Abstract
Background
Experimental studies have determined the chemopreventive effects of vitamin D against theesophageal squamous cell carcinoma (ESCC); however, results from the epidemiological studies arenot yet well established. The current study aimed to evaluate the associations between plasmavitamin D levels and variants on vitamin D metabolic-related genes with the risks for ESCC.
Methods
A hospital-based case-control study was performed. 670 ESCC patients and 598 controls were recruited in a Northern Chinese population. The vitamin D levels in the plasma were determined with the ELISA detection kits provided by R&D Company and following the manufacturer's instructions. The coefficients of variation for the 25(OH)D levels between-assay and within-assay were less than 12.5%. Common variants on vitamin D metabolism-related genes CYP24A1, DHCR7, GC, CYP27B1, and vitamin D receptor (VDR) and theplasma 25(OH)D level were determined. The genomic DNA was extracted from the blood lymphocyte cells with the QIAamp DNA Mini Kit (Qiagen, Germany). The SNPs were genotyped using the GenomeLab SNPstream 12-plex Genotyping System (Beckman Coulter, Inc., Fullerton, CA) following the manufacturer's instructions. The unconditional logistic regression method was applied to determine the associations between the variants and vitamin D level and ESCC.
Results
we evaluated 6 common variants on genes related to vitamin D metabolism including DHCR7 (rs3829251), DBP (rs12512631 and rs115563), CYP24A1 (rs2296241), CYP27B1 (rs4646536) and VDR (rs11568820) and their association with ESCC risk. For rs2296241, rs11568820 and rs4646536, interaction analysis shows that higher the plasma 25(OH)D levels are associated with a decreased ESCC risk.For rs3829251, rs3829251 and rs1155563, the plasma 25(OH)D levels did not vary across genotypes for any of the polymorphisms. The variants rs2296241 on CYP24A1 and rs11568820 on VDR are significantly associated with ESCC cancer.
Conclusion
Vitamin D signaling pathways may participate in the ESCC development. Further studies with larger sample size are warranted to confirm the results. Intervention studies are needed to determine whether vitamin D supplementation may reduce the ESCC risk in the Chinese population.
Disclosure
All authors have declared no conflicts of interest.
Association of NAD(P)H: quinone oxidoreductase 1 (NQO1) C609T polymorphism with esophageal squamous cell carcinoma in a German Caucasian and a northern Chinese population
