The in vitro metabolism of 25-hydroxycholecalciferol by pig kidney: effect of low dietary levels of calcium and phosphorus

1. Homogenates of pig kidney incubated in vitro convert 25-hydroxycholecalciferol to the dihydroxy-metabolites almost as efficiently as homogenates of chick kidney under similar conditions.2. The cortex of the kidney carries out this conversion more efficiently than does the medulla.3. Kidneys from pigs given low calcium or low phosphorus diets produce about 40 % more 1,25-dihydroxy cholecalciferol than those from pigs given diets containing normal levels of Ca and P.

Download Full-text

In Vitro Stimulation of 25-Hydroxycholecalciferol lα- Hydroxylation by Parathyroid Hormone in Chick Kidney Slices: Evidence for a Role for Adenosine 3′,5′- Monophosphate *

Endocrinology ◽

10.1210/endo-108-3-1002 ◽

1981 ◽

Vol 108 (3) ◽

pp. 1002-1006 ◽

Cited By ~ 62

Author(s):

CHARLES R. ROST ◽

DANIEL D. BIKLE ◽

ROY A. KAPLAN

Keyword(s):

Parathyroid Hormone ◽

Kidney Slices ◽

Chick Kidney ◽

25 Hydroxycholecalciferol ◽

Stimulation Of

Download Full-text

Uric acid, calcium and phosphorus clearances in normal subjects on a low calcium, low phosphorus diet: Uric acid, calcium and phosphorus clearances after calcium infusion in normal and gouty patients

Metabolism ◽

10.1016/0026-0495(65)90060-0 ◽

1965 ◽

Vol 14 (3) ◽

pp. 203-210 ◽

Cited By ~ 4

Author(s):

Cristobal G. Duarte ◽

John H. Bland

Keyword(s):

Uric Acid ◽

Normal Subjects ◽

Low Calcium ◽

Low Phosphorus ◽

Calcium Infusion ◽

Calcium And Phosphorus

Download Full-text

Effects of normal and low calcium and phosphorus levels and 25-hydroxycholecalciferol supplementation on performance, serum antioxidant status, meat quality, and bone properties of broilers

Poultry Science ◽

10.1016/j.psj.2020.07.008 ◽

2020 ◽

Vol 99 (11) ◽

pp. 5663-5672

Author(s):

L.H. Zhang ◽

T.F. He ◽

J.X. Hu ◽

M. Li ◽

X.S. Piao

Keyword(s):

Meat Quality ◽

Antioxidant Status ◽

Low Calcium ◽

Bone Properties ◽

Calcium And Phosphorus ◽

25 Hydroxycholecalciferol ◽

Phosphorus Levels

Download Full-text

Metabolism in Vitro of 25-Hydroxycholecalciferol in Chicks Fed on Phosphorus-Deficient Diets

Clinical Science ◽

10.1042/cs0540197 ◽

1978 ◽

Vol 54 (2) ◽

pp. 197-200 ◽

Cited By ~ 2

Author(s):

R. Swaminathan ◽

Barbara A. Sommerville ◽

A. D. Care

Keyword(s):

Calcium Absorption ◽

Plasma Calcium ◽

Hydroxylase Activity ◽

High Phosphorus ◽

Phosphorus Deprivation ◽

Low Phosphorus ◽

Loop Technique ◽

25 Hydroxycholecalciferol

1. Three groups of 10-days-old chicks were fed on one of three diets having phosphorus contents of 0·08 mol/kg, 0·14 mol/kg or 0·21 mol/kg. Ten days later duodenal calcium absorption by the ligated loop technique in vivo, and plasma calcium and phosphorus concentrations, were measured. In addition the metabolism in vitro of 25-hydroxycholecalciferol [25-(OH)D3] by kidney homogenates was studied. 2. In the low phosphorus group (0·08 mol/kg) calcium absorption and the activity of 25-(OH)D3-1-hydroxylase were significantly higher than those of the high phosphorus group (0·21 mol/kg). However, in the medium phosphorus group (0·14 mol/kg), calcium absorption was significantly higher although the activity of 25-(OH)D3-1-hydroxylase was not significantly higher when compared with the high phosphorus group (0·21 mol/kg). 3. It is concluded that in phosphorus deprivation, unlike in calcium deprivation, a diet very low in phosphorus is required to stimulate the renal 25-(OH)D3-1-hydroxylase activity.

Download Full-text