scholarly journals Development of a bioreactor system for the remediation of endosulphan

2018 ◽  
Vol 2 (1) ◽  
pp. 1-8 ◽  
Author(s):  
K. Jesitha ◽  
P. S. Harikumar
Keyword(s):  
Detection Limit ◽  
Calcium Alginate ◽  
Packed Bed ◽  
Alginate Beads ◽  
Soil Samples ◽  
Packed Bed Reactor ◽  
Contaminated Water ◽  
Initial Concentration ◽  
Bioreactor System ◽  
Ca Alginate

Abstract A bioreactor system that consisted of Pseudomonas fluorescens cells immobilised in calcium-alginate beads was utilised to remediate endosulphan contaminated water and soil. A packed bed reactor system was designed for the bio-degradation of endosulphan in artificially spiked water samples (initial concentration of endosulphan: 350 µg/L). Reactor studies with cell-immobilised Ca-alginate beads were conducted after checking their efficiency through batch and column degradation studies. The results showed that the concentration of toxic isomers of endosulphan (endosulphan alpha and endosulphan beta) was below the limit in the bioreactor during the 7th day of the experiment. Experiments conducted with contaminated soil samples (initial concentration of endosulphan: 1,000 μg/kg) indicated that the toxic isomers of endosulphan degraded to below the detection limit within 10 days and monitoring of endosulphan residues on the 14th day revealed that almost complete degradation of metabolites of endosulphan had occurred. The bioreactor system designed can be scaled up for remediation of endosulphan in contaminated areas.

scholarly journals Immobilization of Lycinibacillus fusiformis B26 cells in different matrices for use in turquoise blue HFG decolourization

2016 ◽  
Vol 42 (2) ◽  
pp. 92-99 ◽  
Author(s):  
Nazime Mercan Dogan ◽  
Tugba Sensoy ◽  
Gulumser Acar Doganli ◽  
Naime Nur Bozbeyoglu ◽  
Dicle Arar ◽  
...  
Keyword(s):  
Calcium Alginate ◽  
Immobilized Cells ◽  
Alginate Beads ◽  
Optimum Conditions ◽  
Colour Removal ◽  
Operational Conditions ◽  
Cell Pellet ◽  
Cell Concentrations ◽  
Alginate Matrices ◽  
Ca Alginate

Abstract The decolourization of Turquoise Blue HFG by immobilized cells of Lysinibacillus fusiformis B26 was investigated. Cells of L. fusiformis B26 were immobilized by entrapment in agar and calcium alginate matrices and attached in pumice particles. The effects of operational conditions (e.g., agar concentrations, cell concentrations, temperature, and inoculum amount) on microbial decolourization by immobilized cells were investigated. The results revealed that alginate was proven to be the best as exhibiting maximum decolourization (69.62%), followed by agar (55.55%) at 40°C. Pumice particles were the poorest. Optimum conditions for agar matrix were found: concentration was 3%, cell amount was 0.5 g and temperature was 40°C (55.55%). Ca-alginate beads were loaded with 0.5, 1.0 and 2.0 g of wet cell pellets and the highest colour removal activity was observed with 2.0 g of cell pellet at 40°C for alginate beads. Also, 0.5 and 1.0 g of pumice particles that were loaded with 0.25 and 0.5 g of cell pellets respectively were used and the results were found very similar to each other.

Influence of the addition of sulphate and ferric ions in a methanogenic anaerobic packed-bed reactor treating gasoline-contaminated water

2006 ◽  
Vol 54 (2) ◽  
pp. 135-141 ◽  
Author(s):  
B.S. Fernandes ◽  
F.A. Chinalia ◽  
A. Sarti ◽  
A.J. Silva ◽  
E. Foresti ◽  
...  
Keyword(s):  
Aromatic Compounds ◽  
Storage Tank ◽  
Packed Bed ◽  
Methanosarcina Barkeri ◽  
Packed Bed Reactor ◽  
Contaminated Water ◽  
Ferric Ions ◽  
Degradation Rates ◽  
Methanogenic Condition ◽  
Benzene Toluene

Benzene, toluene and xylene (BTX) are relatively soluble aromatic compounds of gasoline. Gasoline storage tank leakages generally lead to an extensive contamination of groundwater. In the natural environment for instance, these compounds might be biodegraded under a variety of reducing potentials. The objective of this work was to examine the influence of the addition of sulphate and Fe(OH)3 in a methanogenic horizontal-flow anaerobic immobilized-biomass reactor treating gasoline-contaminated water. Three different conditions were evaluated: methanogenic, sulphidogenic and sulphidogenic with the addition of ferric ions. Methanogenic condition showed the higher BTX degradation rates and the addition of sulphate negatively affected BTX removal rates with the production of H2S. However, the addition of ferric ions resulted in the precipitation of sulphur, improving BTX degradation by the consortium. Metanosphaera sp., Methanosarcina barkeri and Methanosaeta concilii were identified in the consortium by means of 16S and directly related to the addition of ferric ions.

Immobilization of pectinase from Aspergillus aculeatus in alginate beads and clarification of apple and umbu juices in a packed bed reactor

2018 ◽  
Vol 109 ◽  
pp. 9-18 ◽  
Author(s):  
Rodrigo Lira de Oliveira ◽  
Jônatas Lopes Dias ◽  
Osmar Soares da Silva ◽  
Tatiana Souza Porto
Keyword(s):  
Packed Bed ◽  
Alginate Beads ◽  
Packed Bed Reactor ◽  
Aspergillus Aculeatus

Immobilized Whole Cells as Effective Catalysts for Chiral Alcohol Production

2009 ◽  
Vol 62 (9) ◽  
pp. 1034 ◽  
Author(s):  
Jeck Fei Ng ◽  
Stephan Jaenicke
Keyword(s):  
Flow Reactor ◽  
Batch Reactor ◽  
Enantiomeric Excess ◽  
Lactobacillus Brevis ◽  
Packed Bed ◽  
Alginate Beads ◽  
Packed Bed Reactor ◽  
Process Conditions ◽  
Alcohol Production ◽  
Whole Cells

Recombinant Escherichia coli overexpressing the gene LbADH, which encodes for an alcohol dehydrogenase from Lactobacillus brevis, was successfully transformed and cultured. The cells are able to catalyze the reduction of pro-chiral ketones, e.g. ethyl acetoacetate into R-(–)ethyl hydroxybutyrate (EHB) with high conversion and enantiomeric excess >99%. Immobilizing the whole cells in alginate beads leads to a catalyst with improved stability and ease of handling while maintaining the high activity of the free cells. The whole-cell catalyst was tested in a stirred batch reactor (CSTR) and in a continuously operated packed-bed reactor. An Mg2+ concentration of 2 mM was crucial for maintaining the activity of the biocatalyst. After a partial optimization of the process conditions, a productivity of 1.4 gEHB gwcw–1 h–1 could be maintained in a continuous flow reactor over a prolonged period of time.

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