scholarly journals Cloning the cDNA for a New Human Zinc Finger Protein Defines a Group of Closely Related Krüppel-like Transcription Factors

1998 ◽  
Vol 273 (43) ◽  
pp. 28229-28237 ◽  
Author(s):  
Nobukyuki Matsumoto ◽  
Friedrich Laub ◽  
Rafael Aldabe ◽  
Wen Zhang ◽  
Francesco Ramirez ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein

Single zinc-finger extension: enhancing transcriptional activity and specificity of three-zinc-finger proteins

2005 ◽  
Vol 386 (2) ◽  
pp. 95-99 ◽  
Author(s):  
Alexander E.F. Smith ◽  
Farzin Farzaneh ◽  
Kevin G. Ford
Keyword(s):  
Transcription Factors ◽  
Fusion Protein ◽  
Zinc Finger ◽  
Protein Interactions ◽  
Transcriptional Activation ◽  
Zinc Finger Protein ◽  
Zinc Finger Proteins ◽  
Finger Protein ◽  
Finger Extension ◽  
Vp16 Fusion

AbstractIn order to demonstrate that an existing zinc-finger protein can be simply modified to enhance DNA binding and sequence discrimination in both episomal and chromatin contexts using existing zinc-finger DNA recognition code data, and without recourse to phage display and selection strategies, we have examined the consequences of a single zinc-finger extension to a synthetic three-zinc-finger VP16 fusion protein, on transcriptional activation from model target promoters harbouring the zinc-finger binding sequences. We report a nearly 10-fold enhanced transcriptional activation by the four-zinc-finger VP16 fusion protein relative to the progenitor three-finger VP16 protein in transient assays and a greater than five-fold enhancement in stable reporter-gene expression assays. A marked decrease in transcriptional activation was evident for the four-zinc-finger derivative from mutated regulatory regions compared to the progenitor protein, as a result of recognition site-size extension. This discriminatory effect was shown to be protein concentration-dependent. These observations suggest that four-zinc-finger proteins are stable functional motifs that can be a significant improvement over the progenitor three-zinc-finger protein, both in terms of specificity and the ability to target transcriptional function to promoters, and that single zinc-finger extension can therefore have a significant impact on DNA zinc-finger protein interactions. This is a simple route for modifying or enhancing the binding properties of existing synthetic zinc-finger-based transcription factors and may be particularly suited for the modification of endogenous zinc-finger transcription factors for promoter biasing applications.

scholarly journals 1000. Down-Regulating the Epithelial Glycoprotein-2 Promoter Utilizing Engineered Zinc Finger Protein Transcription Factors

2006 ◽  
Vol 13 ◽  
pp. S385
Author(s):  
Willemijn M. Gommans ◽  
Pamela M.J. McLaughlin ◽  
Robbert Cool ◽  
Beatrice I. Lindhout ◽  
Bert van der Zaal ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein ◽  
Protein Transcription

scholarly journals Regulation of endogenous gene expression using small molecule-controlled engineered zinc-finger protein transcription factors

Gene Therapy ◽  
2007 ◽  
Vol 14 (18) ◽  
pp. 1362-1369 ◽  
Author(s):  
C L Dent ◽  
G Lau ◽  
E A Drake ◽  
A Yoon ◽  
C C Case ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Zinc Finger ◽  
Small Molecule ◽  
Zinc Finger Protein ◽  
Endogenous Gene ◽  
Finger Protein ◽  
Protein Transcription

Characterization of the isoforms of MOVO zinc finger protein, a mouse homologue of Drosophila Ovo, as transcription factors

Gene ◽  
2004 ◽  
Vol 336 (1) ◽  
pp. 47-58 ◽  
Author(s):  
Sawako Unezaki ◽  
Mikio Nishizawa ◽  
Emiko Okuda-Ashitaka ◽  
Yasuo Masu ◽  
Masanori Mukai ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Protein A ◽  
Mouse Homologue ◽  
Finger Protein

Engineering zinc finger protein transcription factors to downregulate the epithelial glycoprotein-2 promoter as a novel anti-cancer treatment

2007 ◽  
Vol 46 (5) ◽  
pp. 391-401 ◽  
Author(s):  
Willemijn M. Gommans ◽  
Pamela M.J. McLaughlin ◽  
Beatrice I. Lindhout ◽  
David J. Segal ◽  
D.J. Wiegman ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Cancer Treatment ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein ◽  
Anti Cancer ◽  
Protein Transcription

Gene regulation in planta by plant-derived engineered zinc finger protein transcription factors

2005 ◽  
Vol 57 (3) ◽  
pp. 411-423 ◽  
Author(s):  
Rachel Holmes-Davis ◽  
Guofu Li ◽  
Andrew C. Jamieson ◽  
Edward J. Rebar ◽  
Qiang Liu ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Transcription Factors ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
In Planta ◽  
Finger Protein ◽  
Protein Transcription
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