scholarly journals Cytokine profile of Ehrlich ascites tumor treated withBothrops jararacavenom

2002 ◽  
Vol 11 (4) ◽  
pp. 197-201 ◽  
Author(s):  
Reinaldo J. da Silva ◽  
Márcia G. da Silva ◽  
Lízia C. Vilela ◽  
Denise Fecchio

We previously demonstrated thatBothrops jararacavenom (BjV) has an antitumor effect on Ehrlich ascites tumor (EAT) cells and induces an increase of polymorphonuclear leukocytes in early stages of tumor growth. It has been reported that this venom presents an important inflammatory effect when inoculated in animal models and in human snakebites, and that cytokine levels have been detected in these cases. To evaluate whether the cytokines can be involved with the suppression of the tumoral growth, we evaluate the cytokine profile in the peritoneal cavity of mice inoculated with EAT cells and treated withBjV. Swiss mice were inoculated with EAT cells by the intraperitoneal route and treated withBjV venom (0.4 mg/kg, intraperitoneally), on the 1st, 4th, 7th, 10th, and 13th day. Mice were evaluated for cytokine levels on the 2nd, 5th, 8th, 11th and 14th day. Analysis was performed using an enzyme-linked immunosorbent assay for interleukin (IL)-1α, IL-2, IL-4, IL-6, IL-10, IL-13, and tumor necrosis factor-α (TNF-α) levels in the peritoneal washing supernatant. Results were analyzed statistically by the Kruskal-Wallis and Dunn's tests at the 5% level of significance. We observed that EAT implantation induces IL-6 production on the 11th and 14th days of tumor growth, IL-10 on the 11th day and TNF-α on the 14th day. The treatment withBjV suppresses production of these cytokines. In addition, IL-13 was produced by animals that were inoculated only with venom on the 11th and 14th days, and by the group inoculated with EAT cells and treated with venom on the 2nd and 14th days. Furthermore, we suggest that the IL-6 detected in the present study is produced by the EAT cells and the suppression of its production could be associated with the antitumor effect ofBjV.

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Luciana Boffoni Gentile ◽  
Nicolle Queiroz-Hazarbassanov ◽  
Cristina de Oliveira Massoco ◽  
Denise Fecchio

The aim of the present study was to investigate the influence of a nonselective COX1/COX2 inhibitor (indomethacin) on tumor growth of Ehrlich Ascites Tumor (EAT) in mice, using as parameters the tumor growth and cytokine profile. Mice were inoculated with EAT cells and treated with indomethacin. After 1, 3, 6, 10, and 13 days the animals were evaluated for the secretion of TNFα, IL-1α, IL-2, IL-4, IL-6, IL-10, and IL-13 and PGE2level in peritoneal cavity. The results have shown that EAT induces PGE2production and increases tumor cells number from the 10th day. The cytokine profile showed EAT induces production of IL-6 from 10th day and of IL-2 on 13th day; the other studied cytokines were not affected in a significant way. The indomethacin treatment of EAT-bearing mice inhibited the tumor growth and PGE2synthesis from the 10th day. In addition, the treatment of EAT-bearing mice with indomethacin has stimulated the IL-13 production and has significantly inhibited IL-6 in the 13th day of tumor growth. Taken together, the results have demonstrated that EAT growth is modulated by PGE2and the inhibition of the tumor growth could be partly related to suppression of IL-6 and induction of IL-13.


1960 ◽  
Vol 80 (9) ◽  
pp. 1291-1292
Author(s):  
Mihoko Abe ◽  
Hideo Hirokawa ◽  
Yukito Masamune ◽  
Den'ichi Mizuno

1963 ◽  
Vol 18 (7) ◽  
pp. 523-525 ◽  
Author(s):  
Rudolf K. Zahn ◽  
Ekkehard Tiesler

1. Deoxyribonuclease activity has been determined by viscosimetry in the serum of the Ehrlich Ascites in the mouse to be equivalent to 2,3 ± 0,7·10-6 g/ml of crystalline beef pancreas DNase.2. Injections of heterologous deoxyribonucleic acid into the Ehrlich Ascites tumor under specified conditions does not alter tumor development.3. The same DNA however becomes strongly inhibitory for tumor growth after UV irradiation.4. Possible implications are discussed.


1958 ◽  
Vol 78 (3) ◽  
pp. 242-244 ◽  
Author(s):  
Tomoyoshi Komai ◽  
Hiroko Higurashi ◽  
Mihoko Abe ◽  
Den-ichi Mizuno

2007 ◽  
Vol 15 (04) ◽  
pp. 453-471 ◽  
Author(s):  
MAREK BODNAR ◽  
URSZULA FORYŚ

In this paper, we compare three types of dynamical systems used to describe tumor growth. These systems are defined as solutions to three delay differential equations: the logistic, the Gompertz and the Greenspan types. We present analysis of these systems and compare with experimental data for Ehrlich Ascites tumor in mice.


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