In vitro mass propagation and conservation of Nilgirianthus ciliatus through nodal explants: A globally endangered, high trade medicinal plant of Western Ghats

2016 ◽  
Vol 151 (2) ◽  
pp. 204-211 ◽  
Author(s):  
R. Rameshkumar ◽  
M. J. V. Largia ◽  
L. Satish ◽  
J. Shilpha ◽  
M. Ramesh
Keyword(s):  
Medicinal Plant ◽  
Western Ghats ◽  
Nodal Explants ◽  
Mass Propagation

scholarly journals Mass Propagation of Feronia limonia L. through Tissue Culture

1970 ◽  
Vol 45 (1) ◽  
pp. 75-78 ◽  
Author(s):  
Shahina Islam ◽  
Mosfequa Zahan ◽  
Shahina Akter ◽  
Tanjina Akhtar Banu ◽  
Ahashan Habib ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Key Words ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation

An efficient mass propagation method for Feronia limonia was developed from excised shoot tips and nodal explants of in vitro grown seedlings. Explants were cultured on MS medium with different conc. of NAA, Kn, IAA and BAP singly or in combinations. Highest number of micro shoots and better plant growth were obtained from these two explants on MS medium supplemented with 0.2 mg/l BAP alone. The regenerated shoots were successfully rooted on MS medium supplemented with 0.5 mg/l NAA. The in vitro raised plantlets were successfully established in soil following the formation of roots with 100% survivability under ex vitro condition. Key words: Feronia limonia; Mass propagation; Node; Shoot tips; Multiple shoot DOI: 10.3329/bjsir.v45i1.5186 Bangladesh J. Sci. Ind. Res. 45(1), 75-78, 2010

scholarly journals In vitro Mass Propagation of Artemisia (Artemisia annua L.) cv: Anamed

2014 ◽  
Vol 23 (2) ◽  
pp. 165-176 ◽  
Author(s):  
Tilahun Hailu ◽  
Balcha Abera ◽  
Gabra Mariam
Keyword(s):  
Large Scale ◽  
Artemisia Annua ◽  
Shoot Tip ◽  
Nodal Explants ◽  
Mass Propagation ◽  
Mean Values ◽  
Coffee Husk ◽  
Soil Mix ◽  
Shoot Tip Explants

An efficient in vitro propagation protocol was developed for anamed (A-3) cultivar of Artemisia annua. Two and 1.5% concentration of NaOCl treatment for 10 and 20 min were found to be optimum for sterilization of shoot tip and nodal explants, respectively. Maximum percentage (98.75 ± 2.50) shoot induction was observed from nodal explants cultured on MS supplemented with 0.8 mg/l BAP + 0.1 mg/l IBA  followed by 82.50 ± 2.88% from shoot tip explants on the same medium with 0.8 mg/l TDZ for shoot tip explants. The highest number of shoots (8.05 ± 0.66/explant) was regenerated on MS + 1 mg/l BAP + 0.1 mg/l IBA. Best rooting with mean values of 18.25 ± 0.95/explant root number and root length (6.35 ± 0.10 cm) was recorded on 1/2 MS + 0.5 mg/l IBA. Up on acclimation and transplanting, 80% survival efficiency was observed on the soil mix ratio of  2 : 1 : 1 (decomposed coffee husk, forest soil and sand, respectively). The developed regeneration protocol enables a large scale commercial production and a possible system towards the genetic improvement of this crop. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17518 Plant Tissue Cult. & Biotech. 23(2): 165-176, 2013  (December)

scholarly journals Micropropagation of PLUCHEA LANCEOLATA (Oliver & Hiern.) Using Nodal Explant

2014 ◽  
Vol 22 (1) ◽  
pp. 35-39 ◽  
Author(s):  
Mafatlal M. Kher ◽  
Dimpal Joshi ◽  
Sureshkumar Nekkala ◽  
M. Nataraj ◽  
Dharmesh P. Raykundaliya
Keyword(s):  
Medicinal Plant ◽  
Salt Concentration ◽  
Nodal Explants ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Nodal Explant ◽  
Pluchea Lanceolata ◽  
Asteraceae Family ◽  
Important Medicinal Plant

AbstractPluchea lanceolata is an important medicinal plant of Asteraceae family known for its anti-arthritic and anti-inflammatory activity. A protocol was established for micropropagation of P. lanceolata using nodal explants. Nodal explants were inoculated onto Murashige and Skoog (1962) - MS medium supple–mented with 6-benzylaminopurine (BAP), kinetin (Kin), thidiazuron (TDZ) and 2iP (2-isopentenyladenine) at various concentrations (0.0, 0.5, 1.0, 1.5 and 2.0 mg·dm-3). The highest multiplication rate was obtained for nodal explants cultured on MS medium, supplemented with 0.5 mg·dm-3 thidiazuron (TDZ). In vitro raised shoots were successfully rooted on ½ mineral salt concentration of MS medium supplemented with 1.0 mg dm-3 IBA.

scholarly journals Impact of Hormones on the Proliferation of Shoots and Initiation of Roots in Salvia santolinifolia (Boiss), A High Value Medicinal Herb

2020 ◽  
Vol 63 (1) ◽  
pp. 30-36
Author(s):  
Tour Jan ◽  
Beena Naqvi ◽  
Ali Hazrat ◽  
Raiha Qadri ◽  
Muhammad Nisar ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
Butyric Acid ◽  
Medicinal Herb ◽  
In Vitro Conservation ◽  
Nodal Explants ◽  
Naphthalene Acetic Acid ◽  
Culture Age ◽  
Number Of Shoots

Salvia santolinifolia is a medicinal plant and an efficient in vitro conservation system is established. The influence of N6Benzylaminopurine (BAP), N6-(2-isopentyl)-adenine (2iP) and Kinetin at various concentrations were evaluated, single and in mixture with NAA (Naphthalene acetic acid) for the production of auxiliary shoots from nodal explants of S. santolinifolia. BAP at 3.0 mg/L in MS1 media produced maximum (11.66±3.38) number of shoots while elongated (5.37±1.45) shoots were produced in the MS2 medium in subcultures at 2.0 mg/L of 2iP. Least number of shoots were formed when auxin and cytokinin were used in combination. Length of culture, age was an important consideration for the initiation and development of roots. Rooting of shoot was attained with Indol-3-butyric acid (IBA) (3.0 mg/L) from shoots of 4th, 5th and 6th subculture while shoots taken from 1st, 2nd and 3rd subculture failed to form roots.  

scholarly journals In-vitro Callogenesis and Screening of Antimicrobial Activity of Callus and Seed of Caesalpinia bonducella F.: A Threatened Medicinal Plant of Western Ghats

2021 ◽  
pp. 76-89
Author(s):  
Rajani Shirsat ◽  
Ajit Kengar ◽  
Aruna Rai
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Plant Growth ◽  
Medicinal Plant ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Western Ghats ◽  
Callus Formation ◽  
Caesalpinia Bonducella

Aim: Caesalpinia bonducella Flem is a dioecious scrambling woody liana of Caesalpinoideae, a subfamily of Leguminosae. The plant is threatened and distributed in the deciduous forests of the Western Ghats of India. Being an important medicinal plant Caesalpinia bonducella F. attracted many scientists to exploit various activities associated with a number of phytoconstituents. The present study was undertaken to evaluate the most suitable media and suitable concentrations of plant growth regulators for in vitro Callogenesis and screening of antimicrobial activity of callus and seed of Caesalpinia bonducella. Materials and Methods: Callus was initiated from stem explants, on 1x and 0.5x MS medium plus supplements. The effects of plant growth regulators on callus cultures were studied and observations were made. The in vitro antibacterial activity was performed by using extracts of callus and seed of Caesalpinia bonducella in petroleum ether and methanol against multidrug resistance organisms. The organic extracts of seed and callus of the plant at concentrations of 0.02 mg/ml - 0.1 mg/ml were taken and their activities were measured.   Results: The combination of 2.5 mgL-1 2,4-D  with 2 mgL-1 BAP, resulted in the highest frequency and the highest mean percentage of callus formation (2.35 ± 0.294) with yellow friable callus. The results revealed that all the extracts had a variable degree of antibacterial activity. Conclusions: It was observed that 2,4-D at 2.5 mgL-1 in combination with BAP,2.0 mgL-1 BAP resulted in early initiation, highest induction percentage, with frequency highest mean percentage of callus formation, Antimicrobial tests with methanol and diethyl ether extract of Caesalpinia bonducella seed powder against the clinical isolates showed the zone of inhibition for all the pathogens tested with concentration of methanolic extract of C. bonducella seed powder.

scholarly journals In-Vitro Mass Propagation of Withania somnifera (L.) Dunal

1970 ◽  
Vol 11 ◽  
pp. 101-106 ◽  
Author(s):  
Durga Dutt Shukla ◽  
Nabin Bhattarai ◽  
Bijaya Pant
Keyword(s):  
Withania Somnifera ◽  
Callus Formation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Culture Technique ◽  
Nodal Explants ◽  
Ms Medium ◽  
Mass Propagation ◽  
Pharmaceutical Industries

Ashwagandha (Withania somnifera L.) Dunal] is an important medicinal plant and a major source of alkaloids and steroids (withanolids), which is regularly used in pharmaceutical industries. Various vegetative parts were studied for its mass propagation through tissue culture technique. Seeds were pretreated with GA3 (50 and 100 mgl-1) for 24 h and 80% germination was achieved. All the explants were taken from in-vitro germinated plant. Among the different explants tested, multiple shoot formation was achieved from shoot-tip and nodal explants in MS medium + 0.25, 0.5, and 1.0 mgl-1 kinetin. Nodal explants were selected for mass propagation protocol because it formed maximum number of shoots (16.25 shoots per explant) on MS medium + 1mgl-1 kinetin after eight weeks of culture. Increase in concentration of kinetin was most effective for callus formation. For further multiplication these shoots were sub-cultured on MS +0.5 mgl-1 kinetin. Presence of IAA at 0.5 mgl-1 was most effective medium for rooting of in-vitro propagated shoots. However, hardening was not achieved for these propagated plants. Key words: IAA; IBA; NAA; kinetin; in-vitro multiplication DOI: 10.3126/njst.v11i0.4131Nepal Journal of Science and Technology 11 (2010) 101-106

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