48 Bisphenols modulate the anti-Müllerian hormone system in bovine granulosa cells cultured in vitro

2022 ◽  
Vol 34 (2) ◽  
pp. 259
Author(s):  
R. H. Patel ◽  
V. B. Truong ◽  
R. Sabry ◽  
K. McCahill ◽  
L. A. Favetta
Keyword(s):  
Granulosa Cells ◽  
Hormone System ◽  
Cells Cultured

On the Structural Changes of Granulosa Cells Cultured in vitro

1981 ◽  
Vol 110 (2) ◽  
pp. 136-145 ◽  
Author(s):  
G.C. Balboni ◽  
S. Zecchi
Keyword(s):  
Granulosa Cells ◽  
Structural Changes ◽  
Cells Cultured

Effect of noradrenaline and dopamine on progesterone and estradiol secretion of human granulosa cells

1993 ◽  
Vol 129 (2) ◽  
pp. 165-168 ◽  
Author(s):  
József Bódis ◽  
Hans R Tinneberg ◽  
Attila Török ◽  
Philippe Cledon ◽  
Volker Hanf ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Direct Action ◽  
Serum Free Medium ◽  
Free Medium ◽  
Vitro Fertilization ◽  
Serum Free ◽  
Human Granulosa Cells ◽  
Estradiol Secretion ◽  
Cells Cultured

The aim of this study was to explore the direct action of noradrenaline and dopamine on progesterone and estradiol secretion of human granulosa cells cultured in serum-free medium. Progesterone and estradiol production was measured in the presence and absence of noradrenaline, dopamine or propranolol using radioimmunoassays; statistical analysis was performed by analysis of variance and Newman-Keul's multiple range test. Twenty-six women aged 31±3 years undergoing in vitro fertilization and embryo transfer for infertility treatment at University Women's Hospital, University of Tübingen, Germany, took part in this study. Noradrenaline significantly inhibited progesterone production by human granulosa cells in a dose-related manner at a concentration of 10−4–10−6 mol/l. Dopamine significantly stimulated estradiol secretion by granulosa cells in an inverse dose-related manner. Both effects were blocked by propranolol. The results suggest that catecholaminergic actions switch over the steroid production of human granulosa cells cultured in serum-free medium from progesterone to estradiol.

Identification of the JY‐1 in sika deer and its effects on granulosa cells cultured in vitro

2019 ◽  
Vol 54 (11) ◽  
pp. 1437-1442
Author(s):  
Xiumin Chen ◽  
Mingjie Xing ◽  
Yifeng Yang ◽  
Min Chen ◽  
Tong Chang ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Sika Deer ◽  
Cells Cultured

Formation of 4-oestrene-3,17-dione (19-norandrostenedione) by porcine granulosa cells in vitro is inhibited by the aromatase inhibitor 4-hydroxyandrostenedione and the cytochrome P-450 inhibitors aminoglutethimide phosphate and ketoconazole

1989 ◽  
Vol 120 (2) ◽  
pp. 251-260 ◽  
Author(s):  
M. W. Khalil ◽  
P. Morley ◽  
M. A. Glasier ◽  
D. T. Armstrong ◽  
T. Lang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Aromatase Inhibitor ◽  
Granulosa Cells ◽  
High Performance ◽  
Reverse Phase ◽  
Porcine Granulosa Cells ◽  
Cytochrome P 450 ◽  
Ovarian Follicular Fluid ◽  
Cells Cultured

ABSTRACT The origin and biosynthesis of 4-oestrene-3,17-dione (19-norandrostenedione), a major steroid in porcine ovarian follicular fluid, was investigated by culturing granulosa cells from 4–6 mm follicles of prepubertal gilts with radiolabelled androstenedione and 19-hydroxyandrostenedione. Steroid metabolites were purified by solvent extraction and lipophilic column chromatography, and analysed by C18 reverse-phase high-performance liquid chromatography. 19-Hydroxyandrostenedione, 19-norandrostenedione and oestradiol-17β were obtained as major metabolites from androstenedione, while 19-norandrostenedione and oestradiol-17β were the major products from 19-hydroxyandrostenedione. Serum alone or serum plus FSH significantly enhanced formation of 19-norandrostenedione and oestradiol-17β from each substrate, compared with controls. Micromolar concentrations (1 μmol/l) of 4-hydroxyandrostenedione, an aromatase inhibitor, significantly reduced formation of 19-norandrostenedione and oestradiol-17β by granulosa cells cultured with serum and FSH. Formation of 19-norandrostenedione and oestradiol-17β from androstenedione and 19-hydroxyandrostenedione was also significantly inhibited by aminoglutethimide phosphate, a cytochrome P-450 inhibitor known to block the conversion of androstenedione to oestrogens. Ketoconazole, an inhibitor of the cytochrome P-450 dependent 17,20-lysase, blocked formation of 19-norandrostenedione and oestradiol-17β only at millimolar concentrations. These results suggest that 19-norsteroid and oestrogen formation from C19 aromatizable androgens may share a common or overlapping pathway, and imply that 19-norsteroid and oestrogen synthesis is mediated by cytochrome P-450 dependent enzymes. Journal of Endocrinology (1989) 120, 251–260

Gonadotrophic regulation of prolactin mediated progesterone secretion in vitro

1985 ◽  
Vol 110 (2) ◽  
pp. 251-256 ◽  
Author(s):  
J. Steven Alexander ◽  
Thomas M. Crisp
Keyword(s):  
Granulosa Cells ◽  
In Vitro Model ◽  
Fold Increase ◽  
Model System ◽  
Regulatory Mechanisms ◽  
Progesterone Secretion ◽  
Vitro Model ◽  
Dose Dependent ◽  
Cells Cultured

Abstract. The effects of preincubating rat granulosa cells with FSH, LH, and Prl on subsequent Prl mediated progesterone secretion were investigated. Granulosa cells were isolated from ovarian follicles 50 h after injection of 5 IU PMSG and were then plated on poly-l-lysine coated coverslips in serum supplemented medium. Cells were preincubated for 24 h in the absence of hormones (control) or with the addition of either 0.25, 2.5, 25 ng/ml rat FSH or rat LH, or 1 μg/ml rat Prl. Following the preincubation period, cells were maintained for an additional 6 or 8 days in the presence or absence of 1 μg/ml Prl. When cells were preincubated with FSH or LH, only the two higher concentrations (2.5 and 25 ng/ml) stimulated significantly more progesterone secretion than control cultures during the 24 h preincubation period. For each series of preincubations, cells cultured for 6 or 8 days in the presence of Prl secreted significantly more progesterone at each day of culture than cells cultured without Prl. Cells preincubated and cultured with Prl secreted only 3–7-fold more progesterone than cells preincubated in control medium and then cultured with Prl. Preincubation with FSH or LH promoted a 20–45-fold increase in Prl mediated progesterone secretion compared to control preincubation cultures that also subsequently were cultured with Prl. The magnitude of Prl mediated progesterone secretion observed through 6 days of culturing was dose dependent on the preincubation concentration of FSH or LH. The establishment of an in vitro model system in which gonadotrophins enhance the responsiveness of granulosa cells to Prl in serum supplemented medium provides the opportunity for study of the regulatory mechanisms involved with the induction and maintenance of such responsiveness.

The effects of phospholipase C on oestradiol and progesterone secretion in porcine granulosa cells cultured in vitro

2019 ◽  
Vol 54 (9) ◽  
pp. 1236-1243 ◽  
Author(s):  
Huali Chen ◽  
Youfu Yang ◽  
Youlin Wang ◽  
Yamei He ◽  
Jiaxin Duan ◽  
...  
Keyword(s):  
Phospholipase C ◽  
Granulosa Cells ◽  
Progesterone Secretion ◽  
Porcine Granulosa Cells ◽  
Cells Cultured

Effect of α-tocopherol on in vitro maturation of bovine cumulus-oocyte complexes

2008 ◽  
Vol 88 (3) ◽  
pp. 463-467 ◽  
Author(s):  
A. Marques ◽  
P. Santos ◽  
G. Antunes ◽  
A. Chaveiro ◽  
F. Moreira da Silva
Keyword(s):  
Granulosa Cells ◽  
In Vitro Maturation ◽  
Detrimental Effect ◽  
Cumulus Cells ◽  
Bovine Oocyte ◽  
Slight Improvement ◽  
Maturation In Vitro ◽  
Bovine Oocytes ◽  
Cells Cultured

This study determined: the effects of α-tocopherol on apoptotic and necrotic levels of cumulus cells after in vitro maturation of bovine oocytes; whether exposure to α-tocopherol facilitates the development of bovine enclosed oocytes to metaphase II; and the effects of this antioxidant on apoptotic and necrotic levels of granulosa cells cultured in vitro. In conclusion, supplementation with α-tocopherol on in vitro maturation of bovine oocytes has a detrimental effect on the ability of oocytes to reach metaphase II, increasing the number of apoptotic and necrotic cumulus cells of bovine cumulus oocyte complexes (COC). This antioxidant showed a slight improvement in the viability of cultured granulosa cells at a concentration of 100 µM. Key words: Bovine, oocyte maturation in vitro, antioxidant, α-tocopherol

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