scholarly journals Micropropagation of PLUCHEA LANCEOLATA (Oliver & Hiern.) Using Nodal Explant

2014 ◽  
Vol 22 (1) ◽  
pp. 35-39 ◽  
Author(s):  
Mafatlal M. Kher ◽  
Dimpal Joshi ◽  
Sureshkumar Nekkala ◽  
M. Nataraj ◽  
Dharmesh P. Raykundaliya
Keyword(s):  
Medicinal Plant ◽  
Salt Concentration ◽  
Nodal Explants ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Nodal Explant ◽  
Pluchea Lanceolata ◽  
Asteraceae Family ◽  
Important Medicinal Plant

AbstractPluchea lanceolata is an important medicinal plant of Asteraceae family known for its anti-arthritic and anti-inflammatory activity. A protocol was established for micropropagation of P. lanceolata using nodal explants. Nodal explants were inoculated onto Murashige and Skoog (1962) - MS medium supple–mented with 6-benzylaminopurine (BAP), kinetin (Kin), thidiazuron (TDZ) and 2iP (2-isopentenyladenine) at various concentrations (0.0, 0.5, 1.0, 1.5 and 2.0 mg·dm-3). The highest multiplication rate was obtained for nodal explants cultured on MS medium, supplemented with 0.5 mg·dm-3 thidiazuron (TDZ). In vitro raised shoots were successfully rooted on ½ mineral salt concentration of MS medium supplemented with 1.0 mg dm-3 IBA.

scholarly journals In vitro propagation of Citrullus lanatus Thumb. from nodal explants culture

1970 ◽  
Vol 8 (2) ◽  
pp. 203-206 ◽  
Author(s):  
MM Khatun ◽  
MS Hossain ◽  
MA Haque ◽  
M Khalekuzzaman
Keyword(s):  
Citrullus Lanatus ◽  
In Vitro Regeneration ◽  
Shoot Proliferation ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Root Induction ◽  
Ms Medium ◽  
Nodal Explant ◽  
Grown Plant

A standard protocol was established for rapid in vitro propagation of watermelon (Citrullus lanatus Thumb.) from nodal explants of field grown plant. Multiple shoot proliferation was achieved from nodal explants on MS medium supplemented with 1.0 mg/l BAP + 0.2 mg/l NAA within 30 days of inoculation. The elongation of shoots was obtained on the same medium. Highest percentage of root induction was achieved on MS medium supplement with 1.0 mg/l IBA within 25 days of culture. Well rooted plantlets were transferred to small pots and after proper acclimatization the plantlets were transplanted in the field condition, where 80% plantlets were survived and grew successfully. Keywords: In vitro regeneration; Nodal explant; Citrullus lanatus DOI: 10.3329/jbau.v8i2.7926 J. Bangladesh Agril. Univ. 8(2): 203-206, 2010  

scholarly journals In vitro propagation of Nanorrhinum ramosissimum (Wall.) Betsche: A traditionally important medicinal plant

2021 ◽  
Vol 48 (3) ◽  
Author(s):  
Jyotsna Sharma ◽  
◽  
Anuja Koul ◽  
Savita Sharma ◽  
Raju Shankarayan ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Shoot Organogenesis ◽  
Germplasm Conservation ◽  
Ms Medium ◽  
In Vitro Plantlets ◽  
Half Strength ◽  
Shoot Tip Explants ◽  
Indole 3 Acetic Acid ◽  
Important Medicinal Plant

An efficient micropropagation protocol facilitates successful conservation and improvement of Nanorrhinum ramosissimum (Wall.) Betsche by biotechnological means. Shoot tip explants exhibited optimal organogenic response when inoculated on half-strength(1/2) Murashige and Skoog (MS) medium supplemented with kinetin (KN) and indole-3-acetic acid (IAA) (0.5 mg/L each). Shoot organogenesis was further enhanced when the multiplication medium was fortified with dextrose (1%) (2.6 shoots/explant; 7.9 cm shoot length). The regenerated shoots formed roots; however, the best rooting frequency (87%) was achieved on half-strength MS medium containing only IAA (0.5 mg/L). Four-week-old in vitro plantlets were acclimatized with 95% survival under greenhouse conditions. The regeneration protocol developed in this study can be utilized for germplasm conservation of this elite traditional medicinal plant.

Regeneration of Medicinal Plant Paederia foetida through Direct Organogenesis using Nodal Explants

2021 ◽  
Vol 24 (1) ◽  
pp. 89-98
Author(s):  
SH Binto ◽  
JU Ahmed ◽  
TK Ghosh
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
Ms Medium ◽  
Surface Sterilization ◽  
Paederia Foetida ◽  
Number Of Leaves ◽  
Number Of Shoots

Chinese fever vine (Paederia foetida L.), a valuable medicinal plant has been greatly utilized in therapeutic purposes throughout the world. Since conventional propagation techniques of P. foetida are very slow, inefficient and cannot cope with the increasing demand, in-vitro regeneration through tissue culture could be an alternative means of rapid propagation. Therefore, the efforts were made to develop a suitable protocol through direct organogenesis of P. foetida. After surface sterilization, the nodal explants were cultured in Murashigue and Skoog (MS) medium and MS medium supplemented with different concentrations and combinations of plant growth regulators. MS medium supplemented with 6-benzylaminopurine; BAP (2.0 mg L-1) produced the maximum number of shoots; 4.40 ± 0.98 and 5.40±1.12 after 15 and 30 days of culture respectively. The number of shoots gained by 15 days was found to be the highest; 1.20±0.80 at BAP (4.0 mg L-1) followed by 1.00±0.55 at BAP (2.0 mg L-1). Although the combination of BAP + Kinetin (2 mg L-1 +2 mg L-1) showed the highest shoot growth (3.40 ± 1.08 cm) by 15 days, sole application of BAP (2.0 mg L-1) or Kn (0.5, 1.0, 2.0 and 3.0 mg L-1) showed similar responses. BAP (2.0 mg L-1) showed the best responses for developing the highest number of leaves; 18.60 ± 2.42 and 29.20 ± 2.73 respectively after 15 and 30 days of culture. Similarly, development of the maximum number of leaves (10.60 ± 0.68) was reported by 15 days at BAP (2.0 mg L-1). Rooting was significantly induced in indole-3-acetic acid (IAA) supplemented to 1/2 strength MS medium as compared to control (only ½ strength MS medium). The best performance of rooting was observed by 0.5 mg L-1 IAA which produced average 4.33 roots per shoot after 21 days of culture. The regenerated plants showed similar morphology to the mother plants. Thus, a suitable protocol for successful multiplication of P. foetida in vitro was established using nodal explants. Ann. Bangladesh Agric. (2020) 24(1) : 88-98

scholarly journals Micropropagation Of Merremia Quinquefolia (L.) Hallier F. From Nodal Explants

2015 ◽  
Vol 23 (1) ◽  
pp. 13-16
Author(s):  
Mafatlal M. Kher ◽  
M. Nataraj ◽  
Hettal D. Parmar ◽  
Hasmatbanu Buchad
Keyword(s):  
Shoot Multiplication ◽  
Nodal Explants ◽  
Ms Medium ◽  
Mineral Salts ◽  
Single Node ◽  
The Family ◽  
Sedative Effects ◽  
Bud Breaking ◽  
Important Medicinal Plant

AbstractMerremia quinquefolia, is an important medicinal plant of the family Convolvulaceae known for its vasoconstrictor, uterotonic, neurohormonic, sympathicolytic and sedative effects. In the present investigation effect of cytokinins 6-benzylaminopurine (BAP), kinetin (Kn) and thidiazuron (TDZ), at concentrations 1.0, 2.0, 3.0, 4.0 and 5.0 mg·dm−3 on in vitro shoot multiplication from nodal explants of M. quinquefolia was evaluated. Bud breaking and emergence of shoots started within 10-15 days of inoculation in all media containing cytokinin. Murashige and Skoog (MS) medium supplemented with 4.0 mg·dm−3 BAP resulted in maximum number of shoots from single node within 45 days. In vitro raised shoots were successfully rooted on ½ mineral salts of MS medium with 3% sucrose supplemented with 2.0 mg·dm−3 indole-3-butyric acid (IBA). This is the first report on in vitro propagation of Merremia quinquefolia. This study can be useful for development of micropropagation protocols for related taxa.

scholarly journals An efficient protocol for in vitro regeneration from the nodal explants of Withania coagulans (Stocks) Dunal: a valuable medicinal herb

2021 ◽  
Vol 117 (2) ◽  
pp. 1
Author(s):  
Pari DEHVARI-NAGAN ◽  
Hosein ABBASPOUR ◽  
Mohammad Hasan ASARE ◽  
Sara SAADATMAND
Keyword(s):  
Significant Contribution ◽  
In Vitro Regeneration ◽  
Shoot Length ◽  
Nodal Explants ◽  
Nodal Explant ◽  
Withania Coagulans ◽  
Shoot Number ◽  
Important Medicinal Plant ◽  
Rooting Response

<p>In order to develop a protocol for the effective micropropagation of the important medicinal plant Withania coagulans (Stocks) Dunal, the effects of different concentrations and combinations of growth regulators on the nodal explants in two independent experiments were investigated. For shooting, a MS medium fortified with different concentrations and combinations of IBA (0.01, 0.1 and 0.5 mg l-1), BA (0.5, 1 and 2 mg l-1), Kin (0.5 and 1 mg l-1), PG (0.5 mg l-1) and GA (0.5 mg l-1) was used and the highest shooting response, shoot number and shoot length were obtained in the MS + IBA (0.01 mg l-1) + BA (0.5 mg l-1) + PG (0.5 mg l-1) + GA (0.5 mg l-1) treatment. In the second experiment, the effect of MS supplemented with different combinations and concentrations of IBA (0.1, 0.5, 1 and 2 mg l-1), NAA (0.1 and 1 mg l-1) and PG (1 mg l-1) on rooting of the nodal explants was investigated, which showed that the highest rooting response (%) was observed in the MS fortified with NAA (0.1 mg l-1), NAA (1 mg l-1), NAA (0.1 mg l-1) + PG (1 mg l-1), and NAA (1 mg l-1) + PG (1 mg l-1) treatments, as well as the highest number of roots at NAA (0.1 mg l-1) and the highest root length at IBA (1 mg l-1). Our findings highlight a complete micropropagation method for W. coagulans from the nodal explant that can make a significant contribution to the development of W. coagulans material for medical applications.</p>

scholarly journals Studies on In vitro Callus Induction and Regeneration from Nodal Explants of Cassia alata L.

2013 ◽  
Vol 19 (1) ◽  
pp. 39-44 ◽  
Author(s):  
MF Hasan ◽  
MS Rahman ◽  
MS Hossain ◽  
M Rahman
Keyword(s):  
Callus Tissue ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Nodal Explants ◽  
Ms Medium ◽  
Nodal Explant ◽  
Cassia Alata ◽  
Friable Callus ◽  
Cut Surface

A successful protocol for adventitious shoot regeneration was developed from the nodal explant derived callus tissue of Cassia alata. Greenish friable callus was induced from the cut surface of the nodal explants on MS medium supplemented with 1.5 mgl-1 2,4-D within twenty days of inoculation. The calli differentiated into adventitious shoots when they were cultured on MS medium supplemented with 1.5 mgl-1 2,4-D and 0.5 mgl-1 Kn. In this treatment, the highest number of shoot induction per callus was 6.0 ±1.0. The callus derived shoots rooted on MS medium containing 1.0 mgl-1 IBA within ten days of culture. The in vitro grown plantlets were acclimatized and successfully transferred to natural condition with 80% survival.DOI: http://dx.doi.org/10.3329/pa.v19i1.17105 Progress. Agric. 19(1): 39 - 44, 2008 

scholarly journals MICROPROPAGATION OF AN ENDANGERED AND ENDEMIC MEDICINAL PLANT CAYRATIA PEDATA VAR. GLABRA

2020 ◽  
pp. 191-196
Author(s):  
SHARMILA S ◽  
RAMYA EK ◽  
MOWNIKA S ◽  
DHIVYA SM
Keyword(s):  
Medicinal Plant ◽  
Callus Formation ◽  
Naphthalene Acetic Acid ◽  
Stem Explants ◽  
Root Induction ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Mass Multiplication ◽  
Endemic Medicinal Plant

Objective: The objective of the present study was to develop standardization protocol for the successful in vitro mass propagation of Cayratia pedata var. glabra through leaf and stem explants, since it is a rare, endangered, and endemic medicinal plant using biotechnological involvements and to conserve this endangered species. Methods: The application of biotechnological principles for the establishment of micropropagation under in vitro conditions has been studied by following the methods. The explants, namely, leaf and stem harvested from in vivo plants were thoroughly washed and properly sterilized with sterilients. The explants were transferred to Murashige and Skoog (MS) medium supplemented with growth regulators 6-benzyladenine (BAP) and naphthalene acetic acid (NAA) in the concentration range of 0.5–3.0 mg/l which were tested for callus induction and morphogenesis. The elongated shoots were transferred to MS medium supplemented with NAA at different concentrations for root induction. Results: The explants collected from the field (shola) were treated in different steriliants with various concentrations at different time for sterilization. Among the various combinations tried, the Teepol treatment for 10 min followed by bavistin 20 min, antibiotics, namely, ampicillin and rifampicin for 20 min, 70% alcohol for 30 s, and 0.12 % HgCl2 for 3 min was found to be effective. The explants were cultured in MS medium supplemented with various concentrations of BAP and NAA. The results noted that an increase in the concentration of BAP concomitantly reduced the frequency of callus formation. The maximum callusing frequency and more number of shoot formation was observed in the lower concentration of BAP (0.5 mg/l) in combination with NAA (0.2 mg/l). The callus obtained from all the above combinations was sub-cultured on MS medium with same combinations of BAP and NAA. The maximum frequency of root formation in leaf callus was 85% and 75% in stem callus and both were achieved on MS medium with NAA (1 mg/l) after 2 weeks. Conclusions: The current investigation provides a competent in vitro propagation method for C. pedata var. glabra which could be commercialized for developing identical plants with high-quality mass multiplication rate and for better conservation of the germplasm. Both the methods described here are well suited for the mass multiplication of this critically endangered and endemic climber species.

scholarly journals In vitro propagation of Boerhaavia diffusa L.: An important medicinal plant of family Nyctagimaceae

2019 ◽  
Vol 79 (01) ◽  
Author(s):  
Ashu Pandey ◽  
Oshin Verma ◽  
Suresh Chand
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
Liquid Medium ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Half Strength ◽  
Boerhaavia Diffusa ◽  
Important Medicinal Plant

Boerhaavia diffusa L., also known as santhi, or punarnava is an important medicinal plant, belonging to the family Nyctaginaceae. This species is said to be distributed throughout Malwa plateau in central India, as per ayurvedic literature, but due to extensive commercial exploitation, the species has become vulnerable. For callus induction, leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of 2,4- dichlorophenoxy acetic acid (2, 4-D 2.26 µM-9.04 µM) and N 6-benzyladenine (BA 1.11 µM-4.44 µM), either alone or in combinations. Calli formed within 10-12 days of culture, followed by shoots regeneration within 20-25 days. Direct organogenesis was achieved from nodal explants in MS media fortified with 2,4-D (2.26 µM-9.04 µM) along with BA (1.11 µM-4.44 µM) within 20 days. Multiple shooting was observed during subculture of in vitro regenerated shoots when 2,4-D was replaced with α-naphthalene acetic acid (NAA). Rooting was achieved in MS medium fortified with 2.85 µM IAA, within 7-10 days and also on half strength MS medium containing 2.85 µM Indole-3-acetic acid (IAA). For hardening, regenerated plants, with roots (3-4cm) were initially maintained on half-strength MS liquid medium (MS1/2) without growth regulators followed by quarter strength MS (MS1/4) liquid medium for 10 days. For acclimatization sterile mixture of soil, sand and manure (2:1:1) was used. Survival rate of regenerated plants was nearly 100%.

scholarly journals In vitro propagation of an economically important medicinal plant Lawsonia inermis L. through nodal segments

2021 ◽  
Vol 13 (3) ◽  
pp. 897-906
Author(s):  
Amit ◽  
Rajkumar ◽  
Narender Singh
Keyword(s):  
Medicinal Plant ◽  
Growth Regulators ◽  
Nodal Segments ◽  
Ms Medium ◽  
Lawsonia Inermis ◽  
Indole Butyric Acid ◽  
Mass Multiplication ◽  
Half Strength ◽  
Important Medicinal Plant

The present investigation aimed to standardize efficient plant regeneration protocol through in vitro culture by using nodal segment for mass multiplication of Lawsonia inermis an economically important medicinal plant species. Mass multiplication of shoots induced on Murashige and Skoog (MS) medium supplemented with different growth regulators like auxins and cytokinins separately and in different combinations. The medium fortified with 6-Benzylaminopurine ( BAP) 1.0 mg/l + kinetin (KN) 1.5mg/l  explained best compared to all other combinations. In vitro raised plantlets were excised and transferred in half strength MS  medium supplemented with different growth regulators like Indole Butyric acid ( IBA)  and naphthalene acetic acid (NAA ) (0.5-3.0 mg/l) in an experiment that gave rise to rooting. The half strength of MS medium additive with IBA in separate and in different combinations with NAA concentrations (0.5-3.0 mg/l) supported root development. The best response of rooting was obtained on half MS medium fortified with 1.0 mg/l IBA. The regenerated plantlets were successfully transplanted to pots. Regenerants were transferred to the field conditions and recorded the survival rate.. Among all the carbon sources and gelling agents used, sucrose (3%) in combination with 0.8 per cent agar-agar has proved significantly better. Multiple shoots formation with longer shoots were achieved on medium with 1.0mg/l BAP and 1.5mg/l Kn. Thus, it is possible to develop a large number of plants of L. inermis through shoot bud regeneration which can cater for the need of pharmaceutical as well as other industries.

scholarly journals Development of a profused In vitro shoot multiplication using leaf explants of Bacopa monnieri (L.) Pennell

2020 ◽  
pp. 14-17
Author(s):  
Sape Subba Tata
Keyword(s):  
Medicinal Plant ◽  
Leaf Explants ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Bacopa Monnieri ◽  
Optimum Concentration ◽  
Ms Medium ◽  
Efficient Regeneration ◽  
Important Medicinal Plant

Bacopa monnieri (L.) Pennell is an important medicinal plant used for the preparation of medhyarasayan (rasayana). Leaf explants of field grown young plants of B. monnieri was used to establish an efficient regeneration protocol with cytokinin (BAP) and auxin (IAA). The highest multiplication, i.e. (220 shoots/leaf, a cumulative of 2200 shoots from 10 explants) were noticed after 45 days of culture in MS medium supplemented with BAP(1.5mg/L) and IAA(0.5mg/L). The optimum concentration of growth regulator for shoot elongation and rooting was recorded in MS+GA3(0.25mg/L) and MS+IBA(1.5mg/L) respectively. The rooted plantlets were successfully established in green house conditions.

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