scholarly journals Structure and function of theLeptospira interrogansperoxide stress regulator (PerR), an atypical PerR devoid of a structural metal-binding site

2017 ◽  
Vol 293 (2) ◽  
pp. 497-509 ◽  
Author(s):  
Mounira Kebouchi ◽  
Frederick Saul ◽  
Raléb Taher ◽  
Annie Landier ◽  
Bénédicte Beaudeau ◽  
...  
Keyword(s):  
Binding Site ◽  
Metal Binding ◽  
Structure And Function ◽  
Metal Binding Site ◽  
Structural Metal ◽  
And Function

scholarly journals Acquisition of ionic copper by the bacterial outer membrane protein OprC through a novel binding site

PLoS Biology ◽  
2021 ◽  
Vol 19 (11) ◽  
pp. e3001446
Author(s):  
Satya Prathyusha Bhamidimarri ◽  
Tessa R. Young ◽  
Muralidharan Shanmugam ◽  
Sandra Soderholm ◽  
Arnaud Baslé ◽  
...  
Keyword(s):  
Binding Site ◽  
Outer Membrane ◽  
Metal Binding ◽  
Infection Model ◽  
Metal Binding Site ◽  
Ionic Copper ◽  
Wide Range ◽  
Bacterial Outer Membrane ◽  
And Function ◽  
Copper Import

Copper, while toxic in excess, is an essential micronutrient in all kingdoms of life due to its essential role in the structure and function of many proteins. Proteins mediating ionic copper import have been characterised in detail for eukaryotes, but much less so for prokaryotes. In particular, it is still unclear whether and how gram-negative bacteria acquire ionic copper. Here, we show that Pseudomonas aeruginosa OprC is an outer membrane, TonB-dependent transporter that is conserved in many Proteobacteria and which mediates acquisition of both reduced and oxidised ionic copper via an unprecedented CxxxM-HxM metal binding site. Crystal structures of wild-type and mutant OprC variants with silver and copper suggest that acquisition of Cu(I) occurs via a surface-exposed “methionine track” leading towards the principal metal binding site. Together with whole-cell copper quantitation and quantitative proteomics in a murine lung infection model, our data identify OprC as an abundant component of bacterial copper biology that may enable copper acquisition under a wide range of conditions.

scholarly journals Altered Active Site Flexibility and a Structural Metal-binding Site in Eukaryotic dUTPase

2004 ◽  
Vol 279 (17) ◽  
pp. 17932-17944 ◽  
Author(s):  
Júlia Kovári ◽  
Orsolya Barabás ◽  
Enikõ Takács ◽  
Angéla Békési ◽  
Zsófia Dubrovay ◽  
...  
Keyword(s):  
Binding Site ◽  
Active Site ◽  
Metal Binding ◽  
Metal Binding Site ◽  
Structural Metal

Unraveling the Substrate−Metal Binding Site of Ferrochelatase:  An X-ray Absorption Spectroscopic Study†

Biochemistry ◽  
2002 ◽  
Vol 41 (15) ◽  
pp. 4809-4818 ◽  
Author(s):  
Gloria C. Ferreira ◽  
Ricardo Franco ◽  
Arianna Mangravita ◽  
Graham N. George
Keyword(s):  
Spectroscopic Study ◽  
Binding Site ◽  
Metal Binding ◽  
Metal Binding Site ◽  
X Ray ◽  
Substrate Metal ◽  
X Ray Absorption

Construction of an additional metal-binding site in human metallothionein-2

2006 ◽  
Vol 101 (4) ◽  
pp. 354-360 ◽  
Author(s):  
Mitsutoshi Toyama ◽  
Mariko Sasaki ◽  
Noriaki Hirayama ◽  
Yoshikatsu Murooka ◽  
Mitsuo Yamashita
Keyword(s):  
Binding Site ◽  
Metal Binding ◽  
Metal Binding Site
Sign in / Sign up

Export Citation Format

Share Document