Comparative kinetic study on biodecolorization of synthetic dyes by Bjerkandera adusta SM46 in alginate beads-packed bioreactor system and shaking culture under saline-alkaline stress

2021 ◽  
pp. 1-12
Author(s):  
Ade Andriani ◽  
Dede Heri Yuli Yanto
Keyword(s):  
Kinetic Study ◽  
Alginate Beads ◽  
Alkaline Stress ◽  
Synthetic Dyes ◽  
Bjerkandera Adusta ◽  
Bioreactor System

scholarly journals The Enzymatic Decolorization of Textile Dyes by the Immobilized Polyphenol Oxidase from Quince Leaves

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Gulnur Arabaci ◽  
Ayse Usluoglu
Keyword(s):  
Polyphenol Oxidase ◽  
Calcium Alginate ◽  
Enzyme Stability ◽  
Industrial Effluent ◽  
Alginate Beads ◽  
Textile Dyes ◽  
Kinetic Properties ◽  
Synthetic Dyes ◽  
Cydonia Oblonga ◽  
Calcium Alginate Beads

Water pollution due to release of industrial wastewater has already become a serious problem in almost every industry using dyes to color its products. In this work, polyphenol oxidase enzyme from quince (Cydonia Oblonga) leaves immobilized on calcium alginate beads was used for the successful and effective decolorization of textile industrial effluent. Polyphenol oxidase (PPO) enzyme was extracted from quince (Cydonia Oblonga) leaves and immobilized on calcium alginate beads. The kinetic properties of free and immobilized PPO were determined. Quince leaf PPO enzyme stability was increased after immobilization. The immobilized and free enzymes were employed for the decolorization of textile dyes. The dye solutions were prepared in the concentration of 100 mg/L in distilled water and incubated with free and immobilized quince (Cydonia Oblonga) leaf PPO for one hour. The percent decolorization was calculated by taking untreated dye solution. Immobilized PPO was significantly more effective in decolorizing the dyes as compared to free enzyme. Our results showed that the immobilized quince leaf PPO enzyme could be efficiently used for the removal of synthetic dyes from industrial effluents.

scholarly journals Development of a bioreactor system for the remediation of endosulphan

2018 ◽  
Vol 2 (1) ◽  
pp. 1-8 ◽  
Author(s):  
K. Jesitha ◽  
P. S. Harikumar
Keyword(s):  
Detection Limit ◽  
Calcium Alginate ◽  
Packed Bed ◽  
Alginate Beads ◽  
Soil Samples ◽  
Packed Bed Reactor ◽  
Contaminated Water ◽  
Initial Concentration ◽  
Bioreactor System ◽  
Ca Alginate

Abstract A bioreactor system that consisted of Pseudomonas fluorescens cells immobilised in calcium-alginate beads was utilised to remediate endosulphan contaminated water and soil. A packed bed reactor system was designed for the bio-degradation of endosulphan in artificially spiked water samples (initial concentration of endosulphan: 350 µg/L). Reactor studies with cell-immobilised Ca-alginate beads were conducted after checking their efficiency through batch and column degradation studies. The results showed that the concentration of toxic isomers of endosulphan (endosulphan alpha and endosulphan beta) was below the limit in the bioreactor during the 7th day of the experiment. Experiments conducted with contaminated soil samples (initial concentration of endosulphan: 1,000 μg/kg) indicated that the toxic isomers of endosulphan degraded to below the detection limit within 10 days and monitoring of endosulphan residues on the 14th day revealed that almost complete degradation of metabolites of endosulphan had occurred. The bioreactor system designed can be scaled up for remediation of endosulphan in contaminated areas.

Biodegradation and kinetic study of benzene in bioreactor packed with PUF and alginate beads and immobilized with Bacillus sp. M3

2017 ◽  
Vol 242 ◽  
pp. 92-100 ◽  
Author(s):  
M.K. Kureel ◽  
S.R. Geed ◽  
B.S. Giri ◽  
B.N. Rai ◽  
R.S. Singh
Keyword(s):  
Kinetic Study ◽  
Alginate Beads ◽  
Bacillus Sp

Kinetic Study of a Glucose Tolerant β-Glucosidase from Aspergillus fumigatus ABK9 Entrapped into Alginate Beads

2014 ◽  
Vol 6 (1) ◽  
pp. 53-61 ◽  
Author(s):  
Arpan Das ◽  
Tanmay Paul ◽  
Priyanka Ghosh ◽  
Suman Kumar Halder ◽  
Pradeep Kumar Das Mohapatra ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Kinetic Study ◽  
Alginate Beads ◽  
Glucose Tolerant

Impact of ciprofloxacin, carbamazepine and ibuprofen on a membrane bioreactor system: Kinetic study and biodegradation capacity

2017 ◽  
Vol 92 (12) ◽  
pp. 2944-2951 ◽  
Author(s):  
Gustavo Calero-Díaz ◽  
Antonio Monteoliva-García ◽  
Juan Carlos Leyva-Díaz ◽  
Cristina López-López ◽  
Jaime Martín-Pascual ◽  
...  
Keyword(s):  
Kinetic Study ◽  
Membrane Bioreactor ◽  
Bioreactor System

Kinetic Study of the Adsorption of Synthetic Dyes on Graphene Surfaces

2014 ◽  
Vol 9 (4) ◽  
pp. 278-367 ◽  
Author(s):  
Ramin Shahryari Ghoshekandi ◽  
Hamidreza Sadegh
Keyword(s):  
Kinetic Study ◽  
Synthetic Dyes

Alginate Microbeads as Cell Support for Cartilage Tissue Engineering: Bioreactor Studies

2007 ◽  
Vol 555 ◽  
pp. 417-422 ◽  
Author(s):  
B. Obradović ◽  
A. Osmokrović ◽  
B. Bugarski ◽  
D. Bugarski ◽  
G. Vunjak-Novaković
Keyword(s):  
Tissue Engineering ◽  
Mass Transport ◽  
Immobilized Cells ◽  
Cartilage Tissue Engineering ◽  
Packed Bed ◽  
Alginate Beads ◽  
Cartilage Tissue ◽  
Extracellular Matrix Components ◽  
Bioreactor System

Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 μm) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering.

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