scholarly journals An immunoassay for the measurement of (1→3)-β-D-glucans in the indoor environment

1997 ◽  
Vol 6 (4) ◽  
pp. 257-262 ◽  
Author(s):  
Jeroen Douwes ◽  
Gert Doekes ◽  
Roy Montijn ◽  
Dick Heederik ◽  
Bert Brunekreef
Keyword(s):  
Enzyme Immunoassay ◽  
Exposure Assessment ◽  
Calibration Curve ◽  
Bovine Serum ◽  
Indoor Environment ◽  
Weight Basis ◽  
Inhibition Curve

An inhibition enzyme immunoassay was developed for quantitation of (1→3)-β-D-glucans in the indoor environment. Immunospecific rabbit antibodies were produced by immunization with bovine serum albuminconjugated laminarin.The laminarin calibration curve ranged from 40 to 3000 ng/ml.Another (1→3)-β-D-glucan (curdlan) showed a similar inhibition curve, but was less reactive on a weight basis. Pustulan, presumed to be (1→3)-β-D-glucan, also showed immunoreactivity in the assay. Control experiments indicated that this was due to (1→3)-β-D-glucan structures. Other non-(1→3)-β-D-glucan polysaccharides did not react. (1→3)-β-Dglucan was detectable in dust from a variety of occupational and environmental settings. We conclude that the new assay offers a useful method for indoor (1→3)-β-Dglucan exposure assessment.

scholarly journals Developing an Automated Enzyme Immunoassay for High-Throughput Screening of Bovine Serum Antibodies to Neospora Caninum

2003 ◽  
Vol 8 (1) ◽  
pp. 46-50
Author(s):  
John T. Y. Wu ◽  
Sally Dreger ◽  
Eva Y. W. Chow ◽  
Evelyn E. Bowlby ◽  
Lester S. Y. Wong
Keyword(s):  
Enzyme Immunoassay ◽  
High Throughput ◽  
Bovine Serum ◽  
High Throughput Screening ◽  
Neospora Caninum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Predictive Values ◽  
Automated Assay ◽  
Robotic Workstation ◽  
Elisa Data

An enzyme-linked immunosorbent assay (ELISA) for Neospora caninum antibodies was automated with a robotic workstation, the Beckman Coulter Biomek 2000, to screen 200 bovine sera. Comparing these results with manually run ELISA data, a 95.92% agreement (K = 0.9592) between the two assays was obtained. The automated assay was specific and sensitive with excellent positive and negative predictive values. The results were repeatable and reproducible. The automation flexibility was high and the operation complexity was minimal. High-throughput screening (HTS) for bovine antibodies to Neospora caninum was achieved. The assay was developed according to the internationally recognized ISO17025 standard requirements.

Analysis of global commonly-used phthalates and non-dietary exposure assessment in indoor environment

2020 ◽  
Vol 177 ◽  
pp. 106853 ◽  
Author(s):  
Subei Bu ◽  
Yanling Wang ◽  
Haiyan Wang ◽  
Fang Wang ◽  
Yufei Tan
Keyword(s):  
Exposure Assessment ◽  
Indoor Environment ◽  
Dietary Exposure

Indirect Competitive Enzyme Immunoassay for Tetrodotoxin Using a Biotin-Avidin System

1992 ◽  
Vol 75 (5) ◽  
pp. 883-886 ◽  
Author(s):  
Kendo Matsumura ◽  
Sadako Fukiya
Keyword(s):  
Bovine Serum Albumin ◽  
Enzyme Immunoassay ◽  
Immunoglobulin G ◽  
Bovine Serum ◽  
Keyhole Limpet Hemocyanin ◽  
Microtiter Plate ◽  
Mouse Bioassay ◽  
Detection Level ◽  
Standard Curve ◽  
Peroxidase Conjugate

Abstract An indirect competitive enzyme immunoassay was developed to determine tetrodotoxin (TTX). Antiserum against TTX was demonstrated in rabbits immunized with TTX-keyhole limpet hemocyanin conjugate. In this assay, TTX-bovine serum albumin was coated on the microtiter plate, which was incubated with standard TTX and biotinylated immunoglobulin G from antiserum. The amount of antibody bound on the surface of wells was determined by the reaction of avidin-peroxidase conjugate with its substrate. The minimal detection level of TTX ranged from 5 to 25 pg/assay. The standard curve was linear between 0.25 and 50 ng/assay. A good correlation (r= 0.974) for TTX was also obtained between this indirect competitive enzyme immunoassay and the mouse bioassay.

Exposure Assessment Analysis of a Heated Breathing Manikin With Rotation in a Displacement Ventilated Room by Numerical Methods

2014 ◽  
Author(s):  
S. A. Keshavarz ◽  
M. Salmanzadeh ◽  
G. Ahmadi
Keyword(s):  
Numerical Methods ◽  
Exposure Assessment ◽  
Indoor Environment ◽  
Human Motion ◽  
The Body ◽  
Thermal Plume ◽  
Indoor Environments ◽  
Human Respiration ◽  
Airflow Field ◽  
Simulation Results

It is well known that the airflow is instrumental in the transmission of airborne infectious diseases in indoor environments. The airflow pattern in indoor environment is affected by the ventilation airflow, thermal plume around human bodies, human respiration, human motion and other activities. In this study, the CFD approach was used to simulate airflow field and particle transport in a room to provide exposure assessment for a heated breathing manikin with and without rotational motion. The simulation results indicated that the rotation of the manikin significantly impacts the thermal plume of the body and the associated transport of particulates.

scholarly journals A Critical Evaluation of a New Fluorescence Immunoassay System for the Measurement of Serum Phenytoin Concentrations

1983 ◽  
Vol 20 (5) ◽  
pp. 274-279 ◽  
Author(s):  
S J Davis ◽  
V Marks
Keyword(s):  
Liquid Chromatography ◽  
Enzyme Immunoassay ◽  
Temperature Control ◽  
Calibration Curve ◽  
Incubation Time ◽  
Incubation Temperature ◽  
Critical Evaluation ◽  
Fluorescence Immunoassay ◽  
Significant Interference ◽  
Single Calibration

Results are presented which show that the Ames TDA™ phenytoin kit may be used with a shortened incubation time (5 minutes) and that, using a Fluorostat™ instrument, a single calibration curve can be stored and used effectively over a three-week period. The importance of temperature control when using a single calibration curve is emphasised. An unexplained finding was that higher results were obtained for phenytoin concentrations up to about 15 mg/1 with increasing incubation temperature. There was acceptable precision of measurement at all levels of phenytoin concentration, and there was no significant interference by haemoglobin, bilirubin, or lipids. The TDA results correlated well with those obtained using liquid chromatography ( y=0·39 + 0·98 x, r=0·98, where y=TDA and x=HPLC) and enzyme immunoassay ( y=0·08 + 0·93 x, r=0·96, where y=TDA and x=EIA).

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