High frequency in vitro regeneration ofKigelia pinnata L. via organogenesis

High frequency in vitro regeneration for mass multiplication from immature male floral explants of Musa paradisica cv. Puttabale on MS supplemented with adenine sulfate (160 mg/l), tyrosine (100 mg/l), sucrose (40 g/l) and gelled with 0.8 g/l agar was attempted. For callus induction the combinations of 2, 4-D and BAP were tested at 1.0 - 10.0 mg/l and 0.5 - 5.0 mg/l, respectively. For shoot bud formation combinations of BAP and TDZ were also tested at 1.0 - 5.0 mg/l and 0.1 - 0.5 mg/l, respectively. Luxuriant proliferation and high frequency induction (97.0%) of callus was noticed from the accessory floral part of the explant at 7.0 mg/l 2, 4-D and 1.0 mg/l BAP, later it preceded towards the gynoecium. Interaction of BAP (2.0 5.0 mg/l) and TDZ (0.2 - 0.5 mg/l) would provoke high frequency shoot bud differentiation from the floral calli and a mean of 29.40 ± 6.10 shootlets per callus was obtained at 4 mg/l BAP and 0.4 mg/l TDZ. Rooting of the microshoots was achieved on MS containing 0.6 mg/l NAA and 0.2% activated charcoal. Key words: Musa pardisica, Puttabale, Regeneration, Male floral explants. D. O. I. 10.3329/ptcb.v21i2.10243 Plant Tissue Cult. & Biotech. 21(2): 199-205, 2011 (December)

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Mature Embryo-Based in vitro Regeneration of Indica Rice Cultivars for High Frequency Plantlets Production

Bangladesh Rice Journal ◽

10.3329/brj.v20i2.34132 ◽

2017 ◽

Vol 20 (2) ◽

pp. 81-87

Author(s):

HN Barman ◽

ME Hoque ◽

RK Roy ◽

PL Biswas ◽

MAI Khan ◽

...

Keyword(s):

Callus Induction ◽

High Frequency ◽

Indica Rice ◽

In Vitro Regeneration ◽

Mature Embryo ◽

Ms Medium ◽

Rice Varieties ◽

Growing Medium ◽

Regeneration Efficiency

The study was conducted at Biotechnology Division of Bangladesh Rice Research Institute (BRRI) to investigate the effects of plant growing medium and plant growth regulator (PGR) for the callus induction and high frequency plantlets regeneration of indica rice. Ten indica rice varieties viz. BR5, BR11, BRRI dhan28, BRRI dhan29, BRRI dhan33, BRRI dhan41, BRRI dhan47, BRRI dhan48, BRRI dhan49 and BRRI dhan50 were cultured on MS, N6 and LS media. The MS medium was found better for callus induction as compared to N6 and LS media. Among the tested varieties BRRI dhan48 induced the highest percent and best quality callus. Interaction effects of BRRI dhan48 to MS medium yielded 71.55% callus induction. The regeneration efficiency of BRRI dhan48 was tested on MS medium supplemented with different combination of NAA plus BAP and NAA plus kinetin. MS medium supplemented with 2.0 mg L-1 NAA and 2.0 mg L-1 Kn was found the best in respect of percent regenerated (76.67%) plantlet as well as for the growth of plantlets in vitro.Bangladesh Rice j. 2016, 20(2): 81-87

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High frequency in vitro regeneration of Lathyrus sativus L.

Biologia Plantarum ◽

10.1007/s10535-005-0066-x ◽

2005 ◽

Vol 49 (4) ◽

pp. 637-639 ◽

Cited By ~ 6

Author(s):

D. P. Barik ◽

U. Mohapatra ◽

P. K. Chand

Keyword(s):

High Frequency ◽

In Vitro Regeneration ◽

Lathyrus Sativus ◽

Lathyrus Sativus L

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EFFECTS OF DIFFERENT PHYSIOCHEMICAL FACTORS ON REGENERATION OF CYMBOPOGON POLYNEUROS STAPF VIA CALLUS CULTURE AND SUBSEQUENT CHROMOSOMAL STABILITY

Israel Journal of Plant Sciences ◽

10.1080/07929978.1999.10676773 ◽

1999 ◽

Vol 47 (3) ◽

pp. 195-198

Author(s):

Anath Bandhu Das

Keyword(s):

Callus Culture ◽

High Frequency ◽

Potassium Salt ◽

In Vitro Regeneration ◽

Basal Medium ◽

Naphthalene Acetic Acid ◽

Leaf Base ◽

Regenerated Plants ◽

The Individual

In vitro regeneration of Cymbopogon polyneuros Stapf was obtained through callus culture using leaf base, node, and root as explants. Callus was induced from different explants with 2–5 mg/1 α-naphthalene acetic acid (NAA) and 1–2 mg/1 kinetin in Murashige and Skoog's (MS) basal medium. High frequency shoots were noticed from leaf-base callus supplemented with 3.5 mg/1 6-benzylaminopurine (BA), L-arginine, adenine, and a low level of NAA (0.2 mg/1). About 80–85 shoot buds were obtained from ca. 200 mg of callus per culture. The individual shoots produced root in the presence of 0.5–3 mg/1 indole 3-butyric acid or its potassium salt. Regenerated plants were cytologically and phenotypically stable. Regenerants were transplanted into soil and subsequently transferred to the field.

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AN EFFICIENT IN VITRO REGENERATION METHOD TO PRODUCE ADVENTITIOUS PLANTS IN SWEETPOTATO

HortScience ◽

10.21273/hortsci.28.4.262b ◽

1993 ◽

Vol 28 (4) ◽

pp. 262B-262

Author(s):

C. S. Prakash ◽

R. Gosukonda ◽

A. Porobo Dessai ◽

E. Blay ◽

K. Dumenvo

Keyword(s):

Gene Transfer ◽

High Frequency ◽

In Vitro Regeneration ◽

Leaf Explants ◽

Zeatin Riboside ◽

Limiting Factor ◽

Ms Medium ◽

Petiole Explants ◽

Green House

Lack of suitable methods to develop adventitious plantlets in vitro is a limiting factor in producing transgenic sweetpotato plants through gene transfer. Studies were conducted to develop an in vitro high frequency regeneration protocol for sweetpotato that is rapid and consistent. When 27 genotypes of sweetpotato were screened, five were identified as highly regenerative (318846-3, PI 531143, Hi Dry, Rojoblanco and Beauregard). High frequency regeneration of shoots (in 60 to 80% explants) was observed within 30 days when leaf explants with intact petioles from the apical portions of the in vitro shoots were cultured on a MS medium with 2,4-D (0.2 mg/l) for three days and then transferred to a medium with zeatin riboside (ZR) (0.2 mg/l). However, thidiazuron (0.2 mg/l) had to be substituted for ZR to achieve regeneration of shoots from petiole (0.5 to 1 cm) explants (the most responsive organ for transformation by Agrobacterium). Petiole explants developed shoots efficiently (80-90%) and rapidly (10 to 21 d), but were specific to the genotype 318846-3. The resulting plantlets were vigorous and normal, and were transferred to the green house with little or no mortality.

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Optimization of Regeneration Protocol of Rice from Embryo Derived Callus

Progressive Agriculture ◽

10.3329/pa.v18i2.17461 ◽

2013 ◽

Vol 18 (2) ◽

pp. 25-33

Author(s):

N Kamal ◽

KM Nasuruddin ◽

MS Haque ◽

S Yasmin

Keyword(s):

High Frequency ◽

In Vitro Regeneration ◽

Basal Medium ◽

Ms Medium ◽

Rice Cultivars ◽

Regeneration Frequency ◽

Root Regeneration ◽

Maximum Root ◽

High Frequency Transformation

An efficient and reproducible protocol for in vitro regeneration is required to achieve high frequency transformation from transformed calli. We report here high frequency plant regeneration from mature embryonic calli of two BINA rice cultivars Binasail and Binadhan-4. Embryonic calllus initiated on MS basal medium supplemented with 2 mg/l 2, 4-D. Several media with different combinations of growth regulators were tried. Maximum shoot regeneration frequency (63.33%) was observed in Binadhan-4 on MS medium supplemented with 5 mg/l Kinetin + 0.5 mg/l NAA. Maximum root regeneration frequency (70.00%) was observed in Binadhan-4 on MS medium supplemented with 6 mg/l Kinetin + 0.5 mg/l NAA. Well developed plantlets were hardened and transferred to the glasshouse.DOI: http://dx.doi.org/10.3329/pa.v18i2.17461 Progress. Agric. 18(2): 25 - 33, 2007

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