The anti-viral studies of plant extracts of Bunium persicum and Zataria multiflora on Nicotiana glutinosa and N. tabacum against tobacco mosaic virus (TMV; Tobamovirus, Virgaviridae) in Iran

Author(s):  
Samaneh Samavat ◽  
Vahid Ghelichi Zokhano
1955 ◽  
Vol 1 (7) ◽  
pp. 549-559 ◽  
Author(s):  
M. Weintraub ◽  
W. G. Kemp

A number of heterocyclic and miscellaneous organic compounds have been tested for their effectiveness as virus inhibitors by a half-leaf technique using Nicotiana glutinosa and tobacco mosaic virus. Several of these compounds were found to be effective in varying degrees, the main effects being a reduction in the total number of lesions produced, a delay in symptom expression, and a decrease in virus multiplication as indicated by small lesions, on the treated half-leaves. Although nothing is known about the mechanism of inhibition, it can be concluded that to effect inhibition compounds act through the physiology of the host, rather than directly on the virus. The virus content of treated half-leaves has been estimated by measuring their rate of oxygen consumption. These data indicate that the inhibiting compounds affect the metabolism of the host, as reflected in a change in respiration when compared to control half-leaves, and that the symptomless areas on the treated half-leaves do not contain significant concentrations of virus.


Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 65 ◽  
Author(s):  
Ya-Han Chen ◽  
Dong-Sheng Guo ◽  
Mei-Huan Lu ◽  
Jian-Ying Yue ◽  
Yan Liu ◽  
...  

The coumarin compound of osthole was extracted from Cnidium monnieri and identified by LC-MS and 1H- and 13C-NMR. Osthole was tested for anti-virus activity against tobacco mosaic virus (TMV) using the half-leaf method. The results showed that stronger antiviral activity on TMV infection appeared in Nicotiana glutinosa than that of eugenol and ningnanmycin, with inhibitory, protective, and curative effects of 72.57%, 70.26%, and 61.97%, respectively. Through observation of the TMV particles, we found that osthole could directly affect the viral particles. Correspondingly, the level of coat protein detected by Western blot was significantly reduced when the concentrations of osthole increased in tested plants compared to that of the control. These results suggest that osthole has anti-TMV activity and may be used as a biological reagent to control the plant virus in the half-leaf method.


1974 ◽  
Vol 52 (1) ◽  
pp. 23-25 ◽  
Author(s):  
Mary Boxall ◽  
B. H. MacNeill

The integrity of the local-lesion technique in producing genetically homogeneous isolates of tobacco mosaic virus from Nicotiana glutinosa has been verified using strains doubly labeled with genetic markers governing symptomatology and host range.


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