Principal component analysis and assessment of Brassica napus L. accessions for salt tolerance using stress tolerance indices

2020 ◽  
Vol 53 (1) ◽  
Author(s):  
Shamsa Kanwal ◽  
M. Hammad Nadeem Tahir ◽  
Humera Razzaq
Keyword(s):  
Principal Component Analysis ◽  
Brassica Napus ◽  
Salt Tolerance ◽  
Stress Tolerance ◽  
Principal Component ◽  
Component Analysis ◽  
Brassica Napus L ◽  
Tolerance Indices

scholarly journals Using Principal Component Analysis and RNA-Seq to Identify Candidate Genes That Control Salt Tolerance in Garlic (Allium sativum L.)

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 691
Author(s):  
Xiangjun Zhou ◽  
Yanxia Dou ◽  
Xiaoxia Huang ◽  
Gang Li ◽  
Hongrui Zhang ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Salt Tolerance ◽  
Candidate Genes ◽  
Principal Component ◽  
Dry Weight ◽  
Component Analysis ◽  
Salt Tolerant ◽  
Fresh Weight ◽  
Shoot Dry Weight ◽  
Leaves And Roots

To examine physiological responses of garlic to salinity, 17-day-old seedlings of eight soft-neck accessions were treated with 200 mM NaCl for seven days in a hydroponic system. Several morphological and physiological traits were measured at the end of the treatment, including shoot height, shoot fresh weight, shoot dry weight, root length, root fresh weight, root dry weight, photosynthesis rate, and concentrations of Na+ and K+ in leaves. The principal component analysis showed that shoot dry weight and K+/Na+ ratio contribute the most to salt tolerance among the garlic accessions. As a result, salt-tolerant and sensitive accessions were grouped based on these two parameters. Furthermore, to investigate the molecular mechanisms in garlic in response to salinity, the transcriptomes of leaves and roots between salt-tolerant and salt-sensitive garlic accessions were compared. Approximately 1.5 billion read pairs were obtained from 24 libraries generated from the leaves and roots of the salt-tolerant and salt-sensitive garlic accessions. A total of 47,509 genes were identified by mapping the cleaned reads to the garlic reference genome. Statistical analysis indicated that 1282 and 1068 genes were upregulated solely in the tolerant leaves and roots, whereas 1505 and 1203 genes were downregulated exclusively in the tolerant leaves and roots after NaCl treatment, respectively. Functional categorization of these genes revealed their involvement in a variety of biological processes. Several genes important for carotenoid biosynthesis, auxin signaling, and K+ transport were strongly altered in roots by NaCl treatment and could be candidate genes for garlic salt tolerance improvement.

scholarly journals Multivariate Analysis as a Tool for Studying the Effects of Salinity in Different Melon Landraces at Germination Stage

2016 ◽  
Vol 44 (1) ◽  
pp. 264-271 ◽  
Author(s):  
Behrooz SARABI ◽  
Sahebali BOLANDNAZAR ◽  
Nasser GHADERI ◽  
Seyed Jalal TABATABAEI
Keyword(s):  
Principal Component Analysis ◽  
Salt Tolerance ◽  
Seed Germination ◽  
Seedling Growth ◽  
Principal Component ◽  
Component Analysis ◽  
Growth Stages ◽  
Tolerance Index ◽  
Plant Seed ◽  
Salt Tolerance Index

Although salt stress affects all growth stages of a plant, seed germination and seedling growth stages are known to be more sensitive for most plant species. The current research was carried out to find the most effective indicators when screening melons for salt tolerance. For this purpose, a factorial experiment as a completely randomized design was performed in order to investigate the effects of salinity (0, 20, 40, 60 and 80 mM NaCl) on seed germination parameters, hydrogen peroxide content, lipid peroxidation and some antioxidant enzymes (guaiacol peroxidase, ascorbate peroxidase and superoxide dismutase) in five Iranian melon landraces and one melon cultivar ‘Galia’ F1 at early seedling growth stage. The ‘Galia’ F1 was included for comparison as a salt-tolerant cultivar. The data collected from all salinity treatments across six landraces were used for analysis of variance and principal component analysis (PCA) using SPSS software as well as for obtaining Pearson’s correlation coefficients (r) among all physiological parameters. Obtained results indicated that averaged all landraces across all treatments, increased NaCl significantly affected all the parameters measured. In all landraces, salt tolerance index (STI) decreased as the salt concentrations increased. The principal component analysis revealed variations among the traits and determined four main factors that explained 97.53% of the total variance. Simple correlation coefficient analyses showed the existence of significant positive and negative correlations among characteristics. Our study suggests that the salt tolerance index and principal component analysis can be used as accurate and easy indicators when screening melons for salt tolerance.

scholarly journals Evaluation of Cold Tolerance in Rapeseed (Brassica napus L.) Using Stress Tolerance Indices

2019 ◽  
Vol 9 (2) ◽  
pp. 13-28
Author(s):  
H. Amiri oghan ◽  
R. Ataei ◽  
M. Gholamhosseini ◽  
B. Alizadeh ◽  
◽  
...  
Keyword(s):  
Brassica Napus ◽  
Stress Tolerance ◽  
Cold Tolerance ◽  
Brassica Napus L ◽  
Tolerance Indices

scholarly journals Assessment of watermelon accessions for salt tolerance using stress tolerance indices

2017 ◽  
Vol 41 (6) ◽  
pp. 616-625 ◽  
Author(s):  
Ercan Ekbic ◽  
Cagri Cagıran ◽  
Kursat Korkmaz ◽  
Malik Arsal Kose ◽  
Veysel Aras
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Stress Tolerance ◽  
Geometric Mean ◽  
Principal Component ◽  
Saline Stress ◽  
Tolerance Index ◽  
Salt Tolerant ◽  
Starting Point ◽  
Tolerance Indices

ABSTRACT Salt stress is the most significant constraint for agricultural production in arid and semi-arid regions. Thus, genetically improved stress-tolerant varieties are needed for the future. The identification of salt-tolerant genotypes is the starting point for such breeding studies. This study was conducted to determine and assess the tolerance of different watermelon genotypes under saline conditions. Twenty-two watermelon genotypes and accessions were grown in pots with 3 kg of soil in four saline stress conditions (0 mmol kg-1 as the control, 25, 50 and 100 mmol kg-1 NaCl). The detrimental effects of salt stress on the plants were evident with increasing doses of NaCl. Stress indices calculated over the plant dry weights under the 100 mmol kg-1 salinity level were used to assess the salt tolerance of the genotypes. Stress intensity was calculated as 0.76. Such a value indicated that the highest dose of salt exerted severe stress on the plants. The G04, G14 and G21 genotypes were considered to be salt tolerant, since these genotypes showed the highest values of K/Na and Ca/Na ratios in the plant tissue. The losses in dry mass at severe salt stress reached 75.48%. In principal component analyses, the genotypes had positive correlations with stress tolerance indices of MP (mean productivity), GMP (geometric mean productivity) and STI (stress tolerance index). The GMP and STI indices indicated that G04 (a member of Citrullus colocynthis), G14 and G21 could be prominent sources to develop salt tolerance.

A German version of the Intermittent Claudication Questionnaire (ICQ): cultural adaptation and validation

VASA ◽  
2012 ◽  
Vol 41 (5) ◽  
pp. 333-342 ◽  
Author(s):  
Kirchberger ◽  
Finger ◽  
Müller-Bühl
Keyword(s):  
Principal Component Analysis ◽  
Intermittent Claudication ◽  
Completion Time ◽  
Short Form ◽  
Principal Component ◽  
Component Analysis ◽  
German Version ◽  
Average Completion Time ◽  
Sf 36 ◽  
Related Quality

Background: The Intermittent Claudication Questionnaire (ICQ) is a short questionnaire for the assessment of health-related quality of life (HRQOL) in patients with intermittent claudication (IC). The objective of this study was to translate the ICQ into German and to investigate the psychometric properties of the German ICQ version in patients with IC. Patients and methods: The original English version was translated using a forward-backward method. The resulting German version was reviewed by the author of the original version and an experienced clinician. Finally, it was tested for clarity with 5 German patients with IC. A sample of 81 patients were administered the German ICQ. The sample consisted of 58.0 % male patients with a median age of 71 years and a median IC duration of 36 months. Test of feasibility included completeness of questionnaires, completion time, and ratings of clarity, length and relevance. Reliability was assessed through a retest in 13 patients at 14 days, and analysis of Cronbach’s alpha for internal consistency. Construct validity was investigated using principal component analysis. Concurrent validity was assessed by correlating the ICQ scores with the Short Form 36 Health Survey (SF-36) as well as clinical measures. Results: The ICQ was completely filled in by 73 subjects (90.1 %) with an average completion time of 6.3 minutes. Cronbach’s alpha coefficient reached 0.75. Intra-class correlation for test-retest reliability was r = 0.88. Principal component analysis resulted in a 3 factor solution. The first factor explained 51.5 of the total variation and all items had loadings of at least 0.65 on it. The ICQ was significantly associated with the SF-36 and treadmill-walking distances whereas no association was found for resting ABPI. Conclusions: The German version of the ICQ demonstrated good feasibility, satisfactory reliability and good validity. Responsiveness should be investigated in further validation studies.

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