitamin D Receptor ◽  
Vitamin D3

scholarly journals A Positive GATA Element and a Negative Vitamin D Receptor-Like Element Control Atrial Chamber-Specific Expression of a Slow Myosin Heavy-Chain Gene during Cardiac Morphogenesis

1998 ◽  
Vol 18 (10) ◽  
pp. 6023-6034 ◽  
Author(s):  
Gang Feng Wang ◽  
William Nikovits ◽  
Mark Schleinitz ◽  
Frank E. Stockdale
Keyword(s):  
Gene Expression ◽  
Vitamin D ◽  
Vitamin D Receptor ◽  
Heavy Chain ◽  
Inhibitory Activity ◽  
Binding Motif ◽  
Specific Expression ◽  
Ventricular Cardiomyocytes ◽  
Slow Myosin Heavy Chain ◽  
Slow Myhc

ABSTRACT We have used the slow myosin heavy chain (MyHC) 3 gene to study the molecular mechanisms that control atrial chamber-specific gene expression. Initially, slow MyHC 3 is uniformly expressed throughout the tubular heart of the quail embryo. As cardiac development proceeds, an anterior-posterior gradient of slow MyHC 3 expression develops, culminating in atrial chamber-restricted expression of this gene following chamberization. Two cis elements within the slow MyHC 3 gene promoter, a GATA-binding motif and a vitamin D receptor (VDR)-like binding motif, control chamber-specific expression. The GATA element of the slow MyHC 3 is sufficient for expression of a heterologous reporter gene in both atrial and ventricular cardiomyocytes, and expression of GATA-4, but not Nkx2-5 or myocyte enhancer factor 2C, activates reporter gene expression in fibroblasts. Equivalent levels of GATA-binding activity were found in extracts of atrial and ventricular cardiomyocytes from embryonic chamberized hearts. These observations suggest that GATA factors positively regulate slow MyHC 3 gene expression throughout the tubular heart and subsequently in the atria. In contrast, an inhibitory activity, operating through the VDR-like element, increased in ventricular cardiomyocytes during the transition of the heart from a tubular to a chambered structure. Overexpression of the VDR, acting via the VDR-like element, duplicates the inhibitory activity in ventricular but not in atrial cardiomyocytes. These data suggest that atrial chamber-specific expression of the slow MyHC 3 gene is achieved through the VDR-like inhibitory element in ventricular cardiomyocytes at the time distinct atrial and ventricular chambers form.

scholarly journals Presence of gene expression of vitamin D receptor and 24-hydroxylase in OK cells

FEBS Letters ◽  
1994 ◽  
Vol 337 (1) ◽  
pp. 48-51 ◽  
Author(s):  
Eiji Ishimura ◽  
Shigeichi Shoji ◽  
Hidenori Koyama ◽  
Masaaki Inaba ◽  
Yoshiki Nishizawa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Vitamin D ◽  
Vitamin D Receptor ◽  
Ok Cells
Sign in / Sign up

Export Citation Format

Share Document