Parasitism of frozen Halyomorpha halys eggs by Trissolcus japonicus: applications for rearing and experimentation

2019 ◽  
Vol 29 (5) ◽  
pp. 478-493 ◽  
Author(s):  
Hanna McIntosh ◽  
David M. Lowenstein ◽  
Nik G. Wiman ◽  
Jessica S. Wong ◽  
Jana C. Lee
Keyword(s):  
Halyomorpha Halys ◽  
Trissolcus Japonicus

scholarly journals The complete mitochondrial genome of Trissolcus japonicus (Hymenoptera: Scelionidae), the candidate for the biological control of Halyomorpha halys (Hemiptera: Pentatomidae)

2021 ◽  
Vol 6 (8) ◽  
pp. 2307-2309
Author(s):  
Francesco Nardi ◽  
Claudio Cucini ◽  
Elena Cardaioli ◽  
Francesco Paoli ◽  
Giuseppino Sabbatini Peverieri ◽  
...  
Keyword(s):  
Biological Control ◽  
Mitochondrial Genome ◽  
Complete Mitochondrial Genome ◽  
Halyomorpha Halys ◽  
Trissolcus Japonicus

scholarly journals Rearing Trissolcus japonicus and Trissolcus mitsukurii for Biological Control of Halyomorpha halys

Insects ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 787
Author(s):  
Giuseppino Sabbatini-Peverieri ◽  
Christine Dieckhoff ◽  
Lucrezia Giovannini ◽  
Leonardo Marianelli ◽  
Pio Federico Roversi ◽  
...  
Keyword(s):  
Biological Control ◽  
Control Method ◽  
Egg Parasitoids ◽  
Halyomorpha Halys ◽  
Progeny Production ◽  
Asian Origin ◽  
Frequent Use ◽  
Trissolcus Japonicus ◽  
Cumulative Curves

Halyomorpha halys is a severe agricultural pest of Asian origin that has invaded many countries throughout the world. Pesticides are currently the favored control methods, but as a consequence of their frequent use, often disrupt Integrated Pest Management. Biological control with egg parasitoids is seen as the most promising control method over the long-term. Knowledge of the reproductive biology under laboratory conditions of the most effective candidates (Trissolcus japonicus and Trissolcus mitsukurii) for optimizing production for field releases is strongly needed. Rearing of these egg parasitoids was tested by offering three different host supply regimes using new emerged females and aged, host-deprived females in different combinations. Results showed a mean progeny per female ranging from 80 to 85 specimens for T. japonicus and from 63 to 83 for T. mitsukurii. Sex ratios were strongly female biased in all combinations and emergence rates exceeded 94% overall. Cumulative curves showed that longer parasitization periods beyond 10–14 days (under the adopted rearing regimes) will not lead to a significantly increase in progeny production. However, ageing females accumulate eggs in their ovaries that can be quickly laid if a sufficient number of host eggs are supplied, thus optimizing host resources. Our data showed that offering H. halys egg masses to host-deprived female Trissolcus once a week for three weeks allowed its eggs to accumulate in the ovary, providing the greatest number of offspring within a three week span.

scholarly journals Floral Resources for Trissolcus japonicus, a Parasitoid of Halyomorpha halys

Insects ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 413
Author(s):  
Hanna R. McIntosh ◽  
Victoria P. Skillman ◽  
Gracie Galindo ◽  
Jana C. Lee
Keyword(s):  
Egg Parasitoid ◽  
Flowering Plant ◽  
Floral Resources ◽  
Halyomorpha Halys ◽  
Floral Nectar ◽  
Crimson Clover ◽  
Classical Biocontrol ◽  
Nectar Sources ◽  
Trissolcus Japonicus ◽  
The Impact

The egg parasitoid Trissolcus japonicus is the main candidate for classical biocontrol of the invasive agricultural pest Halyomorpha halys. The efficacy of classical biocontrol depends on the parasitoid’s survival and conservation in the agroecosystem. Most parasitoid species rely on floral nectar as a food source, thus identifying nectar sources for T. japonicus is critical. We evaluated the impact of eight flowering plant species on T. japonicus survival in the lab by exposing unfed wasps to flowers inside vials. We also measured the wasps’ nutrient levels to confirm feeding and energy storage using anthrone and vanillin assays adapted for T. japonicus. Buckwheat, cilantro, and dill provided the best nectar sources for T. japonicus by improving median survival by 15, 3.5, and 17.5 days compared to water. These three nectar sources increased wasps’ sugar levels, and cilantro and dill also increased glycogen levels. Sweet alyssum, marigold, crimson clover, yellow mustard, and phacelia did not improve wasp survival or nutrient reserves. Further research is needed to determine if these flowers maintain their benefits in the field and whether they will increase the parasitism rate of H. halys.

Behavioral responses of the egg parasitoid Trissolcus japonicus to volatiles from adults of its stink bug host, Halyomorpha halys

2017 ◽  
Vol 90 (4) ◽  
pp. 1097-1105 ◽  
Author(s):  
Yong-Zhi Zhong ◽  
Jin-Ping Zhang ◽  
Li-Li Ren ◽  
Rui Tang ◽  
Hai-Xia Zhan ◽  
...  
Keyword(s):  
Behavioral Responses ◽  
Egg Parasitoid ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Trissolcus Japonicus

scholarly journals An effective cold storage method for stockpiling Halyomorpha halys eggs for field surveys and laboratory rearing of Trissolcus japonicus

2020 ◽  
Author(s):  
Warren H. L. Wong ◽  
Matt A. Walz ◽  
Angela B. Oscienny ◽  
Jade L. Sherwood ◽  
Paul K. Abram
Keyword(s):  
Current Method ◽  
Field Studies ◽  
Egg Parasitoid ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Time Activity ◽  
Egg Masses ◽  
Fitness Consequences ◽  
Laboratory Colony ◽  
Trissolcus Japonicus

AbstractAn effective stockpiling method for egg masses of the invasive brown marmorated stink bug (Halyomorpha halys [Stål]; Hemiptera: Pentatomidae) would be useful for rearing and field studies of its egg parasitoid Trissolcus japonicus (Ashmead) (Hymenoptera: Scelionidae). The current method of stockpiling H. halys egg masses at sub-zero temperatures has lethal and sublethal fitness consequences for T. japonicus. We show that parasitoid emergence from egg masses refrigerated at 8°C for up to two months before parasitism is higher than from frozen egg masses and usually has minimal or no sublethal fitness effects (sex ratio, development time, activity, fecundity, longevity, and weight) on emerging T. japonicus. Only after two months of host egg refrigeration did the emergence of T. japonicus begin to decrease significantly (by 9.6% relative to untreated viable egg masses), whereas egg masses previously frozen at -80°C had a 58.8% reduction in parasitoid emergence after 14 days of storage. Refrigerated egg masses that were subsequently exposed to average field temperatures (warm: 22.9°C; cool: 13.2°C) were still suitable for T. japonicus parasitism after 7 days, while viable egg masses exposed to warm temperatures for 7 days before parasitism had 24.1% lower parasitoid emergence. Our results demonstrate that refrigeration at 8°C, while resulting in complete mortality of H. halys embryos after 10 days, are more suitable for T. japonicus parasitism than those stored at sub-zero temperatures. The quantity and quality of H. halys eggs that can be stockpiled with this method could facilitate T. japonicus laboratory colony maintenance, field monitoring, and releases.

scholarly journals Integrating Trissolcus japonicus (Ashmead, 1904) (Hymenoptera: Scelionidae) into Management Programs for Halyomorpha halys (Stål, 1855) (Hemiptera: Pentatomidae) in Apple Orchards: Impact of Insecticide Applications and Spray Patterns

Insects ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 833
Author(s):  
Dalton C. Ludwick ◽  
Jessica Patterson ◽  
Layne B. Leake ◽  
Lee Carper ◽  
Tracy C. Leskey
Keyword(s):  
The United States ◽  
Post Treatment ◽  
Egg Mass ◽  
Halyomorpha Halys ◽  
Apple Orchards ◽  
Specialty Crops ◽  
Egg Masses ◽  
Trissolcus Japonicus ◽  
Application Techniques ◽  
Pre Treatment

Halyomorpha halys (Stål, 1855) (Hemiptera: Pentatomidae) is an invasive species in the United States, where it has caused significant damage to specialty crops, including apples. While integrated pest management techniques have been developed for H. halys in apple, including spray application techniques, it is unknown how these techniques affect foraging, adventive Trissolcus japonicus (Ashmead, 1904) (Hymenoptera: Scelionidae), and its offspring. In this study, egg masses (unparasitized and 2 and 7 day parasitized pre-treatment) were placed in apple orchards in treated and untreated locations that received full block insecticide applications or reduced application techniques, including border row or alternate row middle applications. Bifenthrin, thiamethoxam + λ-cyhalothrin, clothianidin, and methomyl were evaluated. Egg masses were retrieved 24 h after spray applications. For 2 and 7 day parasitized pre-treatment, adult T. japonicus emergence was recorded from each egg mass. For unparasitized egg masses, T. japonicus females were given 24 h to forage and oviposit on post-treatment egg masses with female survivorship, and adult emergence from egg masses was recorded. Female survivorship was significantly lower on post-treatment egg masses retrieved from areas receiving bifenthrin applications. Emergence from post-treatment egg masses was affected by thiamethoxam + λ-cyhalothrin, bifenthrin, and methomyl in some treated areas, whereas less impact was observed on 2 and 7 day pre-treatment parasitized egg masses in general. These data provide further insights into H. halys management and the potential impact of T. japonicus in sprayed orchard agroecosystems.

scholarly journals Influence of Holding Conditions and Storage Duration of Halyomorpha halys (Hemiptera: Pentatomidae) Eggs on Adventive and Quarantine Populations of Trissolcus japonicus (Hymenoptera: Scelionidae) Behavior and Parasitism Success

2021 ◽  
Author(s):  
Dalton C Ludwick ◽  
Layne B Leake ◽  
William R Morrison ◽  
Jesús R Lara ◽  
Mark S Hoddle ◽  
...  
Keyword(s):  
United States ◽  
Classical Biological Control ◽  
The United States ◽  
Invasive Pest ◽  
Egg Mass ◽  
Halyomorpha Halys ◽  
Storage Duration ◽  
Egg Masses ◽  
Trissolcus Japonicus ◽  
And Storage

Abstract Halyomorpha halys (Stål) is an invasive pest in the United States and other countries. In its native range, H. halys eggs are parasitized by a co-evolved parasitoid, Trissolcus japonicus (Ashmead). In the United States, T. japonicus, a classical biological control candidate, is being redistributed in many states where adventive populations exist. To establish if H. halys egg holding conditions affect T. japonicus foraging behavior or successful parasitism, naïve, female parasitoids from an adventive population were allowed to forage in laboratory bioassay arenas with either fresh or frozen (−20 or −80°C) egg masses, the latter held for five durations ranging from 1 h to 112 d. Parasitoid movements were recorded for 1 h. Thereafter, parasitoids were transferred with the same egg mass for 23 h. Additionally, female parasitoids from a quarantine colony were exposed to: 1) pairs of fresh egg masses and egg masses frozen at −40°C (>24 h) or 2) a single fresh egg mass or egg mass frozen at −40°C (<1 h). All exposed egg masses were held to assess progeny emergence. In the foraging bioassay, holding temperature and storage duration appeared to influence host-finding and host quality. Egg masses held at −80°C and fresh egg masses resulted in significantly greater levels of parasitism and progeny emergence compared with eggs held at −20°C. No differences were recorded between egg masses held at −40°C for ≤1 h and fresh egg masses. These results will help refine methods for preparation of egg masses for sentinel monitoring and parasitoid mass rearing protocols.

Sign in / Sign up

Export Citation Format

Share Document