scholarly journals Biological and immunological characteristics of hepatitis E virus-like particles based on the crystal structure

2009 ◽  
Vol 106 (31) ◽  
pp. 12986-12991 ◽  
Author(s):  
T. Yamashita ◽  
Y. Mori ◽  
N. Miyazaki ◽  
R. H. Cheng ◽  
M. Yoshimura ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Like Particles

scholarly journals Efficient Production of Chimeric Hepatitis B Virus-Like Particles Bearing an Epitope of Hepatitis E Virus Capsid by Transient Expression in Nicotiana benthamiana

Life ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 64
Author(s):  
Gergana Zahmanova ◽  
Milena Mazalovska ◽  
Katerina Takova ◽  
Valentina Toneva ◽  
Ivan Minkov ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Nicotiana Benthamiana ◽  
Hepatitis E Virus ◽  
Chimeric Protein ◽  
Hepatitis E ◽  
Core Antigen ◽  
Efficient Production ◽  
Virus Like Particles ◽  
B Virus

The core antigen of hepatitis B virus (HBcAg) is capable of self-assembly into virus-like particles (VLPs) when expressed in a number of heterologous systems. Such VLPs are potential carriers of foreign antigenic sequences for vaccine design. In this study, we evaluated the production of chimeric HBcAg VLPs presenting a foreign epitope on their surface, the 551–607 amino acids (aa) immunological epitope of the ORF2 capsid protein of hepatitis E virus. A chimeric construct was made by the insertion of 56 aa into the immunodominant loop of the HBcAg. The sequences encoding the chimera were inserted into the pEAQ-HT vector and infiltrated into Nicotiana benthamiana leaves. The plant-expressed chimeric HBcHEV ORF2 551–607 protein was recognized by an anti-HBcAg mAb and anti-HEV IgG positive swine serum. Electron microscopy showed that plant-produced chimeric protein spontaneously assembled into “knobbly” ~34 nm diameter VLPs. This study shows that HBcAg is a promising carrier platform for the neutralizing epitopes of hepatitis E virus (HEV) and the chimeric HBcAg/HEV VLPs could be a candidate for a bivalent vaccine.

scholarly journals Induction of antibody and interferon-γ production in mice immunized with virus-like particles of swine hepatitis E virus

2014 ◽  
Vol 15 (4) ◽  
pp. 575 ◽  
Author(s):  
Young-Jo Song ◽  
Woo-Jung Park ◽  
Seul-Kee Lee ◽  
Joong-Bok Lee ◽  
Seung-Yong Park ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Interferon Γ ◽  
Virus Like Particles ◽  
Swine Hepatitis

scholarly journals Chimeric Recombinant Hepatitis E Virus-like Particles as an Oral Vaccine Vehicle Presenting Foreign Epitopes

Virology ◽  
2002 ◽  
Vol 293 (2) ◽  
pp. 273-280 ◽  
Author(s):  
Masahiro Niikura ◽  
Shiki Takamura ◽  
Gisen Kim ◽  
Satoru Kawai ◽  
Masayuki Saijo ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Oral Vaccine ◽  
Hepatitis E ◽  
Recombinant Hepatitis ◽  
Virus Like Particles

Generation of hepatitis E virus-like particles of two new genotypes G5 and G6 and comparison of antigenic properties with those of known genotypes

2015 ◽  
Vol 178 (1-2) ◽  
pp. 150-157 ◽  
Author(s):  
Tian-Cheng Li ◽  
Michiyo Kataoka ◽  
Kazuaki Takahashi ◽  
Sayaka Yoshizaki ◽  
Takanobu Kato ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Like Particles ◽  
Antigenic Properties

scholarly journals Liver-specific Gene Delivery Using Engineered Virus-Like Particles of Hepatitis E Virus

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Eun Byul Lee ◽  
Jung-Hee Kim ◽  
Wonhee Hur ◽  
Jung Eun Choi ◽  
Sung Min Kim ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Specific Gene ◽  
Virus Like Particles

The use of Bayesian methods for evaluating the performance of a virus-like particles-based ELISA for serology of hepatitis E virus infection in swine

2010 ◽  
Vol 163 (2) ◽  
pp. 329-335 ◽  
Author(s):  
Nicolas Rose ◽  
Annie Boutrouille ◽  
Christelle Fablet ◽  
François Madec ◽  
Marc Eloit ◽  
...  
Keyword(s):  
Virus Infection ◽  
Bayesian Methods ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Like Particles

scholarly journals 910. Evaluation of an Enzymatic Immunoassay System for Detection and Differentiation of Rat Hepatitis E Virus Infection in Humans

2021 ◽  
Vol 8 (Supplement_1) ◽  
pp. S546-S547
Author(s):  
Siddharth Sridhar ◽  
Jianwen Situ ◽  
Kelvin Hon Yin Lo ◽  
Jianpiao Cai
Keyword(s):  
Hepatitis E Virus ◽  
Roc Analysis ◽  
Confusion Matrix ◽  
Diagnostic Testing ◽  
Sequence Divergence ◽  
Hepatitis E ◽  
Human Populations ◽  
Rt Pcr ◽  
Serological Tests ◽  
Virus Like Particles

Abstract Background Previously, hepatitis E in humans was believed to be caused exclusively by species A variants of the Orthohepevirus genus (HEV-A) of the family Hepeviridae. However, we have previously demonstrated that Orthohepevirus species C (HEV-C), also known as rat hepatitis E virus, also causes hepatitis in humans. Due to high sequence divergence between HEV-A and HEV-C, serological tests based on HEV-A are often insensitive for HEV-C diagnosis. Therefore, we developed an enzymatic immunoassay (EIA) system for differentiating HEV-A and HEV-C antibody signatures in patient sera. Methods HEV-A and HEV-C peptide homologs spanning the entire immunogenic E2s region of HEV ORF2 capsid protein were expressed in E. coli. These peptides, HEV-A4 p239 and HEV-C p241, form virus-like particles (figure 1). Both peptides were coated in separate 96-well plates. Sera obtained from patients with RT-PCR confirmed acute HEV-A infection (n = 54), HEV-C infection (n = 15), and uninfected HEV seronegative controls (n = 126) were tested in parallel in HEV-A4 p239 and HEV-C p241 EIAs for respective IgG antibodies. Sample optical densities (ODs) were divided by mean OD + 3SD of the seronegative controls to generate signal/noise (S/N) ratios. S/Ns marking positivity in either assay were determined by ROC analysis. An algorithm for assay interpretation was developed (table 1) and the performance of this algorithm was measured against the RT-PCR gold standard. HEV-A4 p239 and HEV-C1 p241 virus like particles Transmission electron microscopy images of the two peptides used in this study Diagnostic testing algorithm interpretation Interpretation of results of testing using parallel enzymatic immunoassays Results Using cutoffs determined by ROC analysis (figure 2), HEV-A4 p239 and HEV-C p241 EIAs detected species-specific antibody responses well (sensitivity: 92.6% and 80% respectively) and were specific (92.9% and 98.3% respectively). The DC was 100% congruent with HEV-C RT-PCR and 88.9% congruent with HEV-A RT-PCR in RT-PCR positive samples. Incorporating all three cutoffs into the algorithm, we derived a 3×3 confusion matrix of RT-PCR sample assignation vs EIA algorithm classification (table 2). The Cohen’s κ value was 0.883 indicating excellent inter-rater reliability. ROC analysis for determining S/N cutoffs Curve for A/A represents analysis for HEV-A4 p239 EIA. Curve for C/¬C represents analysis for HEV-C p241 EIA. Curve for r(C/A) represents analysis for the differentiating ratio. 3×3 confusion matrix comparing sample assignations by RT-PCR vs. EIA algorithm Conclusion A parallel EIA system accurately differentiated HEV-A and HEV-C serological signatures in acute patient sera. This method can now be applied to seroprevalence studies to determine seroprevalence of rat hepatitis E in human populations. Disclosures Siddharth Sridhar, FRCPath, Abbott (Other Financial or Material Support, Speaker’s honoraria)

Expression and self-assembly of empty virus-like particles of hepatitis E virus.

1997 ◽  
Vol 71 (10) ◽  
pp. 7207-7213 ◽  
Author(s):  
T C Li ◽  
Y Yamakawa ◽  
K Suzuki ◽  
M Tatsumi ◽  
M A Razak ◽  
...  
Keyword(s):  
Self Assembly ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Like Particles

scholarly journals Characterization of self-assembled virus-like particles of ferret hepatitis E virus generated by recombinant baculoviruses

2013 ◽  
Vol 94 (12) ◽  
pp. 2647-2656 ◽  
Author(s):  
Tingting Yang ◽  
Michiyo Kataoka ◽  
Yasushi Ami ◽  
Yuriko Suzaki ◽  
Noriko Kishida ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Expression System ◽  
Recombinant Baculovirus ◽  
Hepatitis E ◽  
Electron Microscopic ◽  
Antigenic Analysis ◽  
Virus Like Particles ◽  
C Terminus ◽  
N Terminus ◽  
Self Assembled

Ferret hepatitis E virus (HEV), a novel hepatitis E-like virus, has been identified in ferrets in The Netherlands. Due to the lack of a cell-culture system for ferret HEV, the antigenicity, pathogenicity and epidemiology of this virus have remained unclear. In the present study, we used a recombinant baculovirus expression system to express the 112-N-terminus and 47-C-terminus-amino-acid-truncated ferret HEV ORF2 protein in insect Tn5 cells, and found that a large amount of a 53 kDa protein (F-p53) was expressed and efficiently released into the supernatant. Electron microscopic analysis revealed that F-p53 was self-assembled into virus-like particles (ferret HEV-LPs). These ferret HEV-LPs were estimated to be 24 nm in diameter, which is similar to the size of G1, G3, G4 and rat HEV-LPs derived from both the N-terminus- and C-terminus-truncated constructs. Antigenic analysis demonstrated that ferret HEV-LPs were cross-reactive with G1, G3, G4 and rat HEVs, and rat HEV and ferret HEV showed a stronger cross-reactivity to each other than either did to human HEV genotypes. However, the antibody against ferret HEV-LPs does not neutralize G3 HEV, suggesting that the serotypes of these two HEVs are different. An ELISA for detection of anti-ferret HEV IgG and IgM antibodies was established using ferret HEV-LPs as antigen, and this assay system will be useful for monitoring ferret HEV infection in ferrets as well as other animals. In addition, analysis of ferret HEV RNA detected in ferret sera collected from a breeding colony in the USA revealed the genetic diversity of ferret HEV.

Sign in / Sign up

Export Citation Format

Share Document