Isolation of Halotolerant Bacteria from Rhizosphere of Khewra Salt Mine Halophytes and their Application to Induce Salt Tolerance in Wheat

Abstract Characterization of the salt-tolerance function in microbial communities is important for understanding the soil and sediment biodiversity distribution near saltern affected by the environmental factors. Here, we combined high-throughput sequencing of 16S rDNA and ITS to investigate the distribution and salt-tolerance function of microbial communities in coastal soils and sediments present near Yinggehai saltern field of Hainan Island, China. The microbial communities in the soil and sediments of the land zone (YGHLS), the intertidal zone (YGHIS), and inshore zone (YGHWS) were compared. PCoA of weighted and unweighted UniFrac distances showed the difference in soil microbial communities across the samples. ANOSIM analysis revealed that the three samples were separated from each other. Halomonas , Halobacillus and Wallemia presented in the samples and accounted for 0.0335 ± 0.0586%, 0.0241 ± 0.0304% and 0.0308 ± 0.0445% of the total microbial community. Relative abundance of Na + :H + antiporter, Trk system potassium uptake protein and Kdp operon response regulator in the samples exceed 0.02%, 0.09% and 0.06% respectively. It revealed that the halotolerant bacteria of Yinggehai coastal soil and sediment mainly take the Trk system for salt-tolerance function.

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Enhancement of growth and salt tolerance of red pepper seedlings (Capsicum annuum L.) by regulating stress ethylene synthesis with halotolerant bacteria containing 1-aminocyclopropane-1-carboxylic acid deaminase activity

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2011.01.015 ◽

2011 ◽

Vol 49 (4) ◽

pp. 427-434 ◽

Cited By ~ 149

Author(s):

Md. Ashaduzzaman Siddikee ◽

Bernard R. Glick ◽

Puneet S. Chauhan ◽

Woo jong Yim ◽

Tongmin Sa

Keyword(s):

Carboxylic Acid ◽

Salt Tolerance ◽

Capsicum Annuum ◽

Red Pepper ◽

Ethylene Synthesis ◽

Halotolerant Bacteria ◽

Stress Ethylene ◽

Capsicum Annuum L

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Application of SSR Technique for the Identification of Markers Linked to Salinity Tolerance in Rice

Progressive Agriculture ◽

10.3329/pa.v19i2.16929 ◽

2013 ◽

Vol 19 (2) ◽

pp. 57-65

Author(s):

MH Kabir ◽

MM Islam ◽

SN Begum ◽

AC Manidas

Keyword(s):

Salt Tolerance ◽

Ssr Markers ◽

Salinity Tolerance ◽

Seedling Stage ◽

Phenotypic Screening ◽

Salt Tolerant ◽

Marker Assisted Breeding ◽

Hydroponic System ◽

Rice Landrace ◽

Salt Susceptible

A cross was made between high yielding salt susceptible BINA variety (Binadhan-5) with salt tolerant rice landrace (Harkuch) to identify salt tolerant rice lines. Thirty six F3 rice lines of Binadhan-5 x Harkuch were tested for salinity tolerance at the seedling stage in hydroponic system using nutrient solution. In F3 population, six lines were found as salt tolerant and 10 lines were moderately tolerant based on phenotypic screening at the seedling stage. Twelve SSR markers were used for parental survey and among them three polymorphic SSR markers viz., OSR34, RM443 and RM169 were selected to evaluate 26 F3 rice lines for salt tolerance. With respect to marker OSR34, 15 lines were identified as salt tolerant, 9 lines were susceptible and 2 lines were heterozygous. While RM443 identified 3 tolerant, 14 susceptible and 9 heterozygous rice lines. Eight tolerant, 11 susceptible and 7 heterozygous lines were identified with the marker RM169. Thus the tested markers could be efficiently used for tagging salt tolerant genes in marker-assisted breeding programme.DOI: http://dx.doi.org/10.3329/pa.v19i2.16929 Progress. Agric. 19(2): 57 - 65, 2008

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