Binding and detoxification of chlorpyrifos by lactic acid bacteria on rice straw silage fermentation

The selection of lactic acid bacteria for use as silage additives is normally based upon their ability to dominate the silage fermentation and not upon benefits in animal performance. The object of this study was to investigate whether two lactic acid bacteria selected for fermentation characteristics would support the same animal performance as an established silage inoculant.On 5 June 1989, first cut, predominantly perennial ryegrass (Lolium perenne) was ensiled direct with no wilting in plastolene silos of 2 tonne capacity. The herbage was harvested with a precision chop forage harvester and the following additive treatments were applied: None, control (C); Formic acid (Add-F, BP Nutrition, 850g/kg; 3 litres/tonne), (F); inoculant E. (Ecosyl, ICI Pic, 106Lactobacillus plantarum /g): inoculant A, (Pediococcus sp, 10 /g); inoculant B (L. plantarum 106 /g). The silages were fed to 6 wether sheep (Suffolk x Halfbred), initial live-weight, 40.1 kg (s.d. 2.22kg). Because the silages were unstable aerobically and restricted quantities of each silage were available, all sheep were fed the silages in the same (random) order.

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Effects of adding a Lactobacillus Plantarum inoculant to grass ensiled at different dry matter concentrations and offered to beef cattle

Proceedings of the British Society of Animal Production (1972) ◽

10.1017/s0308229600026623 ◽

1994 ◽

Vol 1994 ◽

pp. 115-115 ◽

Cited By ~ 1

Author(s):

P. O'Kiely

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Beef Cattle ◽

Lactobacillus Plantarum ◽

Dry Matter ◽

Nutritive Value ◽

Silage Fermentation ◽

Different Levels

Silage fermentation is progressively restricted as the extent of pre-wilting increases (O'Kiely et_al., 1988). The magnitude of the improvement in silage nutritive value in response to a lactic acid bacteria inoculant could be related to the extent of the fermentation in the untreated silage. The objective of this experiment was to determine if the response in silage nutritive value to a Lactobacillus plantarum inoculant was similar at different levels of dry matter (DM) concentration.

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Epiphytic lactic acid bacteria succession during the pre-ensiling and ensiling periods of alfalfa and maize

Proceedings of the British Society of Animal Production (1972) ◽

10.1017/s0308229600022650 ◽

1992 ◽

Vol 1992 ◽

pp. 155-155

Author(s):

Chunjian Lin ◽

K. K. Bolsen ◽

B. E. Brent ◽

D.Y.C. Fung ◽

W. R. Aimutis

Keyword(s):

Zea Mays ◽

Lactic Acid ◽

North America ◽

Lactic Acid Bacteria ◽

Limited Information ◽

Maize Hybrids ◽

Silage Fermentation ◽

Whole Plant ◽

Three Stages ◽

Second Year

Epiphytic LAB, e.g., lactobacilli, lactococci, enterococci, pediococci, streptococci, and leuconostocs, play a major role in silage fermentation. Their numbers and populations have become a concern in predicting the adequacy of silage fermentation and in determining whether or not to apply a bacterial inoculant (Bolsen et al, 1989). Epiphytic LAB counts are usually low and variable on silage crops (Lin et al, 1991), and increases in the LAB counts usually occur coincident to the chopping process. Only limited information is available concerning the succession of epiphytic LAB species during the ensiling period of alfalfa (Medicago sativaL.) and maize (Zea mays L.), the two major silage crops in North America. The present studies investigated the epiphytic LAB succession during the pre-ensiling and ensiling periods for two cuttings of alfalfa, each harvested at three stages of maturity, and three whole-plant maize hybrids.A second-year stand of alfalfa was harvested at the 2nd and 4th cuttings and at the late-bud, 10% bloom, and 50% bloom stages of maturity within each cutting in 1989. Following mowing, the alfalfa was wilted in the windrow for 5 to 6 hours prior to precision chopping.

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