Antibacterial activity of herbal extracts against five plant pathogenic bacteria

Background: In the past few decades focus of research has been toward herbal medicines because of growing bacterial resistance and side effects of antimicrobial agents. The extract derived from the plants may increase the efficacy of antibiotics when used in combination against pathogenic bacteria. In the current study, the synergistic antibacterial efficacy of plant extracts in combination with antibiotics has been assessed on selected periodontal pathogens. Methods: Ethanolic extracts were prepared from Salvadora persica (Miswak) and Cinnamomum zeylanicum (Ceylon cinnamon), by the soxhalate method. Plaque samples were collected from clinical periodontitis patients to isolate and grow the periodontal pathobionts under favorable conditions. Susceptibility of bacteria to the extracts was assessed by gauging the diameter of the inhibition zones. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extracts were determined against each bacterium. Synergistic activity of plants extract in combination with antibiotics against the bacteria was also assessed by measuring the diameter of the inhibition zones. Results: Ethanolic extract of both the plants showed an inhibitory effect on the proliferation and growth of all four strains of periodontal pathobionts. Maximum antibacterial activity was exhibited by C. zeylanicum against Tannerella forsythia (MIC = 1.56 ± 0.24 mg/mL, MBC = 6.25 ± 0.68 mg/mL), whereas among all the studied groups the minimum activity was reported by C. zeylanicum against Aggregatibacter actinomycetemcomitans the (MIC = 12.5 ± 3.25 mg/mL, MBC = 75 ± 8.23 mg/mL). Combination of herbal extracts with different antibiotics revealed a synergistic antibacterial effect. The best synergism was exhibited by S. persica with metronidazole against A. actinomycetemcomitans (27 ± 1.78). Conclusions: Current in vitro study showed variable antibacterial activity by experimented herbal extracts against periodontal pathobionts. The synergistic test showed significant antibacterial activity when plant extracts were combined with antibiotics.

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Gc-Ms Analysis and Antibacterial Activity of Mangosteen Leaf Extracts against Plant Pathogenic Bacteria

American Journal of Plant Sciences ◽

10.4236/ajps.2016.77096 ◽

2016 ◽

Vol 07 (07) ◽

pp. 1013-1020 ◽

Cited By ~ 4

Author(s):

Qamar Mohammed Naji Alsultan ◽

Kamaruzaman Sijam ◽

Tavga Sulaiman Rashid ◽

Khairulmazmi Bin Ahmad

Keyword(s):

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Leaf Extracts ◽

Plant Pathogenic Bacteria ◽

Ms Analysis

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Antibacterial activity of the essential oil and extracts of Satureja hortensis against plant pathogenic bacteria and their potential use as seed disinfectants

Scientia Horticulturae ◽

10.1016/j.scienta.2013.01.027 ◽

2013 ◽

Vol 153 ◽

pp. 34-41 ◽

Cited By ~ 22

Author(s):

Recep Kotan ◽

Fatih Dadasoğlu ◽

Kenan Karagoz ◽

Ahmet Cakir ◽

Hakan Ozer ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Pathogenic Bacteria ◽

Satureja Hortensis ◽

Plant Pathogenic Bacteria ◽

Potential Use

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Antibacterial activity of caffeine against plant pathogenic bacteria

Acta Biochimica Polonica ◽

10.18388/abp.2015_1092 ◽

2015 ◽

Vol 62 (3) ◽

pp. 605-612 ◽

Cited By ~ 17

Author(s):

Wojciech Sledz ◽

Emilia Los ◽

Agnieszka Paczek ◽

Jacek Rischka ◽

Agata Motyka ◽

...

Keyword(s):

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Plant Pathogenic Bacteria

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The Antibacterial Activity of Chitosan Products Blended with Monoterpenes and Their Biofilms against Plant Pathogenic Bacteria

Scientifica ◽

10.1155/2016/1796256 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10 ◽

Cited By ~ 8

Author(s):

Mohamed E. I. Badawy ◽

Entsar I. Rabea ◽

Nehad E. M. Taktak ◽

Mahmoud A. M. El-Nouby

Keyword(s):

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Biological Activities ◽

Average Molecular Weight ◽

Erwinia Carotovora ◽

Food Protection ◽

Plant Pathogenic Bacteria ◽

Agar Dilution ◽

Microbial Attack

This study focuses on the biological activities of eleven chitosan products with a viscosity-average molecular weight ranging from 22 to 846 kDa in combination with the most active monoterpenes (geraniol and thymol), out of 10 tested, against four plant pathogenic bacteria,Agrobacterium tumefaciens,Erwinia carotovora,Corynebacterium fascians, andPseudomonas solanacearum. The antibacterial activity was evaluatedin vitroby the agar dilution technique as a minimum inhibitory concentration (MIC) that was found to be dependent on the type of the microorganism tested. The most active product of chitosan was used for biofilm production enriched with geraniol and thymol (0.1 and 0.5%) and the films were also evaluated against the tested bacteria. The biological bioactivities summarized here may provide novel insights into the functions of chitosan and some monoterpenes and potentially allow their use for food protection from microbial attack.

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