scholarly journals Introns and intein coding sequence in the ribonucleotide reductase genes of Bacillus subtilis temperate bacteriophage SP 

1998 ◽  
Vol 95 (4) ◽  
pp. 1692-1697 ◽  
Author(s):  
V. Lazarevic ◽  
B. Soldo ◽  
A. Dusterhoft ◽  
H. Hilbert ◽  
C. Mauel ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Ribonucleotide Reductase ◽  
Temperate Bacteriophage ◽  
Coding Sequence

Rational Design of the N-Terminal Coding Sequence for Regulating Enzyme Expression in Bacillus subtilis

2021 ◽  
Vol 10 (2) ◽  
pp. 265-276
Author(s):  
Kuidong Xu ◽  
Yi Tong ◽  
Yi Li ◽  
Jin Tao ◽  
Jianghua Li ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Rational Design ◽  
Enzyme Expression ◽  
Coding Sequence

scholarly journals Tetracycline Induces Stabilization of mRNA in Bacillus subtilis

2002 ◽  
Vol 184 (4) ◽  
pp. 889-894 ◽  
Author(s):  
Yi Wei ◽  
David H. Bechhofer
Keyword(s):  
Bacillus Subtilis ◽  
Mrna Stability ◽  
The Other ◽  
Leader Region ◽  
Subinhibitory Concentration ◽  
Coding Sequence ◽  
Mrna Stabilization ◽  
Threefold Increase ◽  
The Stability

ABSTRACT The tet(L) gene of Bacillus subtilis confers low-level tetracycline (Tc) resistance. Previous work examining the >20-fold-inducible expression of tet(L) by Tc demonstrated a 12-fold translational induction. Here we show that the other component of tet(L) induction is at the level of mRNA stabilization. Addition of a subinhibitory concentration of Tc results in a two- to threefold increase in tet(L) mRNA stability. Using a plasmid-borne derivative of tet(L) with a large in-frame deletion of the coding sequence, the mechanism of Tc-induced stability was explored by measuring the decay of tet(L) mRNAs carrying specific mutations in the leader region. The results of these experiments, as well as experiments with a B. subtilis strain that is resistant to Tc due to a mutation in the ribosomal S10 protein, suggest different mechanisms for the effects of Tc on translation and on mRNA stability. The key role of the 5" end in determining mRNA stability was confirmed in these experiments. Surprisingly, the stability of several other B. subtilis mRNAs was also induced by Tc, which indicates that addition of Tc may result in a general stabilization of mRNA.

LC–MS/MS proteomic analysis of starved Bacillus subtilis cells overexpressing ribonucleotide reductase (nrdEF): implications in stress-associated mutagenesis

2017 ◽  
Vol 64 (1) ◽  
pp. 215-222 ◽  
Author(s):  
Karla Viridiana Castro-Cerritos ◽  
Adolfo Lopez-Torres ◽  
Armando Obregón-Herrera ◽  
Katarzyna Wrobel ◽  
Kazimierz Wrobel ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Ribonucleotide Reductase ◽  
Proteomic Analysis

A novel form of allosteric regulation in Bacillus subtilis ribonucleotide reductase revealed by SAXS and cryo-electron microscopy

2018 ◽  
Vol 74 (a1) ◽  
pp. a85-a85
Author(s):  
William C. Thomas ◽  
Frederick P. Brooks ◽  
Mackenzie J. Parker ◽  
David A. Case ◽  
Jason T. Kaelber ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Bacillus Subtilis ◽  
Ribonucleotide Reductase ◽  
Allosteric Regulation ◽  
Cryo Electron Microscopy

scholarly journals Control of lysogeny and immunity of Bacillus subtilis temperate bacteriophage SP beta by its d gene.

1986 ◽  
Vol 167 (3) ◽  
pp. 952-959 ◽  
Author(s):  
J R McLaughlin ◽  
H C Wong ◽  
Y E Ting ◽  
J N Van Arsdell ◽  
S Chang
Keyword(s):  
Bacillus Subtilis ◽  
Temperate Bacteriophage

Ribonucleotide reductase in Bacillus subtilis - evidence for a Mn-dependent enzyme

BioFactors ◽  
1998 ◽  
Vol 7 (4) ◽  
pp. 337-344 ◽  
Author(s):  
S. F. Mohamed ◽  
O. R. Gvozdiak ◽  
D. Stallmann ◽  
U. Griepenburg ◽  
H. Follmann ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Ribonucleotide Reductase

scholarly journals Extracytoplasmic Processes Impaired by Inactivation of trxA (Thioredoxin Gene) in Bacillus subtilis

2008 ◽  
Vol 190 (13) ◽  
pp. 4660-4665 ◽  
Author(s):  
Mirja Carlsson Möller ◽  
Lars Hederstedt
Keyword(s):  
Bacillus Subtilis ◽  
Electron Donor ◽  
Ribonucleotide Reductase ◽  
Cytoplasmic Membrane ◽  
Primary Electron ◽  
Outer Side ◽  
Primary Electron Donor ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Class Ib

ABSTRACT The trxA gene is regarded as essential in Bacillus subtilis, but the roles of the TrxA protein in this gram-positive bacterium are largely unknown. Inactivation of trxA results in deoxyribonucleoside and cysteine or methionine auxotrophy. This phenotype is expected if the TrxA protein is important for the activity of the class Ib ribonucleotide reductase and adenosine-5′-phosphosulfate/3′-phosphoadenosine-5′-phosphosulfate reductase. We demonstrate here that a TrxA deficiency in addition causes defects in endospore and cytochrome c synthesis. These effects were suppressed by BdbD deficiency, indicating that TrxA in the cytoplasm is the primary electron donor to several different thiol-disulfide oxidoreductases active on the outer side of the B. subtilis cytoplasmic membrane.

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