Aluminium distribution in soybean root tip for a short time Al treatment

2001 ◽  
Vol 158 (6) ◽  
pp. 731-736 ◽  
Author(s):  
Tatsuhiko Kataoka ◽  
Tomoko M. Nakanishi
Keyword(s):  
Root Tip ◽  
Soybean Root ◽  
Short Time ◽  
Al Treatment

INTERACTION BETWEEN SILICON AND ALUMINUM IN TRITICUM AESTIVUM L. (CV. CELTIC)

1997 ◽  
Vol 45 (4) ◽  
pp. 285-292 ◽  
Author(s):  
Kay M. Cocker ◽  
Martin J. Hodson ◽  
David E. Evans ◽  
Allan G. Sangster
Keyword(s):  
Triticum Aestivum ◽  
Continuous Flow ◽  
Cell Walls ◽  
Dry Weight ◽  
Triticum Aestivum L ◽  
Test Solution ◽  
Root Tip ◽  
Solution Ph ◽  
Al Content ◽  
Al Treatment

Seedlings ofTriticum aestivumL. (cv. Celtic) were suspended in plastic tubs containing 500 μmol L−1Ca(NO3)2and 31 μmol L−1KC1 as background solution. A1C13(0 and 100 μmol L−1) and Na2SiO3.5H2O (0 and 2000 μmol; L−1) were added to this basal nutrient medium, and solution pH was set at 4.2 or 4.6. Tubs were aerated and supplied with a continuous flow of pH-adjusted test solution. Plants were grown for 4 d in a growth cabinet at 25 °C with a 16 h photoperiod. At pH 4.2 and 4.6 root length of the seedlings was inhibited at 100 μmol L−1Al. An amelioration of Al-induced toxicity symptoms was observed in the 100 μmol L−1A1/2000 μmol L−1Si treatment at pH 4.6, but not at pH 4.2. Both the shoot (S) and root (R) dry weight of seedlings treated with 100 μmol L−1Al were reduced when compared with controls. Treatment with Al increased S:R ratios, and this effect was ameliorated by Si, but only at pH 4.6.Al content of roots treated with 100 μmol L−1Al or 100 μmol L−1Al/2000 μmol L−1Si increased significantly when compared with controls. More Al accumulated in the roots of seedlings of the 100 μmol L−1Al/2000 μmol L−1Si treatment than in the 100 μmol L−1treatment. Al treatment reduced root and shoot K concentrations under both pH regimes, and Si did not ameliorate this effect. Al treatment had little effect on seedling Ca levels.Three treatments were selected for a microanalytical investigation of the basal third of the root, and the zone 3.5 mm behind the root tip: 2800 μmol L−1Si; 75 μmol Al; and a combination of the two. When plants were grown in 2800 μmol L−1Si the major silica deposition sites in the roots were the endodermal walls. In the 75 μmol L−1Al treatment, Al was mainly located in the epidermal and hypodermal walls. Al treatment caused a leakage of phosphorus into these cell walls. When both 2800 μmol L−1and 75 μmol L−1Al were present in the nutrient solution, only Si was deposited in the endodermal walls, while both elements were present in the epidermal walls. Leakage of phosphorus appeared to be prevented in the presence of Si.

Aluminium-inhibited NO3– uptake is related to Al-increased H2O2 content and Al-decreased plasma membrane ATPase activity in the root tips of Al-sensitive black soybean

2017 ◽  
Vol 44 (2) ◽  
pp. 198 ◽  
Author(s):  
Dan Yang ◽  
Dongjie Chen ◽  
Ping Wang ◽  
Daihua Jiang ◽  
Huini Xu ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Atpase Activity ◽  
Treatment Time ◽  
Root Tips ◽  
Black Soybean ◽  
Soybean Root ◽  
H2o2 Content ◽  
Al Stress ◽  
Pump Activity ◽  
Al Treatment

In this study, Al-sensitive black soybean (Glycine max (L.) Merr.) specimens were treated in Hoagland solutions containing 50–400 µM Al for 1–4 days. The measurement for NO3– uptake showed that the NO3– uptake decreased gradually as the Al concentration and treatment time increased, suggesting that Al stress significantly reduced the NO3– uptake by soybean. Under 100-µM Al stress for 4 days, the plasma membrane (PM) ATPase activity (inorganic phosphate (Pi) release), H+ pump activity, phosphorylation of PM ATPase and its interaction with 14-3-3 protein in soybean root tips were all smaller than those in the root tips of control plants. The addition of 150 µM Mg2+ in Al treatment solutions significantly alleviated the Al inhibition of NO3– uptake in soybean. The presence of Mg2+ in a 100-µM Al solution pronouncedly enhanced PM ATPase activity, H+ pump activity, phosphorylation of PM ATPase and its interaction with 14-3-3 protein in soybean root tips. The application of 2 mM ascorbic acid (AsA, an H2O2 scavenger) in Al treatment solutions significantly decreased Al-inhibited NO3– uptake in soybean. The cotreatment of soybeans with 2 mM AsA and 100 µM Al significantly reduced H2O2 accumulation and increased the PM ATPase activity, H+ pump activity, phosphorylation of PM H+-ATPase and its interaction with 14-3-3 protein in soybean root tips. The evidence suggested that Al-inhibited NO3– uptake is related to Al-increased H2O2 content and Al-decreased phosphorylation of PM ATPase and its interaction with 14-3-3 protein as well as PM ATPase activity in the root tips of soybean.

Transcriptome profiling of soybean root tips

2011 ◽  
Vol 38 (6) ◽  
pp. 451 ◽  
Author(s):  
Farzad Haerizadeh ◽  
Mohan B. Singh ◽  
Prem L. Bhalla
Keyword(s):  
Gene Expression ◽  
Expression Profile ◽  
Target Genes ◽  
Animal Feed ◽  
Positive Impact ◽  
Root Apical Meristem ◽  
Root Tip ◽  
Symbiotic Association ◽  
Root Tips ◽  
Soybean Root

Soybean (Glycine max L.), a major legume crop, is important to human nutrition and is a source of animal feed. Similar to many legumes, a key feature of the soybean is its symbiotic association with soil bacteria that fix atmospheric nitrogen. However, knowledge of the gene expression of its root system, particularly the root meristematic region, is limited. Here, we have addressed this by investigating the gene expression profile of the soybean root tip, using soybean Affymetrix chips containing 37 500 probe sets (Affymetrix Inc.) and have compared this expression profile with that of the nonmeristematic tissue. We identified a total of 5012 upregulated and 4136 downregulated genes in the soybean root tip. Among the upregulated genes, 559 showed strong preferential expression in the root tip, indicating that they are likely to be associated with root apical meristem specificity and root tip function. Genes involved in membrane transport, defence signalling and metabolism were upregulated in the soybean root tip. Further, our data provide a resource of novel target genes for further studies involving root development and biology, and will possibly have a positive impact on future crop breeding.

Vacuole Biogenesis in Living Soybean Root Tip Cells

2005 ◽  
Vol 11 (S02) ◽  
Author(s):  
R H Berg ◽  
N Walter ◽  
C G Taylor
Keyword(s):  
Root Tip ◽  
Soybean Root ◽  
Vacuole Biogenesis ◽  
Tip Cells ◽  
Root Tip Cells

The Decrease of Extracted Apoplast Protein in Soybean Root Tip by Aluminium Treatment

2003 ◽  
Vol 46 (3) ◽  
pp. 445-449 ◽  
Author(s):  
T. Kataoka ◽  
J. Furukawa ◽  
T.M. Nakanishi
Keyword(s):  
Root Tip ◽  
Soybean Root

What the replication reformation wrought

2018 ◽  
Vol 41 ◽  
Author(s):  
Barbara A. Spellman ◽  
Daniel Kahneman
Keyword(s):  
Reform Movement ◽  
Short Time

AbstractReplication failures were among the triggers of a reform movement which, in a very short time, has been enormously useful in raising standards and improving methods. As a result, the massive multilab multi-experiment replication projects have served their purpose and will die out. We describe other types of replications – both friendly and adversarial – that should continue to be beneficial.

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