Scanning electron microscopy of the operculum of Garra lamta (Hamilton) (Cyprinidae:Cypriniformes), an Indian hill stream fish

2010 ◽  
Vol 58 (3) ◽  
pp. 182 ◽  
Author(s):  
Swati Mittal ◽  
Usha Kumari ◽  
Pinky Tripathi ◽  
Ajay Kumar Mittal

The surface architecture of the epidermis on the outer surface of the operculum (OE) and the epithelium on the inner surface of the operculum (EISO) of Garra lamta was examined by scanning electron microscopy. The surface appeared smooth on the OE and wavy on the EISO. A wavy epithelium is considered to facilitate an increase in its stretchability, during the expansion of the branchial chamber. The OE and the EISO were covered by a mosaic pavement of epithelial cells with characteristic patterns of microridges and microbridges. Interspersed between the epithelial cells were mucous goblet cell pores, which were not significantly different in number in the OE and the EISO. Nevertheless, their surface area in the EISO was significantly higher than in the OE. This could be an adaptation to secrete higher amounts of mucus on the EISO for keeping the branchial chamber lining clean, avoiding clogging, the increased slipperiness reducing friction from water flow and increased efficiency in protecting against microbial attachments. Rounded bulges on the OE and the EISO were associated with mucous goblet cells. The absence of the taste buds in the EISO, in contrast to the OE, suggests that their function in the branchial chamber may not be of much significance in this fish. Breeding tubercles on the OE are believed to facilitate better contact between the male and female during breeding.

2021 ◽  
Vol 26 (1) ◽  
pp. 109-117
Author(s):  
H. M. Al-Hyali ◽  
A. Al-Jumily

In this study fowl poxvirus has been isolated from naturally infected birds. This virus induced respiratory distress, 2, 3 and 4 days post inoculation, in intratracheally inoculated 8 week old chickens, these respiratory distress include congestion of tracheal mucosa, increased tracheal secreation and whitish-yellowish pastules appeared at ninth day post inoculation in the upper part of the trachea forming diphtheritic or pseudodiphtheritic membrane. Using scanning electron microscopy to study the changes that occurs in the epithelial surface of trachea. It was found that the virus causes proliferation of epithelial cells forming cluster like papilloma, deciliation and activation of goblet cells.


2018 ◽  
Vol 27 (2) ◽  
pp. 154-160
Author(s):  
David Fernandez Conga ◽  
Pedro Mayor ◽  
Adriano Penha Furtado ◽  
Elane Guerreiro Giese ◽  
Jeannie Nascimento dos Santos

Abstract Dipetalonema gracile (Rudolphi, 1809) (Filarioidea: Onchocercidae) is one of six species of cavities filarial parasites of Neotropical non-human primates. The present study recorded the occurrence of D. gracile, provides morphological and morphometric data and extends the geographical distribution. Adult filariae were obtained from the thoracic and abdominal cavities of 38 specimens of woolly monkey, which were used for local human consumption, in the northeastern Peruvian Amazon. Male and female filarids were processed and analysed using light and scanning electron microscopy. Details of the cephalic papillae, post-cloacal bands and papillae, vulva, phasmid position and lateral appendages are showed by scanning electron microscopy and is recorded the occurrencce of Lagothrix poeppigii monkey as a new host of this filaria in the Yavari-Mirin river basin, Peruvian Amazon.


2018 ◽  
Vol 27 (2) ◽  
pp. 146-153
Author(s):  
Anangela Ravena da Silva Leal ◽  
Simone Mousinho Freire ◽  
Marcelo Knoff ◽  
Delir Corrêa Gomes ◽  
Jeannie Nascimento dos Santos ◽  
...  

Abstract Atractis thapari Petter, 1966, an atractid nematode, was collected parasitizing the large intestine of tortoises of the species Chelonoidis carbonarius (Spix, 1824) (Cc) and C. denticulatus (Linnaeus, 1766) (Cd) (Testudinidae) in the Zoobotanical Park of the municipality of Teresina, state of Piauí, Brazil. Taxonomic identification was based on morphological and morphometrical features, and using bright-field and scanning electron microscopy. The present study adds new observations on the morphology of A. thapari, mainly relating to mouth papillae, the excretory pore, deirids, and male and female posterior ends. The parasitic indices of prevalence (P), mean intensity (MI), mean abundance (MA) and range of infection (RI) for A. thapari in these two tortoise species were: P = 100%, MI = 154,667, MA = 154,667, RI = 5,500-588,500 (Cc); P = 100%, MI = 93,639, MA = 93,639, RI = 1,000-224,500 (Cd). This report confirms the occurrence of A. thapari in Neotropical region, South America, Brazil, and extends its occurrences to a new host, the tortoise C. carbonarius. Adjustment of host management with the aim of improving hygiene and health conditions is suggested.


1988 ◽  
Vol 102 (12) ◽  
pp. 1102-1106 ◽  
Author(s):  
M. Gulisano ◽  
G. Polli ◽  
G. Biondi ◽  
P. Pacini

AbstractTo date only few and often disagreeing studies about human nasopharynx are available.The present research has the purpose to give a contribution to the knowledge of nasopharyngeal epithelium using S.E.M The study was carried out on biopsies taken from 20 healthy volunteers.The surface of nasopharynx is covered by ciliated cells, microvilli provided cells and goblet cells.Areas covered with squamous epithelium and presumably transitional epithelium were observed.The possibility that ciliated become microvilli provided cells is discussed.


IAWA Journal ◽  
1991 ◽  
Vol 12 (4) ◽  
pp. 431-438 ◽  
Author(s):  
Hisashi Abe ◽  
Jun Ohtani ◽  
Kazumi Fukazawa

Field emission scanning electron microscopy was used to observe the inner surfaces of the developing secondary walls of earlywood tracheids of Abies sachalinensis Masters. Microfibrillar orientation in the secondary wall, as seen from the lumen side, changed in a clockwise direction from the outermost S1 to the middle of the S2 and from there counter-clockwise to the innermost S3. Sometimes microfibrils oriented in a steep S-helix were observed in the S3 layer. Lamellae showing different microfibrillar orientations in wall layers other than the S2 were observed beneath newly deposited microfibrils on the inner surface of the developing wall. Furthermore, on the inner surface of the wall forming the S12, S23 and S3, lamellae with microfibrils closely aligned at the same angle as one another and lacking spaces were not observed. These observations suggest that in layers other than the S2 most lamellae are not composed of closely spaced microfibrils.


Parasitology ◽  
1986 ◽  
Vol 93 (3) ◽  
pp. 433-442 ◽  
Author(s):  
V. R. Southgate ◽  
D. Rollinson ◽  
J. Vercruysse

SUMMARYScanning electron microscopical studies of adult male and female Schistosoma curassoni, male S. haematobium and male S. bovis are reported. The tubercles on the dorsal and dorso-lateral surfaces of male S. bovis are devoid of spines, whereas spines are present on the tubercles of male S. curassoni and S. haematobium. Some of the tubercles of S. curassoni and S. haematobium are completely covered in spines, whereas others have a spine-free apical area. Thus, on the isolates examined from Senegal it is concluded that adult male S. bovis can be easily distinguished from S. curassoni or S. haematobium on the basis of the structure of the tubercles. The surface of female S. curassoni is smooth, with transverse striations and a pitted appearance, apart from the posterior part where anteriorly pointing spines are found.


2018 ◽  
Vol 48 (2) ◽  
pp. 142-155 ◽  
Author(s):  
Zofia Dubicka ◽  
Krzysztof Owocki ◽  
Michał Gloc

Abstract The test structures of Lagenida, Rotaliida, and Miliolida (Foraminifera) are described at an unprecedented scale of resolution. Observations using conventional and field-emission scanning electron microscopy revealed distinct micro- and nanoscale differences in the textural compositions of these three main groups of calcifying foraminifers, consistent with recent molecular phylogenetic reconstructions and a higher-level taxonomic system. The rotaliid test is entirely composed of roughly spherical primary carbonate nanograins, up to 100 nm in diameter, which merge into micrometer-sized irregular aggregates. The miliolid test is made up of two morphologically different primary crystallites. Arbitrarily arranged needle-shaped elements (up to 1 µm in length and 200 nm in width) make up the bulk of the test, including the inside of the wall (porcelain) and mineralized inner surface (intrados) (ca. 100 nm in thickness). Roughly spherical nanograins (up to 50 nm in diameter) form more or less regularly arranged polygons of an outer lamina (extrados), which is ca. 200 nm in thickness. By contrast, the lagenid test texture is characterized by much larger crystals than in other calcifying foraminifers. At moderate magnification, lagenid tests display a fibrous texture composed of fiber bundles (tens of μm in length and several μm in width) that are oriented perpendicular to test surfaces and taper towards the ends when in contact with another lamina. At higher magnification, each bundle constitutes a single calcite crystal with an inner pore extending along the entire length of the crystal/fiber. We measured test hardness using the nanoindentation method. This is the first application of this technique in microfossils. We found that Cretaceous Lagenida tests were more resistant to mechanical stress than Rotaliida tests. These comparative strengths may be linked to internal test microstructure and play a role in determining habitats in which these taxa can live.


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