A Rapid Method for Determination of Progesterone By Reversed-Phase Liquid Chromatography from Aqueous Media

2000 ◽  
Vol 33 (5) ◽  
pp. 881-889 ◽  
Author(s):  
G. R. Pereira ◽  
J. M. Marchetti ◽  
M. V. L. B. Bentley*
Keyword(s):  
Liquid Chromatography ◽  
Rapid Method ◽  
Aqueous Media ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid

Liquid Chromatographic Assay of Dimetridazole, Ipronidazole, and Ronidazole in Feeds and Premixes

1992 ◽  
Vol 75 (4) ◽  
pp. 663-666 ◽  
Author(s):  
B Tollomelli ◽  
R Laffi ◽  
S Rossato ◽  
G Colpo
Keyword(s):  
Liquid Chromatography ◽  
Standard Deviation ◽  
Rapid Method ◽  
Reversed Phase ◽  
Uv Detection ◽  
Reversed Phase Liquid Chromatography ◽  
Alumina Column ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A rapid method has been developed for the determination of dimetridazole (DMZ), ipronidazole (IPZ), and ronidazole (RNZ) in turkey feeds, swine feeds, and premix. The compounds are extracted from samples with warm methanol, the extract is purified over a short alumina column, and an aliquot of the eluate is analyzed by reversed-phase liquid chromatography and UV detection at 309 nm. Alumina cleanup of premixes is not essential, although the resulting chromatograms are cleaner. Recoveries of DMZ from feed formulations ranged from 97 to 103% at the 10.0,50.0, and 100.0 ppm levels, with a standard deviation (SD) of 0.62-3.2%. Recoveries of IPZ ranged from 94.4 to 101.2% at the 5.0,50.0, and 100.0 ppm levels (SD, 0.42-4.4%). RNZ recoveries ranged from 95 to 100.7% at the 6.0,60.0, and 120.0 ppm levels (SD, 1.2-5.33%).

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