Coconut milk enhancement of axillary shoot growth in vitro of kiwifruit

Shoot proliferation cultures were established in vitro using flower-stem explants from two different interspecific hybrid plants of Liatris. Explants taken on two dates from field-grown plants were successfully established and axillary shoot growth promoted on a medium consisting of Murashige and Skoog basal salts and vitamins with 30 g·L-1 sucrose, 1.0 μm BA, and 7.0 g·L-1 agar, with a medium pH = 5.7. Initial explant contamination rates were significantly higher among explants collected later in the growing season. Addition of BA (1.0, 2.0, 4.0, 8.0, or 16.0 μm) improved shoot formation compared to the control for both plants. Proliferation rates differed between the dates of establishment, the plants, and the BA treatments. Shoots rooted readily in medium without PGRs or with 1.0, 2.0, 4.0, or 8.0 μm K-IBA. Overall rooting was 88%. About 90% of the plants rooted in the presence of 1.0 μm K-IBA were successfully established in the greenhouse. Chemical names used: 6-benzyl adenine (BA); potassium salt of indole-3-butyric acid (K-IBA).

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HORMONAL CONTROL OF AXILLARY BUD DORMANCY IN STRAWBERRY - AXILLARY SHOOT GROWTH AND DEVELOPMENT IN IN VITRO CULTURES OF TRANSGENIC STRAWBERRY CARRYING MAIZE IAA-GLUCOSE SYNTHETASE GENE

Acta Horticulturae ◽

10.17660/actahortic.2004.649.30 ◽

2004 ◽

pp. 165-168

Author(s):

L. Michalczuk ◽

D. Wawrzyńczak

Keyword(s):

Growth And Development ◽

Shoot Growth ◽

Hormonal Control ◽

In Vitro Cultures ◽

Bud Dormancy ◽

Axillary Bud ◽

Axillary Shoot

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Callus Induction and Plant Regeneration in Rauvolfia Serpentina (L.) Benth Ex. Kurz.

Journal of Natural History Museum ◽

10.3126/jnhm.v24i1.2245 ◽

2009 ◽

Vol 24 ◽

pp. 82-88 ◽

Cited By ~ 1

Author(s):

Saraswoti Aryal ◽

Sanu Devi Joshi

Keyword(s):

Acetic Acid ◽

Callus Induction ◽

Coconut Milk ◽

Ms Medium ◽

Rauvolfia Serpentina ◽

History Museum ◽

Short Period ◽

Murashige Skoog ◽

Callus Tissue Culture

Rauvolfia serpentina (L.) ex. Kurz is an important medicinal plant. Callus induction and regeneration was studied from stem explant of in-vitro grown plant of Rauvolfia serpentina(L.) Benth. ex Kurz (Apocynaceae) on Murashige Skoog (1962) medium supplemented with 1mg/l 2,4-Dichlorophenocy acetic acid (2,4-D) and 1mg/l Kinetin (Kn). Vigorous growth of callus occurs after 4 weeks of culture. Callus was sub-cultured on Murashige and Skoog (MS) medium supplemented with different concentration of 2, 4-D (0.5-3.0 mg/l) and 10% coconut milk. Regeneration of plantlets occurred on MS medium containing 3 mg/1 of 2, 4-D and 10% coconut milk. These plantlets were rooted on MS medium supplemented with 1 mg/l IAA .The regenerated plantlets were able to grow on soil after short period ofacclimatization. Key words: Explant; In-vitro culture; MS medium; 2, 4 Dichlorophenoxy acetic acid; Kinetin; Callus; Tissue culture; Coconut milk. Journal of Natural History Museum Vol. 24, 2009 Page: 82-88

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Selectivity of auxin for induction and growth of callus from excised embryo of spring and winter wheat

Canadian Journal of Botany ◽

10.1139/b84-189 ◽

1984 ◽

Vol 62 (7) ◽

pp. 1393-1397 ◽

Cited By ~ 6

Author(s):

M. D. Zhou ◽

T. T. Lee

Keyword(s):

Winter Wheat ◽

Chinese Spring ◽

Shoot Growth ◽

Callus Formation ◽

Triticum Aestivum L ◽

Dichlorophenoxyacetic Acid ◽

Naphthaleneacetic Acid ◽

2,4 Dichlorophenoxyacetic Acid ◽

Methoxybenzoic Acid

The callus-promoting activity of most commonly known as well as some rarely tested auxins was compared with that of 2,4-dichlorophenoxyacetic acid (2,4-D) for in vitro culture of the excised embryo of spring and winter wheat (Triticum aestivum L.), cv. Chinese Spring and cv. Fredrick. Different auxins in a concentration range from 1 to 50 μM showed widely different activities. Also the two wheat cultivars responded differently to the auxins. When rapid callus formation with limited root growth was used as the basis for comparison, 2-(2-methyl-4-chlorophenoxy)propionic acid (2-MCPP), α-naphthaleneacetic acid, 3,6-dichloro-2-methoxybenzoic acid (dicamba), 4-amino-3,5,6,trichloropicolinic acid (picloram), γ-(2,4-dichlorophenoxy)butyric acid, 2,4,5-trichlorophenoxyacetic acid, and 2,4,5-trichlorophenoxypropionic acid, in the order of effectiveness, were superior to 2,4,-D for callus induction from the embryo of 'Chinese Spring,' although the concentration required was higher than that of 2,4-D. For the winter wheat 'Fredrick,' however, only picloram, dicamba, and 2-MCPP performed as well as 2,4-D. All auxins tested promoted shoot growth; 2-methyl-4-chlorophenoxypropionic acid was most effective for 'Chinese Spring,' whereas picloram was most effective for 'Fredrick.'

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Influence of preculture treatment and types of explants on shoot growth and in vitro flowering of feathered amaranth (Celosia argentea var. plumose)

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-010-9883-5 ◽

2010 ◽

Vol 105 (3) ◽

pp. 465-469 ◽

Cited By ~ 6

Author(s):

Kitti Bodhipadma ◽

Sompoch Noichinda ◽

Winan Padyencheun ◽

Theerapong Khunthacharoen ◽

Utorn Chikhunthod ◽

...

Keyword(s):

Shoot Growth ◽

In Vitro Flowering ◽

Celosia Argentea

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Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d211248 ◽

2020 ◽

Vol 21 (12) ◽

Author(s):

Erma Prihastanti ◽

ENDAH D. HASTUTI ◽

SRI W.A. SUEDY

Keyword(s):

In Vitro Culture ◽

Shoot Growth ◽

Short Communication ◽

Stem Explants ◽

Citrus Reticulata ◽

Culture Methods ◽

Treatment Groups ◽

Physiological Conditions ◽

Growth Differences

Abstract. Prihastanti E, Hastuti ED, Suedy SWA. 2020. Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods. Biodiversitas 21: 5845-5849. Several efforts have been made to preserve Citrus reticulata var. Tawangmangu and Citrus reticulata var. Garut as indigenous Indonesian mandarin cultivars, including in vitro tissue culture methods. This study aimed to determine growth differences of the stem explants of C. reticulata var. Tawangmangu and C. reticulata var. Garut,which planted on the same Murashige and Skoog (MS) media. The treatment groups were derived from different explants, grown in 4 separate culture bottles for 35 days at 25°C. The observed parameters included the percentage of explants indicating callus development browned-colored explants, and the contaminated explants. Among C. reticulata var. Tawangmangu explants, 23.53% indicated callus development, 29.42% were browned-colored explants, and 0% indicated contamination. In contrast, among the C. reticulata var. Garut explants, 0% indicated callus development, 7.14% brown-colored, and 7.14% indicated contamination. The stems explants from C. reticulata var. Tawangmangu showed a tendency to develop calluses, but the explants of C. reticulata var. Garut was able to support the growth of shoots. C. reticulata var. Tawangmangu and Garut mandarin stem explants showed differences of shoot growth because physiological conditions varied according to the variety.

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Diazotrophic bacteria in the growth of micropropagated ornamental pineapple

Revista Colombiana de Ciencias Hortícolas ◽

10.17584/rcch.2019v13i2.8034 ◽

2019 ◽

Vol 13 (2) ◽

pp. 269-278

Author(s):

Adriano Bortolotti Silva ◽

Ligiane Aparecida Florentino ◽

Dalvana De Sousa Pereira ◽

Paulo Roberto Correa Landgraf ◽

Ana Carolina Rodrigues Alves ◽

...

Keyword(s):

Shoot Growth ◽

Ex Vivo ◽

Control Treatment ◽

In Vitro Cultivation ◽

Ms Medium ◽

Ex Vitro ◽

Diazotrophic Bacteria ◽

Bacterial Strains ◽

Indole 3 Acetic Acid

Ornamental pineapple is a hardy plant with significant landscaping value. Tissue culture of plants is viable for producing plants with a high phytosanitary quality. However, one of the difficulties with this cultivar is the acclimatization process, which is slow and can cause losses. The objective of the present study was to verify the potential of inoculation with diazotrophic bacteria for in vitro and ex vivo growth of ornamental pineapple. A group of diazotrophic bacterial strains selected at the Universidade José do Rosário Vellano (UNIFENAS) was prioritized in this study, and the treatments included bacterial strains UNIFENAS (100-13, 100-60, 100-68, 100-153, 100-167 and 100-198). These strains were evaluated in terms of their capacity to produce indole 3-acetic acid. Subsequently, plants were cultivated in a medium composed of MS medium salts (1/4), adding 1 mL of the bacterial strain. In the control treatment, the plants were maintained in 2 mL of MS medium. 7 days after inoculation, the plants were transplanted into the MS, where they were maintained for 30 days. After in vitro cultivation, the plants were transferred to pots containing commercial Plantmax® substrate and maintained under these conditions for 60 days. The diazotrophic bacteria were able to synthesize auxins, and their inoculation promoted greater growth in vitro and ex vitro in the plants. In the acclimatization phase, the plants inoculated with UNIFENAS strains (100-60, 100-68 and 100-153) promoted a higher shoot growth, chlorophyll content and nitrate reductase enzyme activity.

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In vitro Callus Induction and Regeneration of Popular Indica Rice Genotypes

International Journal of Agriculture Environment and Biotechnology ◽

10.30954/0974-1712.04.2020.2 ◽

2020 ◽

Vol 13 (4) ◽

Author(s):

Aananthi. N

Keyword(s):

Callus Induction ◽

Callus Formation ◽

Indica Rice ◽

Immature Embryo ◽

Coconut Milk ◽

Rice Genotypes ◽

Mist Chamber ◽

Regenerated Plant ◽

Better Than

Five rice cultivars viz., ASD 16, White Ponni, Pusa Basmati 1, Pusa Sugandh 4 and Pusa Sugandh 5 belonging to subspecies indica were compared for its ability in callus formation and regeneration. In this experiment, the different parameters viz., the effect of hormones (2,4-D and kinetin), organic supplement (coconut milk O1-CM 100 mll-1, O2-CM 75 mll-1, O3-CM 50 mll-1), explants (seed and immature embryo), media (MS and N6), carbon source (sucrose and maltose) using five genotypes on callus response was studied. The effect of hardening methods was also assessed. Results showed that for enhanced callus induction was with MS medium supplemented with 2.0 mgl-1 2, 4-D + 0.5 mgl-1 kinetin + 30 gl-1 maltose irrespective of explants used. Addition of 100 ml l-1 coconut milk was found have improvement in callus response. The performance of immature embryo was better than seed for callus induction, emrbyogenic callus formation, rhizogenic callus formation and regeneration. MS media provided superiority over N6. Among the genotypes Pusa Basmati 1 rendered outstanding performance in callus behavior. The treatment combination MS + 2.5 mgl-1 BAP + 0.5 mgl-1 NAA + 1.0 mgl-1 KN gave the highest organogenesis response and regeneration of plantlets. Hardening in mist chamber was recognized as the best method to give the highest per cent of regenerated plant lets.

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