FTIR Spectroscopic Study of Biogenic Mn-Oxide Formation byPseudomonas putidaGB-1

Sorption of Co(II) on the biogenic Mn oxide produced by a Paraconiothyrium sp.-like strain was investigated. The biogenic Mn oxide, which was characterized to be poorly crystalline birnessite (Na4Mn(III) 6Mn(IV) 8O27 ·9H2O) bearing Mn(III) and Mn(IV) in the structure, showed approximately 6.0-fold higher efficiency for Co(II) sorption than a synthetic Mn oxide. XP-spectra of Co 2p for the biogenic and synthetic Mn oxides after Co(II) sorption indicate that Co was immobilized as Co(III) on the surface of Mn oxides, clearly suggesting that redox reaction occurs between Co(II) ions and each Mn oxides. The Co(II) ions would be initially sorbed on the vacant sites of the surface of biogenic Mn oxide, and then oxidized to Co(III) by neighbor Mn(III/IV) atoms to release Mn(II). For the synthetic Mn oxide, release of Mn(II) was negligibly small because the oxidant is only Mn(IV) in ramsdellite (γ-MnO2). The Mn(II) release from the biogenic Mn oxide during Co(II) adsorption would be not only from weakly bounded Mn(II), but also from redox reaction between Mn(III/IV) and Co(II) ions.

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Synthesis of MnO/C/NiO-Doped Porous Multiphasic Composites for Lithium-Ion Batteries by Biomineralized Mn Oxides from Engineered Pseudomonas putida Cells

Nanomaterials ◽

10.3390/nano11020361 ◽

2021 ◽

Vol 11 (2) ◽

pp. 361

Author(s):

Jin Liu ◽

Tong Gu ◽

Li Li ◽

Lin Li

Keyword(s):

Pseudomonas Putida ◽

Photoelectron Spectroscopy ◽

Coulombic Efficiency ◽

Surface Display ◽

Lithium Ion ◽

Cell Surface Display ◽

Mn Oxide ◽

Biomineralization Process ◽

Biogenic Mn Oxide ◽

Mn Oxides

A biotemplated cation-incoporating method based on bacterial cell-surface display technology and biogenic Mn oxide mineralization process was developed to fabricate Mn-based multiphasic composites as anodes for Li-ion batteries. The engineered Pseudomonas putida MB285 cells with surface-immobilized multicopper oxidase serve as nucleation centers in the Mn oxide biomineralization process, and the Mn oxides act as a settler for incorporating Ni ions to form aggregates in this process. The assays using X-ray photoelectron spectroscopy, phase compositions, and fine structures verified that the resulting material MnO/C/NiO (CMB-Ni) was porous multiphasic composites with spherical and porous nanostructures. The electrochemical properties of materials were improved in the presence of NiO. The reversible discharge capacity of CMB-Ni remained at 352.92 mAh g−1 after 200 cycles at 0.1 A g−1 current density. In particular, the coulombic efficiency was approximately 100% after the second cycle for CMB-Ni.

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Synthesis of Biogenic Mn Oxide and its Application as Lithium Ion Sieve

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.825.439 ◽

2013 ◽

Vol 825 ◽

pp. 439-442

Author(s):

Qian Qian Yu ◽

Emiko Morioka ◽

Tsuyoshi Hirajima ◽

Keiko Sasaki

Keyword(s):

Organic Matter ◽

Thermal Treatment ◽

Room Temperature ◽

Lithium Ion ◽

Microbial Transformations ◽

Mn Oxide ◽

Biogenic Mn Oxide ◽

Li Ion ◽

Mn Oxides ◽

Sorption Characteristics

Geomimetics, taking lessons from natures biogenic mineralization mechanisms, can provide powerful tools for advancing biohydrometallurgical processing. Microbial transformations are largely responsible for the Mn oxides found in nature. In this research biogenic birnessite was produced by a manganese-oxidizing fungus, Paraconiothyrium sp. WL-2, at pH 6.5 under room temperature, and characterized by XRD and TG-DTA. Abiotic (chemically synthesized) acidic birnessite was also prepared hydrometallurgically and subjected to a similar battery of characterization techniques. Following thermal treatment the sorption characteristics of these two materials were compared. The biogenic precursor showed several advantages to produce more effective Li-ion sieve than the chemically synthesized precursor. First, a shorter calcination period was required to produce Li4Mn5O12 without other phases; second, a greater content and higher crystallinity of H4Mn5O12 were obtained from the biogenic precursor. These advantages might be caused by poorer crystallinity and around 20 wt% organic matter in biogenic birnessite. While sorption density of Li+ in mmol/g was basically dependent on contents of H4Mn5O12 phase, the unique morphologies and sorption density were maintained with biogenic precursor even after repetition of sorption/desorption of Li+.

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Vibrational Spectroscopic Study for Qualitative Assessment of Mn-oxide Ore

Resource Geology ◽

10.1111/rge.12083 ◽

2015 ◽

Vol 66 (1) ◽

pp. 12-23 ◽

Cited By ~ 2

Author(s):

Swatirupa Pani ◽

Saroj K. Singh ◽

Birendra K. Mohapatra

Keyword(s):

Spectroscopic Study ◽

Qualitative Assessment ◽

Mn Oxide

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Direct Identification of a Bacterial Manganese(II) Oxidase, the Multicopper Oxidase MnxG, from Spores of Several Different Marine Bacillus Species

Applied and Environmental Microbiology ◽

10.1128/aem.01240-07 ◽

2007 ◽

Vol 74 (5) ◽

pp. 1527-1534 ◽

Cited By ~ 101

Author(s):

Gregory J. Dick ◽

Justin W. Torpey ◽

Terry J. Beveridge ◽

Bradley M. Tebo

Keyword(s):

Sodium Dodecyl ◽

Multicopper Oxidase ◽

Bacillus Species ◽

Direct Identification ◽

Mn Oxide ◽

Bacterial Enzyme ◽

Biogenic Mn Oxide ◽

Small Hydrophobic Protein ◽

High Concentrations ◽

Small Hydrophobic

ABSTRACT Microorganisms catalyze the formation of naturally occurring Mn oxides, but little is known about the biochemical mechanisms of this important biogeochemical process. We used tandem mass spectrometry to directly analyze the Mn(II)-oxidizing enzyme from marine Bacillus spores, identified as an Mn oxide band with an in-gel activity assay. Nine distinct peptides recovered from the Mn oxide band of two Bacillus species were unique to the multicopper oxidase MnxG, and one peptide was from the small hydrophobic protein MnxF. No other proteins were detected in the Mn oxide band, indicating that MnxG (or a MnxF/G complex) directly catalyzes biogenic Mn oxide formation. The Mn(II) oxidase was partially purified and found to be resistant to many proteases and active even at high concentrations of sodium dodecyl sulfate. Comparative analysis of the genes involved in Mn(II) oxidation from three diverse Bacillus species revealed a complement of conserved Cu-binding regions not present in well-characterized multicopper oxidases. Our results provide the first direct identification of a bacterial enzyme that catalyzes Mn(II) oxidation and suggest that MnxG catalyzes two sequential one-electron oxidations from Mn(II) to Mn(III) and from Mn(III) to Mn(IV), a novel type of reaction for a multicopper oxidase.

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Specific Sorption Behavior of Actinoids on Biogenic Mn Oxide

Chemistry Letters ◽

10.1246/cl.2011.806 ◽

2011 ◽

Vol 40 (8) ◽

pp. 806-807 ◽

Cited By ~ 7

Author(s):

Kazuya Tanaka ◽

Yukinori Tani ◽

Toshihiko Ohnuki

Keyword(s):

Sorption Behavior ◽

Mn Oxide ◽

Biogenic Mn Oxide

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Mn oxide formation by phototrophs: Spatial and temporal patterns, with evidence of an enzymatic superoxide-mediated pathway

Scientific Reports ◽

10.1038/s41598-019-54403-8 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 5

Author(s):

Dominique L. Chaput ◽

Alexandré J. Fowler ◽

Onyou Seo ◽

Kelly Duhn ◽

Colleen M. Hansel ◽

...

Keyword(s):

Oxygen Radical ◽

Extracellular Proteins ◽

Specific Time ◽

Oxide Formation ◽

Spatial And Temporal Patterns ◽

Time Points ◽

Mn Oxide ◽

Abiotic Oxidation ◽

Bacteria And Fungi ◽

Mn Oxides

AbstractManganese (Mn) oxide minerals influence the availability of organic carbon, nutrients and metals in the environment. Oxidation of Mn(II) to Mn(III/IV) oxides is largely promoted by the direct and indirect activity of microorganisms. Studies of biogenic Mn(II) oxidation have focused on bacteria and fungi, with phototrophic organisms (phototrophs) being generally overlooked. Here, we isolated phototrophs from Mn removal beds in Pennsylvania, USA, including fourteen Chlorophyta (green algae), three Bacillariophyta (diatoms) and one cyanobacterium, all of which consistently formed Mn(III/IV) oxides. Isolates produced cell-specific oxides (coating some cells but not others), diffuse biofilm oxides, and internal diatom-specific Mn-rich nodules. Phototrophic Mn(II) oxidation had been previously attributed to abiotic oxidation mediated by photosynthesis-driven pH increases, but we found a decoupling of Mn oxide formation and pH alteration in several cases. Furthermore, cell-free filtrates of some isolates produced Mn oxides at specific time points, but this activity was not induced by Mn(II). Manganese oxide formation in cell-free filtrates occurred via reaction with the oxygen radical superoxide produced by soluble extracellular proteins. Given the known widespread ability of phototrophs to produce superoxide, the contribution of phototrophs to Mn(II) oxidation in the environment may be greater and more nuanced than previously thought.

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