In this collaborative study we have investigated the coexistence of two different markers of fibrinogen to fibrin conversion in 19 patients with liver cirrhosis, confirmed with liver biopsy, and classified according to standardized criteria into 11 moderate and 8 severe cases. Sepharose gel filtration of plasma allowed separation of fibrinogen-like material (FLM) into high m.w. (HMWF), peak, and low m.w. (LMWF) fractions, quantitated as percent (w/w) of the total FLM eluted. FPA was assayed radioimmunologically.HMWF was markedly increased in the whole group of cirrhotics (av ± SD 10.4% ± 2.77) , and in both moderate and severe patients, vs controls (6.9% ± 1.39; n=16; p < 0.01 for each comparison). LMWF was moderately increased in patients (6.8% ± l.84) vs controls (5.3% ± 1.35; p < 0.05).FPA levels (controls: median (m) ng/ml 2.2; m confidence limits (mcl) 1.4-2.5; range (r) < 0.6 to 4.1) showed an increase in cirrhotics (m 2.8; mcl 2.64-3.35, r<0.6 to 94) but the difference was not statistically significant using a non-parametric test. However, in 6 out of 19 patients FPA values exceeded av ± 2 SD of controls (av ± SD 2.05 ± 1.15). No significant correlations were found between FPA and HMWF or LMWF levels.The lack of correlation between FPA and HMWF although possibly related to half-life differences, suggests that, besides thrombin effect, other mechanisms for excess formation and/or accumulation of HMWF, as dysproteinaemia, dys- fibrinogenaemia, or impaired clearance, should be considered.