The downregulation of NADPH oxidase Nox4 during hypoxia in hemangioendothelioma cells: a possible role of p22phox on Nox4 protein stability

2022 ◽  
pp. 1-9
Author(s):  
Kei Miyano ◽  
Shuichiro Okamoto ◽  
Akira Yamauchi ◽  
Chikage Kawai ◽  
Mizuho Kajikawa ◽  
...  
Immunology ◽  
2010 ◽  
Vol 131 (3) ◽  
pp. 331-339 ◽  
Author(s):  
Takashi Miyoshi ◽  
Kouhei Yamashita ◽  
Toshiyuki Arai ◽  
Kokichi Yamamoto ◽  
Kiyomi Mizugishi ◽  
...  

2009 ◽  
Vol 5 (1) ◽  
pp. 83-92 ◽  
Author(s):  
Francesco Violi ◽  
Stefania Basili ◽  
Carmen Nigro ◽  
Pasquale Pignatelli
Keyword(s):  

Hypertension ◽  
2000 ◽  
Vol 36 (suppl_1) ◽  
pp. 688-688
Author(s):  
Toshihiro Ichiki ◽  
Kotaro Takeda ◽  
Akira Takeshita

58 Recent studies suggest a crucial role of reactive oxygen species (ROS) for the signaling of Angiotensin II (Ang II) through type 1 Ang II receptor (AT1-R). However, the role of ROS in the regulation of AT1-R expression has not been explored. In this study, we examined the effect of an antioxidant on the homologous downregulation of AT1-R by Ang II. Ang II (10 -6 mol/L) decreased AT1-R mRNA with a peak suppression at 6 hours of stimulation in rat aortic vascular smooth muscle cells (VSMC). Ang II dose-dependently (10 -8 -10 -6 ) suppressed AT1-R mRNA at 6 hours of stimulation. Preincubation of VSMC with N-acetylcysteine (NAC), a potent antioxidant, almost completely inhibited the Ang II-induced downregulation of AT1-R mRNA. The effect of NAC was due to stabilization of the AT1-R mRNA that was destabilized by Ang II. Ang II did not affect the promoter activity of AT1-R gene. Diphenylene iodonium (DPI), an inhibitor of NADH/NADPH oxidase failed to inhibit the Ang II-induced AT1-R mRNA downregulation. The Ang II-induced AT1-R mRNA downregulation was also blocked by PD98059, an extracellular signal-regulated protein kinase (ERK) kinase inhibitor. Ang II-induced ERK activation was inhibited by NAC as well as PD98059 whereas DPI did not inhibit it. To confirm the role of ROS in the regulation of AT1-R mRNA expression, VSMC were stimulated with H 2 O 2 . H 2 O 2 suppressed the AT1-R mRNA expression and activated ERK. These results suggest that production of ROS and activation of ERK are critical for downregulation of AT1-R mRNA. The differential effect of NAC and DPI on the downregulation of AT1-R mRNA may suggest the presence of other sources than NADH/NADPH oxidase pathway for ROS in Ang II signaling. Generation of ROS through stimulation of AT1-R not only mediates signaling of Ang II but may play a crucial role in the adaptation process of AT1-R to the sustained stimulation of Ang II.


2019 ◽  
Vol 31 (1) ◽  
pp. 39-58 ◽  
Author(s):  
Mark D. Stevenson ◽  
Chandrika Canugovi ◽  
Aleksandr E. Vendrov ◽  
Takayuki Hayami ◽  
Dawn E. Bowles ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document