Development of a Self-assembling Nuclear Targeting Vector System Based on the Tetracycline Repressor Protein

Here, we present a simple modular extendable vector system for introducing the T7 RNA polymerase and tetracycline repressor genes into Trypanosoma brucei . This novel system exploits developments in our understanding of gene expression and genome organization to produce a streamlined plasmid optimized for high levels of expression of the introduced transgenes. We demonstrate the utility of this novel system in bloodstream and procyclic forms of Trypanosoma brucei , including the genome strain TREU927/4. We validate these cell lines using a variety of inducible experiments that recapture previously published lethal and non-lethal phenotypes. We further demonstrate the utility of the single marker (SmOx) TREU927/4 cell line for in vivo experiments in the tsetse fly and provide a set of plasmids that enable both whole-fly and salivary gland-specific inducible expression of transgenes.

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Biophysical and biochemical characterization of Rv3405c, a tetracycline repressor protein from Mycobacterium tuberculosis

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2018.01.152 ◽

2018 ◽

Vol 496 (3) ◽

pp. 799-805 ◽

Cited By ~ 1

Author(s):

Akilandeswari Gopalan ◽

Raghu Bhagavat ◽

Nagasuma Chandra ◽

Savithri Handanahal Subbarao ◽

Alamelu Raja ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Biochemical Characterization ◽

Repressor Protein ◽

Tetracycline Repressor

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Intrinsically Fluorescent Carbon Nanospheres as a Nuclear Targeting Vector: Delivery of Membrane-Impermeable Molecule to Modulate Gene Expression In Vivo

Nano Letters ◽

10.1021/nl801503m ◽

2008 ◽

Vol 8 (10) ◽

pp. 3182-3188 ◽

Cited By ~ 155

Author(s):

B. Ruthrotha Selvi ◽

Dinesh Jagadeesan ◽

B. S. Suma ◽

G. Nagashankar ◽

M. Arif ◽

...

Keyword(s):

Gene Expression ◽

Nuclear Targeting ◽

Carbon Nanospheres ◽

Targeting Vector ◽

Vector Delivery ◽

Fluorescent Carbon

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Torus Circumference and Thickness Parameters From a Linear DNA Size Series Suggest How Toruses Self-Assemble

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100119430 ◽

1985 ◽

Vol 43 ◽

pp. 522-523

Author(s):

George C. Ruben ◽

Kenneth A. Marx

Keyword(s):

Tertiary Structure ◽

Dna Complexes ◽

Tertiary Structures ◽

Self Assembling ◽

Double Stranded Dna ◽

Calf Thymus ◽

Dna Organization ◽

Condensed Dna ◽

Dna Size

Certain double stranded DNA bacteriophage and viruses are thought to have their DNA organized into large torus shaped structures. Morphologically, these poorly understood biological DNA tertiary structures resemble spermidine-condensed DNA complexes formed in vitro in the total absence of other macromolecules normally synthesized by the pathogens for the purpose of their own DNA packaging. Therefore, we have studied the tertiary structure of these self-assembling torus shaped spermidine- DNA complexes in a series of reports. Using freeze-etch, low Pt-C metal (10-15Å) replicas, we have visualized the microscopic DNA organization of both calf Thymus( CT) and linear 0X-174 RFII DNA toruses. In these structures DNA is circumferentially wound, continuously, around the torus into a semi-crystalline, hexagonal packed array of parallel DNA helix sections.

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Structure–property studies of self-assembling [n.n]paracyclophanes

10.1021/scimeetings.0c06518 ◽

2020 ◽

Author(s):

Will R Henderson ◽

Danielle E. Fagnani ◽

Yu Zhu ◽

Guancen Liu ◽

Ronald K. Castellano

Keyword(s):

Structure Property ◽

Self Assembling

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Self assembling mirrors

Nature ◽

10.1038/news990729-4 ◽

1999 ◽

Author(s):

Philip Ball

Keyword(s):

Self Assembling

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Designing Enzyme-Triggered Hydrogels for Biomedical Applications Using Self-Assembling Octapeptides

10.1557/proc-1140-hh09-01 ◽

2008 ◽

Author(s):

Aline Fiona. Miller ◽

Elisabeth Vey ◽

Alberto Saiani

Keyword(s):

Biomedical Applications ◽

Self Assembling

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Combinatorial Peptide Libraries for Selecting Inorganic-Binding Proteins: A Step in Molecular Biomimetics

MRS Proceedings ◽

10.1557/proc-773-n8.1 ◽

2003 ◽

Vol 773 ◽

Cited By ~ 1

Author(s):

C. Tamerler ◽

S. Dinçer ◽

D. Heidel ◽

N. Karagûler ◽

M. Sarikaya

Keyword(s):

Binding Proteins ◽

Building Blocks ◽

Molecular Engineering ◽

Inorganic Materials ◽

Combinatorial Peptide Libraries ◽

Self Assembling ◽

Complex Functions ◽

Recent Developments ◽

Specific Proteins ◽

Molecular Biomimetics

AbstractProteins, one of the building blocks in organisms, not only control the assembly in biological systems but also provide most of their complex functions. It may be possible to assemble materials for practical technological applications utilizing the unique advantages provided by proteins. Here we discuss molecular biomimetic pathways in the quest for imitating biology at the molecular scale via protein engineering. We use combinatorial biology protocols to select short polypeptides that have affinity to inorganic materials and use them in assembling novel hybrid materials. We give an overview of some of the recent developments of molecular engineering towards this goal. Inorganic surface specific proteins were identified by using cell surface and phage display technologies. Examples of metal and metal oxide specific polypeptides were represented with an emphasis on certain level of specificities. The recognition and self assembling characteristics of these inorganic-binding proteins would be employed in develeopment of hybrid multifunctional materials for novel bio- and nano-technological applications.

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