scholarly journals AGLF provides C-function in floral organ identity through transcriptional regulation of AGAMOUS in Medicago truncatula

2019 ◽  
Vol 116 (11) ◽  
pp. 5176-5181 ◽  
Author(s):  
Yang Zhao ◽  
Rong Liu ◽  
Yiteng Xu ◽  
Minmin Wang ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Flower Development ◽  
Molecular Mechanisms ◽  
Floral Organ ◽  
Model Systems ◽  
Loss Of Function ◽  
Model Legume ◽  
Floral Organ Identity ◽  
Organ Identity ◽  
Class B

Floral development is one of the model systems for investigating the mechanisms underlying organogenesis in plants. Floral organ identity is controlled by the well-known ABC model, which has been generalized to many flowering plants. Here, we report a previously uncharacterized MYB-like gene, AGAMOUS-LIKE FLOWER (AGLF), involved in flower development in the model legume Medicago truncatula. Loss-of-function of AGLF results in flowers with stamens and carpel transformed into extra whorls of petals and sepals. Compared with the loss-of-function mutant of the class C gene AGAMOUS (MtAG) in M. truncatula, the defects in floral organ identity are similar between aglf and mtag, but the floral indeterminacy is enhanced in the aglf mutant. Knockout of AGLF in the mutants of the class A gene MtAP1 or the class B gene MtPI leads to an addition of a loss-of-C-function phenotype, reflecting a conventional relationship of AGLF with the canonical A and B genes. Furthermore, we demonstrate that AGLF activates MtAG in transcriptional levels in control of floral organ identity. These data shed light on the conserved and diverged molecular mechanisms that control flower development and morphology among plant species.

scholarly journals Transcriptome-Wide Analyses Provide Insights into Development of the Hedychium Coronarium Flower, Revealing Potential Roles of PTL

2020 ◽  
Author(s):  
Tong Zhao ◽  
Alma Piñeyro-Nelson ◽  
Qianxia Yu ◽  
Xiaoying Hu ◽  
Huanfang Liu ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Flower Development ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Floral Organ ◽  
Mrna In Situ Hybridization ◽  
Hedychium Coronarium ◽  
Organ Identity

Abstract Background:The flower of Hedychium coronarium possesses highly specialized floral organs: a synsepalous calyx, petaloid staminodes and a labellum. The formation of these organs is controlled by two gene categories: floral organ identity genes and organ boundary genes, which may function individually or jointly during flower development. Although the floral organogenesis of H. coronarium has been studied at the morphological level, the underlying molecular mechanisms involved in its floral development still remain poorly understood. In addition, previous works analyzing the role of MADS-box genes in controlling floral organ specification in some Zingiberaceae did not address the molecular mechanisms involved in the formation of particular organ morphologies that emerge later in flower development, such as the synsepalous calyx formed through intercalary growth of adjacent sepals. Results:Here, we used comparative transcriptomics combined with Real-time quantitative PCR and mRNA in situ hybridization to investigate gene expression patterns of ABC-class genes in H. coronarium flowers, as well as the homolog of the organ boundary gene PETAL LOSS (HcPTL). qRT-PCR detection showed that HcAP3 and HcAG were expressed in both the petaloid staminode and the fertile stamen. mRNA in situ hybridization showed that HcPTL was expressed in developing meristems, including cincinnus primordia, floral primordia, common primordia and almost all new initiating floral organ primordia.Conclusions:Our studies found that stamen/petal identity or stamen fertility in H. coronarium was not necessarily correlated with the differential expression of HcAP3 and HcAG. We also found a novel spatio-temporal expression pattern for HcPTL mRNA, suggesting it may have evolved a lineage-specific role in the morphogenesis of the Hedychium flower. Our study provides a new transcriptome reference and a functional hypothesis regarding the role of a boundary gene in organ fusion that should be further addressed through phylogenetic analyzes of this gene, as well as functional studies.

scholarly journals Divergence of function and regulation of class B floral organ identity genes.

1997 ◽  
Vol 9 (4) ◽  
pp. 559-570 ◽  
Author(s):  
A Samach ◽  
S E Kohalmi ◽  
P Motte ◽  
R Datla ◽  
G W Haughn
Keyword(s):  
Floral Organ ◽  
Floral Organ Identity ◽  
Organ Identity ◽  
Class B ◽  
Floral Organ Identity Genes

scholarly journals Divergence of Function and Regulation of Class B Floral Organ Identity Genes

1997 ◽  
Vol 9 (4) ◽  
pp. 559
Author(s):  
Alon Samach ◽  
Susanne E. Kohalmi ◽  
Patrick Motte ◽  
Raju Datla ◽  
George W. Haughn
Keyword(s):  
Floral Organ ◽  
Floral Organ Identity ◽  
Organ Identity ◽  
Class B ◽  
Floral Organ Identity Genes

scholarly journals A Factor Linking Floral Organ Identity and Growth Revealed by Characterization of the Tomato Mutant unfinished flower development (ufd)

2016 ◽  
Vol 7 ◽  
Author(s):  
Sandra Poyatos-Pertíñez ◽  
Muriel Quinet ◽  
Ana Ortíz-Atienza ◽  
Fernando J. Yuste-Lisbona ◽  
Clara Pons ◽  
...  
Keyword(s):  
Flower Development ◽  
Floral Organ ◽  
Floral Organ Identity ◽  
Organ Identity ◽  
Tomato Mutant

The interaction between MtDELLA-MtGAF1 complex and MtARF3 mediates transcriptional control of MtGA3ox1 to elaborate leaf margin formation in Medicago truncatula

2021 ◽  
Author(s):  
Lizhu Wen ◽  
Yiming Kong ◽  
Hongfeng Wang ◽  
Yiteng Xu ◽  
Zhichao Lu ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Medicago Truncatula ◽  
Transcriptional Control ◽  
Molecular Mechanisms ◽  
Leaf Shape ◽  
Critical Role ◽  
Competitive Inhibitor ◽  
Leaf Margin ◽  
Loss Of Function ◽  
Model Legume

Abstract The molecular mechanisms underlying diversity of leaf shapes have been of great interest to researchers. Leaf shape depends on the pattern of serrations and the degree of indentation of leaf margins. Multiple transcription factors and hormone signaling are involved in this process. In this study, we characterized the developmental roles of SMALL AND SERRATED LEAF (SSL) by analyzing a recessive mutant in the model legume Medicago truncatula. An ortholog of Arabidopsis thaliana GA3-oxidase 1 (GA3ox1), MtGA3ox1/SSL, is required for GA biosynthesis. Loss of function in MtGA3ox1 results in the small plant and lateral organs. The prominent phenotype of the mtga3ox1 mutant is the more pronounced leaf margin, indicating the critical role of GA level in leaf margin formation. Moreover, 35S: MtDELLA2  ΔDELLAand 35S: MtARF3 transgenic plants display leaves with the deeply wavy margin, which resembles those of mtga3ox1. Further investigations show that the MtGA3ox1 is under the control of MtDELLA1/2/3-MtGAF1 complexes-dependent feedback regulation. Meanwhile, MtARF3 behaves as a competitive inhibitor of MtDELLA2/3-MtGAF1 complexes to repress the expression of MtGA3ox1 indirectly. These findings suggest that GA feedback regulatory circuits play a fundamental role in leaf margin formation, in which the posttranslational interaction between transcription factors functions as an additional feature.

MtNAM regulates floral organ identity and lateral organ separation in Medicago truncatula

2019 ◽  
pp. 197-206
Author(s):  
Xiaofei Cheng ◽  
Jianling Peng ◽  
Rujin Chen ◽  
Kirankumar S. Mysore ◽  
Jiangqi Wen
Keyword(s):  
Medicago Truncatula ◽  
Floral Organ ◽  
Floral Organ Identity ◽  
Organ Identity ◽  
Lateral Organ

scholarly journals MIR172d Is Required for Floral Organ Identity and Number in Tomato

2021 ◽  
Vol 22 (9) ◽  
pp. 4659
Author(s):  
Wanping Lin ◽  
Suresh Kumar Gupta ◽  
Tzahi Arazi ◽  
Ben Spitzer-Rimon
Keyword(s):  
Transcription Factors ◽  
Transcriptional Repression ◽  
De Novo ◽  
Floral Organ ◽  
Dependent Manner ◽  
Loss Of Function ◽  
Floral Organ Identity ◽  
Organ Identity ◽  
Unique Species ◽  
Dose Dependent

MicroRNA172 (miR172) functions as a central regulator of flowering time and flower development by post-transcriptional repression of APETALA2-LIKE transcription factors. In the model crop Solanum lycopersicum (tomato), the miR172 family is still poorly annotated and information about the functions of specific members is lacking. Here, de-novo prediction of tomato miR172 coding loci identified seven genes (SlMIR172a-g), that code for four unique species of miR172 (sly-miR172). During reproductive development, sly-miR172s are differentially expressed, with sly-miR172c and sly-miR172d being the most abundant members in developing flowers, and are predicted to guide the cleavage of eight APETALA2-LIKE transcription factors. By CRISPR-Cas9 co-targeting of SlMIR172c and SlMIR172d we have generated a battery of loss-of-function and hypomorphic mutants (slmir172c-dCR). The slmir172c-dCR plants developed normal shoot but their flowers displayed graded floral organ abnormalities. Whereas slmir172cCR loss-of-function caused only a slight greening of petals and stamens, hypomorphic and loss-of-function slmir172dCR alleles were associated with the conversion of petals and stamens to sepaloids, which were produced in excess. Interestingly, the degrees of floral organ identity alteration and proliferation were directly correlated with the reduction in sly-miR172d activity. These results suggest that sly-miR172d regulates in a dose-dependent manner floral organ identity and number, likely by negatively regulating its APETALA2-like targets.

scholarly journals NO APICAL MERISTEM (MtNAM) regulates floral organ identity and lateral organ separation in Medicago truncatula

2012 ◽  
Vol 195 (1) ◽  
pp. 71-84 ◽  
Author(s):  
Xiaofei Cheng ◽  
Jianling Peng ◽  
Junying Ma ◽  
Yuhong Tang ◽  
Rujin Chen ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Apical Meristem ◽  
Floral Organ ◽  
Floral Organ Identity ◽  
Organ Identity ◽  
Lateral Organ
Sign in / Sign up

Export Citation Format

Share Document