scholarly journals Production of transgenic rats by lentiviral transduction of male germ-line stem cells

2002 ◽  
Vol 99 (23) ◽  
pp. 14931-14936 ◽  
Author(s):  
F. K. Hamra ◽  
J. Gatlin ◽  
K. M. Chapman ◽  
D. M. Grellhesl ◽  
J. V. Garcia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Line ◽  
Transgenic Rats ◽  
Male Germ Line ◽  
Lentiviral Transduction

Effect of Silver Nanoparticles on SRC Activity in Male Germ-line Stem Cells

2006 ◽  
Author(s):  
Laura K. Braydich-Stolle ◽  
Saber Hussain ◽  
John J. Schlager ◽  
Marie-Claude Hofmann
Keyword(s):  
Stem Cells ◽  
Silver Nanoparticles ◽  
Germ Line ◽  
Male Germ Line

Male germ line stem cells: from cell biology to cell therapy

2003 ◽  
Vol 15 (6) ◽  
pp. 323 ◽  
Author(s):  
David Pei-Cheng Lin ◽  
Ming-Yu Chang ◽  
Bo-Yie Chen ◽  
Han-Hsin Chang
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Lines ◽  
Germ Cells ◽  
Germ Line ◽  
Current Status ◽  
Seminiferous Tubules ◽  
Male Germ Line ◽  
Male Germ Cells ◽  
Stem Cell Lines

Research using stem cells has several applications in basic biology and clinical medicine. Recent advances in the establishment of male germ line stem cells provided researchers with the ability to identify, isolate, maintain, expand and differentiate the spermatogonia, the primitive male germ cells, as cell lines under in vitro conditions. The ability to culture and manipulate stem cell lines from male germ cells has gradually facilitated research into spermatogenesis and male infertility, to an extent beyond that facilitated by the use of somatic stem cells. After the introduction of exogenous genes, the spermatogonial cells can be transplanted into the seminiferous tubules of recipients, where the transplanted cells can contribute to the offspring. The present review concentrates on the origin, life cycle and establishment of stem cell lines from male germ cells, as well as the current status of transplantation techniques and the application of spermatogonial stem cell lines.

scholarly journals Retrovirus-mediated gene delivery into male germ line stem cells

FEBS Letters ◽  
2000 ◽  
Vol 475 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Makoto Nagano ◽  
Takashi Shinohara ◽  
Mary R Avarbock ◽  
Ralph L Brinster
Keyword(s):  
Stem Cells ◽  
Gene Delivery ◽  
Germ Line ◽  
Male Germ Line

Adeno‐associated virus (AAV)‐mediated transduction of male germ line stem cells results in transgene transmission after germ cell transplantation

2007 ◽  
Vol 22 (2) ◽  
pp. 374-382 ◽  
Author(s):  
Ali Honaramooz ◽  
Susan Megee ◽  
Wenxian Zeng ◽  
Margret M. Destrempes ◽  
Susan A. Overton ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Germ Line ◽  
Male Germ Line ◽  
Adeno Associated Virus ◽  
Germ Cell Transplantation ◽  
Transgene Transmission

scholarly journals Differential Genomic Imprinting and Expression of Imprinted microRNAs in Testes-Derived Male Germ-Line Stem Cells in Mouse

PLoS ONE ◽  
2011 ◽  
Vol 6 (7) ◽  
pp. e22481 ◽  
Author(s):  
Ji Young Shin ◽  
Mukesh Kumar Gupta ◽  
Yoon Hee Jung ◽  
Sang Jun Uhm ◽  
Hoon Taek Lee
Keyword(s):  
Stem Cells ◽  
Genomic Imprinting ◽  
Germ Line ◽  
Male Germ Line

scholarly journals Generation of Transgenic Rats through Induced Pluripotent Stem Cells

2013 ◽  
Vol 288 (38) ◽  
pp. 27150-27158 ◽  
Author(s):  
Ming-Gui Jiang ◽  
Tianda Li ◽  
Chunjing Feng ◽  
Rui Fu ◽  
Yan Yuan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Germ Line ◽  
Glycogen Synthase ◽  
Embryonic Stem ◽  
Mapk Signaling ◽  
Transgenic Rats ◽  
Induced Pluripotent

The rat is an important animal model for human disease research. Using inhibitors of glycogen synthase kinase 3 and MAPK signaling pathways, rat embryonic stem cells and rat induced pluripotent stem cells (riPSCs) have been derived. However, unlike rat embryonic stem cells, germ line competent riPSCs have only been derived from Wistar rats at low efficiency. Here, we found that an optimized induction medium containing knock-out serum replacement and vitamin C improved the rate and efficiency of riPSCs generation from Dark Agouti rat fibroblasts and Sertoli cells. riPSCs maintained an undifferentiated status for >30 passages and could differentiate into various cells types including germ cells when injected into rat blastocysts. Moreover, transgenic riPSCs could be generated through the PiggyBac transposon, which could be used to generate transgenic rats through germ line transmission. riPSCs can be used as a novel tool in genetic and genomic studies of the rat.

Sign in / Sign up

Export Citation Format

Share Document