EFFECTS OF INCREASED SALINITY ON GLUCOSE AND MALTOSE COMPOSITION OF BIOMPHALARIA GLABRATA SNAILS AS DETERMINED BY HIGH PERFORMANCE THIN-LAYER CHROMATOGRAPHY-DENSITOMETRY

2012 ◽  
Vol 35 (10) ◽  
pp. 1452-1458 ◽  
Author(s):  
Nevena Popovic ◽  
Bernard Fried ◽  
Joseph Sherma
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biomphalaria Glabrata ◽  
Layer Chromatography

Effects of Echinostoma caproni miracidia dose on the amino acid contents of Biomphalaria glabrata as determined by high-performance thin-layer chromatography

2016 ◽  
Vol 61 (1) ◽  
Author(s):  
Mai Nguyen ◽  
Bernard Fried ◽  
Joseph Sherma
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biomphalaria Glabrata ◽  
High Dose ◽  
Echinostoma Caproni ◽  
Amino Acid Contents ◽  
Layer Chromatography

AbstractThe effects of 5, 20, and 40 miracidia dose exposures of Echinostoma caproni on the amino acid contents of Biomphalaria glabrata were studied using high performance thin-layer chromatography-densitometry. Amino acids were identified and quantified in whole bodies of exposed snails and in the uninfected matched controls at 2 and 4 weeks post-exposure. Using cellulose layers with the mobile phase 2-butanol-pyridine-glacial acetic acid-deionized water (39:34:10:26) and ninhydrin detection reagent [2% ninhydrin in acetone-n-butanol (1:1)], five amino acids were identified, i.e., leucine/isoleucine, valine, alanine, glycine, and ornithine, by hRF value comparison and color differentiation. Quantitatively, there was a marked elevation in the amounts of four of these five amino acids (isoleucine/leucine, valine, alanine, and ornithine) across dose levels at 4 weeks post-infection (P<0.05). Elevation of the amino acid content in the high dose snail group suggested that some changes occurred in the amino acid metabolism of the snails in that group as a function of miracidia dose.

Effects of Schistosoma mansoni infection on lutein and β-carotene concentrations in Biomphalaria glabrata snails as determined by quantitative high performance reversed phase thin-layer chromatography

2013 ◽  
Vol 58 (3) ◽  
Author(s):  
Nicole Dieterich ◽  
Bernard Fried ◽  
Joseph Sherma
Keyword(s):  
Thin Layer ◽  
Schistosoma Mansoni ◽  
Thin Layer Chromatography ◽  
Post Infection ◽  
High Performance ◽  
Biomphalaria Glabrata ◽  
Reversed Phase ◽  
Whole Body ◽  
Layer Chromatography ◽  
Β Carotene

AbstractHigh performance thin-layer chromatography was used to determine the concentration of β-carotene and lutein in the whole body and digestive gland-gonad complex (DGG) of uninfected Biomphalaria glabrata snails and those infected with Schistosoma mansoni for 6 and 8 weeks. Pigments were extracted from the snails using acetone and separated on EMD Millipore reversed phase C-18 plates with concentration zone using petroleum ether-acetonitrile-methanol (1:1:2) mobile phase. After development, two yellow pigment zones, lutein and β-carotene, were identified with respective R f values of 0.55 and 0.13 and then quantified by densitometry. Statistical analysis of the weight percentages of each pigment showed a significant decrease (P < 0.05) in the concentration of β-carotene in the DGGs of infected B. glabrata at 6 and 8 weeks post-infection compared to the uninfected snails. No significant differences were seen in the concentrations of β-carotene in the whole body of the uninfected versus infected snail samples. Changes in the lutein concentration of the infected DGG and whole snail bodies were insignificant compared to the uninfected controls. In conclusion, larval S. mansoni infection caused a significant decrease in the β-carotene concentration of the DGG at 6 and 8 weeks post infection.

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