129 EFFECT OF INSULIN-LIKE GROWTH FACTOR 1 AND FOLLICLE-STIMULATING HORMONE IN A DYNAMIC MEDIUM ON VIABILITY AND FOLLICULAR DEVELOPMENT OF CAPRINE EARLY PREANTRAL FOLLICLES

2012 ◽  
Vol 24 (1) ◽  
pp. 177
Author(s):  
D. Magalhães-Padilha ◽  
F. Gabriela ◽  
K. Haag ◽  
M. Gastal ◽  
K. Jones ◽  
...  
Keyword(s):  
Growth Factor ◽  
Early Stage ◽  
Follicle Stimulating Hormone ◽  
Follicular Development ◽  
Blue Dye ◽  
Insulin Like Growth Factor ◽  
Preantral Follicles ◽  
Secondary Follicle

The goal of this study was to investigate the effect of a dynamic medium supplemented with insulin-like growth factor 1 (IGF-1), FSH, or both on the viability, activation and secondary follicle rates and on the follicle and oocyte diameters of caprine preantral follicles submitted to a long-term (16 days of culture) in vitro culture system. Fragments from goat ovaries (n = 16) obtained from slaughterhouse were cultured in α-MEM containing or not containing IGF-1 (50 ng mL–1), FSH (50 ng mL–1), or both as a dynamic medium that was added in the first (Day 0 to 8) and second (Day 8 to 16) halves of culture, resulting in 6 treatments: α-MEM alone, IGF-1/IGF-1, FSH/FSH, IGF-1/FSH, FSH/IGF-1 and IGF-1 + FSH/IGF-1 + FSH. Noncultured (fresh control) and cultured fragments were processed for morphological and viability analyses. Follicles within ovarian fragments were mechanically isolated and early-stage follicles were classified as normal or abnormal and primordial, primary, or secondary. The viability of isolated follicles was determined by trypan blue dye and follicles were classified as live or dead. For this experiment, 6240 preantral follicles were analysed. Data for statistical analyses were transformed and submitted to ANOVA using the GLM procedure of SAS, followed by the Duncan test for comparison of means. At Day 8 of culture, more (P < 0.05) follicles in the treatments containing IGF-1 alone or associated with FSH were normal and viable than in the treatments cultured with FSH or α-MEM alone. At Day 16 of culture, the highest (P < 0.05) percentage of viability was observed in the IGF-1/IGF-1 (68%), IGF-1/FSH (68%) and IGF-1 + FSH/IGF-1 + FSH (72%) treatments. However, more (P < 0.05) normal follicles were observed on Day 16 in the IGF-1 + FSH/IGF-1 + FSH treatment (76%) than in all other treatments, except for the IGF-1/FSH treatment (72%). The percentage of follicular activation (primordial to primary) increased (P < 0.05) in all treatments from Day 0 to 8 (mean, 5 to 49%, respectively). The rate of follicular activation increased (P < 0.05) from Day 8 to 16 in all groups, except for the FSH/FSH and IGF-1 + FSH/IGF-1 + FSH treatments. Nevertheless, at Day 16, the IGF-1 + FSH/IGF-1 + FSH treatment had the highest (P < 0.05) percentage of secondary follicles (28%) when compared with the other groups (range, 6 to 17%). Furthermore, the IGF-1 + FSH/IGF-1 + FSH treatment had the largest (P < 0.05) mean follicular and oocyte diameters after 16 days of culture. In summary, follicular morphology and viability were maintained, follicle activation was promoted and secondary follicle formation was stimulated with the association of IGF-1 and FSH. Therefore, our results demonstrate the importance of IGF-1 associated with FSH during the entire long-term culture period on early folliculogenesis in goats.

scholarly journals Association between insulin-like growth factor-I, thyroxine and follicle stimulating hormone on the survival and in vitro development of caprine preantral follicles

2014 ◽  
Vol 21 (2) ◽  
pp. 110-116 ◽  
Author(s):  
Sanely Lourenço da Costa ◽  
Eduardo Paulino da Costa ◽  
Emílio César Martins Pereira ◽  
Wagner Gonzaga Gonçalves ◽  
Talita Fernandes da Silva ◽  
...  
Keyword(s):  
Growth Factor ◽  
Follicle Stimulating Hormone ◽  
Insulin Like Growth Factor ◽  
In Vitro Development ◽  
Preantral Follicles ◽  
Factor I ◽  
Growth Factor I ◽  
Vitro Development ◽  
Stimulating Hormone

scholarly journals Kit ligand and insulin-like growth factor I affect the in vitro development of ovine preantral follicles

2013 ◽  
Vol 115 (1-3) ◽  
pp. 99-102 ◽  
Author(s):  
V.B. Luz ◽  
V.R. Araújo ◽  
A.B.G. Duarte ◽  
G.M. Silva ◽  
R.N. Chaves ◽  
...  
Keyword(s):  
Growth Factor ◽  
Insulin Like Growth Factor ◽  
In Vitro Development ◽  
Preantral Follicles ◽  
Factor I ◽  
Kit Ligand ◽  
Growth Factor I ◽  
Vitro Development

Effects of IAA in combination with FSH on in vitro culture of ovine preantral follicles

Zygote ◽  
2009 ◽  
Vol 18 (1) ◽  
pp. 89-92 ◽  
Author(s):  
Sonia H.F. Costa ◽  
Regiane R. Santos ◽  
Davide Rondina ◽  
Evelyn R. Andrade ◽  
Otávio M. Ohashi ◽  
...  
Keyword(s):  
Trypan Blue ◽  
Follicle Stimulating Hormone ◽  
Follicular Development ◽  
Free Medium ◽  
Minimum Essential Medium ◽  
Viability Test ◽  
Preantral Follicles ◽  
High Concentrations ◽  
Indole 3 Acetic Acid

SummaryOvarian cortical fragments from five adult ewes were in vitro cultured for 1, 3 or 5 days in the presence of minimum essential medium either supplemented or not by follicle-stimulating hormone (FSH) (100 ng/ml) or indole-3-acetic acid (IAA) (10, 20, 40 or 100 ng/ml), alone or in combination. After in vitro culture, ovarian fragments were submitted to follicular isolation and viability test was performed using trypan blue. Addition of IAA (10 ng/ml) to a free-FSH medium resulted in the highest percentages of viable follicles, but was progressively deleterious in higher concentrations (20, 40 and 100 ng/ml) if in absence of FSH. Follicular development was observed only when FSH was added to an IAA-free medium. In conclusion, IAA at a concentration of 10 ng/ml increases follicular survival in vitro. However, at high concentrations (20, 40 or 100 ng/ml), this auxin may be deleterious to preantral follicles, the addition of FSH to the medium being necessary.

scholarly journals Insulin-like growth factor (IGF) system in the oocyte and somatic cells of bovine preantral follicles

Reproduction ◽  
2002 ◽  
pp. 789-797 ◽  
Author(s):  
DG Armstrong ◽  
G Baxter ◽  
CO Hogg ◽  
KJ Woad
Keyword(s):  
Growth Factor ◽  
Granulosa Cells ◽  
Early Stage ◽  
Ovarian Tissue ◽  
Insulin Like Growth Factor ◽  
Preantral Follicles ◽  
Igf System ◽  
Stage Of Development ◽  
Igf Receptor

Many studies have highlighted the role of the insulin-like growth factor (IGF) system in the control of antral follicular growth. However, much less is known about the involvement of the IGF system in the regulation of preantral follicular development. In an attempt to address this lack of knowledge, the present study describes the spatial and temporal patterns of expression of mRNA encoding components of the IGF system in bovine follicles during preantral stages of development. mRNA was detected by in situ hybridization using frozen sections (14 microm) of bovine ovarian tissue. Serial sections were probed with 35S-labelled bovine riboprobes. Type 1 IGF receptor mRNA was detected in granulosa cells and in the oocyte of preantral follicles; however, in this study, as in previous studies, it was not possible to detect mRNA encoding either IGF-I or -II. IGF binding protein (IGFBP)-2 mRNA was present in granulosa cells and oocytes of preantral follicles, and immunoreactive IGFBP-2 was detected around granulosa cells during this early stage of development. Occasionally, preantral follicles were identified in which there was no expression of IGFBP-2 in granulosa cells or the oocyte. IGFBP-3 mRNA was detected in the oocyte of preantral follicles and in the surrounding stromal tissue. mRNAs encoding IGFBP-2 and -3, and type 1 IGF receptor were first detected in type 2 follicles. In conclusion, although the IGF ligands are not expressed in preantral follicles, mRNAs encoding the type 1 IGF receptor, and IGFBP-2 and -3 were present and showed unique spatial patterns of expression within preantral follicles.

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