scholarly journals Reliability of morphological criteria for sexing birds during ringing, assessed using molecular methods – a study of thirteen species of passerines and near passerines

2022 ◽  
pp. 1-11
Author(s):  
Lucy D. Rowley ◽  
Marcial Felgueiras ◽  
Guillaume Réthoré ◽  
Filipa Bragança ◽  
Robert J. Thomas ◽  
...  
Keyword(s):  
Molecular Methods ◽  
Morphological Criteria

scholarly journals Molecular Methods for the Detection of Natural Hybrids in Sturgeon Populations

2015 ◽  
Vol 16 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Alexandru Burcea ◽  
Iulia Elena Florescu ◽  
Andreea Dudu ◽  
Sergiu Emil Georgescu ◽  
Marieta Costache
Keyword(s):  
Mitochondrial Dna ◽  
Dna Markers ◽  
Molecular Methods ◽  
Hybrid Species ◽  
Habitat Area ◽  
Natural Hybrids ◽  
Sturgeon Species ◽  
Morphological Criteria ◽  
Hybrid Sturgeon

Abstract Due to construction of the Iron Gates dams, the Lower Danube has suffered a decrease in sturgeon populations. The dams have decreased sturgeon habitat area, which in turn has caused an overlap of reproduction areas for all sturgeon species. The ease with which sturgeon species can create hybrid offsprings gave rise to an increase in the number of hybrid sturgeon species now found in the Lower Danube area. We propose a set of molecular methods for hybrid species using DNA markers represented by microsatellites and mitochondrial DNA. This identification data and methodology is important for use on sturgeon farms due to the need to correctly identify species of sturgeons. Using the proposed methodologies, it is possible to avoid identification errors that might appear when using only morphological criteria to idenfy sturgeons

scholarly journals Echinococcus multilocularis and Echinococcus granulosus in canines in North-Khorasan Province, northeastern Iran, identified using morphology and genetic characterization of mitochondrial DNA

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Zahra Heidari ◽  
Mitra Sharbatkhori ◽  
Iraj Mobedi ◽  
Seyed Hossein Mirhendi ◽  
Bahram Nikmanesh ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Echinococcus Granulosus ◽  
Echinococcus Multilocularis ◽  
Genetic Characterization ◽  
Comparative Sequence Analysis ◽  
Molecular Methods ◽  
Morphological Description ◽  
Intraspecific Divergence ◽  
Morphological Criteria

Abstract Background Canids are definitive hosts of Echinococcus multilocularis and Echinococcus granulosus. This study aimed to survey these two Echinococcus species in canids of North-Khorasan Province, northeastern Iran, using morphological criteria and genetic characterization of mitochondrial DNA. Methods The carcasses of 106 canids, namely 61 jackals (Canis aureus), 23 foxes (Vulpes vulpes), 19 dogs (Canis familiaris) and three wolves (Canis lupus) were collected from the study area in 2013–2014 and examined for Echinococcus species. Morphological features were assessed by microscopy of adult worms. For molecular characterization, DNA was extracted, mostly from the adult worms but also from eggs. DNA fragments of the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) mitochondrial genes were amplified and sequenced. Sequences were aligned and compared with reference sequences. Intraspecific and interspecific diversity were calculated and phylogenetic analysis was performed. Results Overall, 9.4% of the canids (eight jackals and two foxes) were found infected with E. multilocularis by molecular methods, of which seven cases were also confirmed using morphological description of the adult worms. Echinococcus granulosus was found in 6.6% of the canines (four dogs, two jackals and one wolf) as determined by both molecular methods and adult cestode morphology. All E. granulosus isolates were identified as the G1 genotype. Comparative sequence analysis indicated 0–0.7% and 0% intraspecific divergence within E. granulosus isolates and 0% and 0–0.2% within E. multilocularis isolates for cox1 and nad1, respectively. Conclusions This study revealed the presence of E. multilocularis and E. granulosus in canids of North-Khorasan Province of Iran. Jackals were found infected with both E. multilocularis and E. granulosus, but infection with the former species was higher.

Ultrastructural study on the development of rat amygdaloid body

1990 ◽  
Vol 48 (3) ◽  
pp. 432-433
Author(s):  
A. Manolova ◽  
S. Manolov
Keyword(s):  
Nerve Cells ◽  
Amygdaloid Complex ◽  
Amygdaloid Body ◽  
Thin Ring ◽  
Morphological Criteria ◽  
Neural Processes ◽  
Abundant Cytoplasm ◽  
Level 1 ◽  
Few Data ◽  
Oval Shape

Relatively few data on the development of the amygdaloid complex are available only at the light microscopic level (1-3). The existence of just general morphological criteria requires the performance of other investigations in particular ultrastructural in order to obtain new and more detailed information about the changes in the amygdaloid complex during development.The prenatal and postnatal development of rat amygdaloid complex beginning from the 12th embrionic day (ED) till the 33rd postnatal day (PD) has been studied. During the early stages of neurogenesis (12ED), the nerve cells were observed to be closely packed, small-sized, with oval shape. A thin ring of cytoplasm surrounded their large nuclei, their nucleoli being very active with various size and form (Fig.1). Some cells possessed more abundant cytoplasm. The perikarya were extremely rich in free ribosomes. Single sacs of the rough endoplasmic reticulum and mitochondria were observed among them. The mitochondria were with light matrix and possessed few cristae. Neural processes were viewed to sprout from some nerve cells (Fig.2). Later the nuclei were still comparatively large and with various shape.

NEWER MOLECULAR METHODS FOR THE SURVEILLANCE OF ANTIMALARIAL RESISTANCEBY USING NON-INVASIVE SAMPLES

2020 ◽  
Vol 23 (13) ◽  
Author(s):  
Abisha Jayasingh Chellammal ◽  
Vasanthi Rompicherla ◽  
Jayanthi Subramaniam ◽  
Priyadarshini Shanmugam
Keyword(s):  
Molecular Methods ◽  
Non Invasive

MORPHOLOGICAL CRITERIA OF REMOTENESS OF ISCHEMIC STROKES’ DEVELOPMENT INTO LATE SUBACUTE AND CHRONIC STAGES

2017 ◽  
Author(s):  
P. A. Machinskiy ◽  
◽  
N. A. Plotnikova ◽  
S. P. Kemaykin ◽  
A. G. Rybakov ◽  
...  
Keyword(s):  
Morphological Criteria

scholarly journals Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Ana Mafalda Dordio ◽  
Relja Beck ◽  
Telmo Nunes ◽  
Isabel Pereira da Fonseca ◽  
Jacinto Gomes
Keyword(s):  
Clinical Status ◽  
Molecular Methods ◽  
Hepatozoon Canis ◽  
Clinical Approach ◽  
Limited Information ◽  
Vector Borne Diseases ◽  
Wide Range ◽  
Other Information ◽  
Southern Portugal ◽  
Vector Borne

Abstract Background Canine vector-borne diseases (CVBDs) are caused by a wide range of pathogens transmitted by arthropods. They have been an issue of growing importance in recent years; however, there is limited information about the vector-borne pathogens circulating in Portugal. The aim of the present study was to detect canine vector-borne bacteria and protozoa of veterinary and zoonotic importance using molecular methods. Methods One hundred and forty-two dogs from Lisbon, southern Portugal, were tested: 48 dogs from a veterinary hospital clinically suspected of vector-borne diseases and 94 apparently healthy dogs from shelters. Anaplasma spp./Ehrlichia spp., Babesia/Theileria spp., Hepatozoon spp., and Mycoplasma spp. infections were detected by PCR from blood samples and examined under light microscopy. Other information including clinical status and diagnostic test results were collected for each animal. Results Infections were detected by PCR in 48 (33.80%) dogs. Single infections were found in 35 dogs (24.64%), and co-infections were found in 13 (9.15%) dogs. Twenty-nine (20.42%) dogs were positive for Hepatozoon spp., 15 (10.56%) for Mycoplasma spp., 11 (7.75%) for Anaplasma spp./Ehrlichia spp., and six (4.21%) for Babesia spp. DNA sequencing was used to identify Babesia vogeli (2.81%), Babesia canis (1.40%), Hepatozoon canis (20.42%), Mycoplasma haematoparvum (2.11%), Mycoplasma haemocanis (8.45%), Anaplasma platys (7.04%), and Ehrlichia canis (0.70%). Conclusions This is the first molecular identification of B. canis and M. haematoparvum in dogs from southern Portugal. This study highlights the importance of molecular methods to identify CVBD pathogens in endemic areas and helps to guide the clinical approach of veterinarians in practice.

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