Optimization and characterization of Brimonidine tartrate nanoparticles-loaded in situ gel for the treatment of glaucoma

2021 ◽  
Author(s):  
Pankaj Kumar Sharma ◽  
Meenakshi Kanwar Chauhan
Keyword(s):  
In Situ Gel ◽  
Brimonidine Tartrate

scholarly journals DEVELOPMENT AND CHARACTERIZATION OF IN SITU GEL OF XANTHAN GUM FOR OPHTHALMIC FORMULATION CONTAINING BRIMONIDINE TARTRATE

2018 ◽  
Vol 11 (7) ◽  
pp. 277 ◽  
Author(s):  
Vazir Ashfaq Ahmed ◽  
Divakar Goli
Keyword(s):  
Drug Release ◽  
Xanthan Gum ◽  
Gelation Time ◽  
Gel Strength ◽  
Eye Drops ◽  
In Situ Gel ◽  
23 Factorial Design ◽  
Brimonidine Tartrate

Objective: The goal of this study was to develop and characterize an ion-activated in situ gel-forming brimonidine tartrate, solution eye drops containing xanthan gum as a mucoadhesive polymer.Method: Sol-gel formulation was prepared using gellan gum as an ion-activated gel-forming polymer, xanthan gum as mucoadhesive agent, and hydroxypropyl methyl cellulose (HPMC E50LV) as release retardant polymer. Phenylethyl alcohol is used as preservatives in borate buffer. The 23 factorial design was employed to optimize the formulation considering the concentration of gelrite, xanthan gum and HPMC as independent variables, gelation time, gel strength, and mucoadhesive force (N). Gelation time , gel strength, mucoadhesive force (N), viscosity (cP) and in vitro percentage drug release were chosen as dependent variables. The formulation was characteristics for pH, clarity, isotonicity, sterility, rheological behavior, and in vitro drug release, ocular irritation, and ocular visualization.Result: Based on desirability index of responses, the formulation containing a concentration of gelrite (0.4%), xanthan gum (0.21%), and HPMC (HPMC E50 (0.24%) was found to be the optimized formulation concentration developed by 23 factorial design. The solution eye drops resulted in an in situ phase change to gel-state when mixed with simulated tear fluid. The gel formation was also confirmed by viscoelastic measurements. Drug release from the gel followed non-fickian mechanism with 88% of drug released in 10 h, thus increased the residence time of the drug.Conclusion: An in situ gelling system is a valuable alternative to the conventional system with added benefits of sustained drug release which may ultimately result in improved patient compliance.

scholarly journals Novel Approach for Design and Characterization Of Muco Adhesive Buccal Ganciclovir In Situ Gel

2020 ◽  
Vol 8 (3) ◽  
pp. 19-33
Author(s):  
CH.N.V.S Masthan Rao ◽  
R.B. Desireddy ◽  
D. Vasavilatha ◽  
G.L.S. Mounika ◽  
G. Ramya Sadhana ◽  
...  
Keyword(s):  
In Situ Gel ◽  
Novel Approach

scholarly journals PHYSICAL CHARACTERIZATION OF IN SITU OPHTHALMIC GEL: A CONCISE REVIEW

2022 ◽  
pp. 18-21
Author(s):  
INSAN SUNAN KURNIAWANSYAH ◽  
IYAN SOPYAN ◽  
GENI REFSI
Keyword(s):  
Therapeutic Effect ◽  
Contact Time ◽  
Drug Preparation ◽  
Journal Review ◽  
In Situ Gel ◽  
Concise Review ◽  
Gel Formulations ◽  
Active Substances

In situ ophthalmic gel is a type of eye drug preparation that has a higher bioavailability value and has a longer contact time with maximum therapeutic effect and with minimal side effects compared to conventional eye preparations. The preparation of ophthalmic in situ gel is required characterization to make sure that the prepared preparations meet the standards and are safe when used. This journal review aims to look at the methods used in characterizing physical properties in in situ ophthalmic gel formulations with different active substances such as rheology studies, organoleptic tests, pH, clarity, and gelling capacity. In order to get the best formulation of in situ ophthalmic gel preparations so as to provide maximum therapeutic effect.

scholarly journals Preparation and detailed characterization of fusidic acid loaded in situ gel formulations for ophthalmic application

2021 ◽  
Vol 25 (1) ◽  
pp. 1-1
Author(s):  
Seda RENÇBER ◽  
Ece ÖZCAN BÜLBÜL ◽  
Neslihan ÜSTÜNDAĞ OKUR ◽  
Zeynep AY ŞENYİĞİT
Keyword(s):  
Fusidic Acid ◽  
Detailed Characterization ◽  
In Situ Gel ◽  
Gel Formulations

scholarly journals Formulation and Characterization of Tranylcypromine loaded Polymeric Micellar In-Situ Nasal Gel for treatment of Depression

2020 ◽  
pp. 149-165
Keyword(s):  
Polymeric Micelles ◽  
Research Work ◽  
In Vitro Release ◽  
Polymeric Micelle ◽  
In Situ Gel ◽  
Treatment Of Depression ◽  
In Situ Nasal Gel

:Tranylcypromine is a drug used as antidepressant,anxiolytic, nonselective MAO A/B inhibitor. This drug is used to treat depression.The research was conducted to develop a polymeric micelle using a block copolymer, Pluronic F-68 and Gelucire 50/13 to improve the permeability of Tranylcypromine (TCP). A direct dissolution method was used to prepare polymeric micelles. The prepared micelles were characterised for particle size, % EE, zeta potential, in-vitro release. These micelles solution was used to prepare in situ gel by cold method in order to achieve controlled release. Central composite design was used for optimization of both polymeric micelles and insitu nasal gel.The main objective of this research work is to develop formulation acting centrally without undergoing first pass metabolism i.ie. directly nasal to brain delivery route.

scholarly journals Development and Characterization of Voriconazole Loaded In Situ Gel Formulations for Ophthalmic Application

2016 ◽  
Vol 13 (3) ◽  
pp. 311-317 ◽  
Author(s):  
Neslihan ÜSTÜNDAĞ OKUR ◽  
Ayşegül YOLTAŞ ◽  
Vildan YOZGATLI
Keyword(s):  
In Situ Gel ◽  
Gel Formulations

scholarly journals Amsacta moorei Entomopoxvirus Expresses an Active Superoxide Dismutase

2004 ◽  
Vol 78 (19) ◽  
pp. 10265-10275 ◽  
Author(s):  
M. N. Becker ◽  
W. B. Greenleaf ◽  
D. A. Ostrov ◽  
R. W. Moyer
Keyword(s):  
Superoxide Dismutase ◽  
Metal Ion ◽  
Metal Ion Binding ◽  
In Situ Gel ◽  
Reading Frame ◽  
Catalytically Active ◽  
Infected Cells ◽  
Group B

ABSTRACT The entomopoxvirus from Amsacta moorei serves as the prototype of the group B entomopoxviruses. One of the interesting genes found in Amsacta moorei entomopoxvirus (AmEPV) is a superoxide dismutase (sod) (open reading frame AMV255). Superoxide dismutases (SODs) catalyze the conversion of superoxide radicals to hydrogen peroxide and oxygen. Many vertebrate poxviruses contain a sod gene, but to date, none have been demonstrated to be active. There are three families of SODs, characterized by their metal ion-binding partners, Fe, Mn, or Cu and Zn. Poxvirus enzymes belong to the Cu-Zn SOD family. Unlike inactive vertebrate poxvirus SODs, AMVSOD contains all the amino acids necessary for function. We expressed and purified a 6X-His-tagged version of the AMVSOD in Escherichia coli. The recombinant AMVSOD demonstrates superoxide dismutase activity both in an in situ gel assay and by stopped flow spectrophotometry. The k cat/K m for AMVSOD is 4 × 107 M−1s−1. In infected cells, the AMVSOD protein behaves as a dimer and is catalytically active; however, disruption of the gene in AMEPV has little or no effect on growth of the virus in cell culture. An analysis of mRNA expression indicates that AMVsod is expressed late during infection of Lymantria dispar (Ld652) cells and produces a discrete nonpolydisperse transcript. Characterization of protein expression with a monoclonal antibody generated against AMVSOD confirms that the AMVSOD protein can be classified as a late, postreplicative gene. Therefore, AMVSOD is the first example of an active poxvirus SOD.

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