scholarly journals Discovery and Functional Study of a Novel Genomic Locus Homologous to Bα-Mating-Type Sublocus of Lentinula edodes

Mycobiology ◽  
2021 ◽  
pp. 1-7
Author(s):  
Yun Jin Lee ◽  
Eunbi Kim ◽  
Hyerang Eom ◽  
Seong-Hyeok Yang ◽  
Yeon Jae Choi ◽  
...  
Keyword(s):  
Mating Type ◽  
Lentinula Edodes ◽  
Functional Study ◽  
Genomic Locus

Genetic analysis of segregation distortion of mating-type factors in Lentinula edodes

2005 ◽  
Vol 15 (8) ◽  
pp. 684-688
Author(s):  
Cheng Shuiming ◽  
Lin Fanxue ◽  
Xu Xuefeng ◽  
Li Anzheng ◽  
Lin Fangcan
Keyword(s):  
Genetic Analysis ◽  
Mating Type ◽  
Segregation Distortion ◽  
Lentinula Edodes

scholarly journals Investigation of Mating Pheromone–Pheromone Receptor Specificity in Lentinula edodes

Genes ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 506
Author(s):  
Sinil Kim ◽  
Byeongsuk Ha ◽  
Minseek Kim ◽  
Hyeon-Su Ro
Keyword(s):  
Mating Type ◽  
Lentinula Edodes ◽  
Signal Pathway ◽  
Recombinant Yeast ◽  
Mating Pheromone ◽  
Type Locus ◽  
Pheromone Receptor ◽  
Mating Type Locus ◽  
Yeast Mating ◽  
Mating Signal

The B mating-type locus of Lentinula edodes, a representative edible mushroom, is highly complex because of allelic variations in the mating pheromone receptors (RCBs) and the mating pheromones (PHBs) in both the Bα and Bβ subloci. The complexity of the B mating-type locus, five Bα subloci with five alleles of RCB1 and nine PHBs and three Bβ subloci with 3 alleles of RCB2 and five PHBs, has led us to investigate the specificity of the PHB–RCB interaction because the interaction plays a key role in non-self-recognition. In this study, the specificities of PHBs to RCB1-2 and RCB1-4 from the Bα sublocus and RCB2-1 from the Bb sublocus were investigated using recombinant yeast strains generated by replacing STE2, an endogenous yeast mating pheromone receptor, with the L. edodes RCBs. Fourteen synthetic PHBs with C-terminal carboxymethylation but without farnesylation were added to the recombinant yeast cells and the PHB–RCB interaction was monitored by the expression of the FUS1 gene—a downstream gene of the yeast mating signal pathway. RCB1-2 (Bα2) was activated by PHB1 (4.3-fold) and PHB2 (2.1-fold) from the Bα1 sublocus and RCB1-4 (Bα4) was activated by PHB5 (3.0-fold) and PHB6 (2.7-fold) from the Bα2 sublocus and PHB13 (3.0-fold) from the Bα5 sublocus. In particular, PHB3 from Bβ2 and PHB9 from Bβ3 showed strong activation of RCB2-1 of the Bβ1 sublocus by 59-fold. The RCB–PHB interactions were confirmed in the monokaryotic S1–10 strain of L. edodes by showing increased expression of clp1, a downstream gene of the mating signal pathway and the occurrence of clamp connections after the treatment of PHBs. These results indicate that a single PHB can interact with a non-self RCB in a sublocus-specific manner for the activation of the mating pheromone signal pathways in L. edodes.

Mating-Type Incompatibility between Commercial Strains of Lentinula edodes

1994 ◽  
Vol 18 (2) ◽  
pp. 95-102 ◽  
Author(s):  
Hilary M Fox ◽  
John Burden ◽  
Shu-Ting Chang ◽  
John F Peberdy
Keyword(s):  
Mating Type ◽  
Lentinula Edodes

The genetic structure of the A mating-type locus of Lentinula edodes

Gene ◽  
2014 ◽  
Vol 535 (2) ◽  
pp. 184-190 ◽  
Author(s):  
Chun Hang Au ◽  
Man Chun Wong ◽  
Dapeng Bao ◽  
Meiyan Zhang ◽  
Chunyan Song ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Mating Type ◽  
Lentinula Edodes ◽  
Type Locus ◽  
Mating Type Locus

Cloning and identification of partial DNA fragment for the B mating-type factor in Lentinula edodes using degenerate PCR

2006 ◽  
Vol 23 (3) ◽  
pp. 411-415 ◽  
Author(s):  
An-Zheng Li ◽  
Xue-Feng Xu ◽  
Fan-Xue Lin ◽  
Shui-Ming Cheng ◽  
Fang-Can Lin
Keyword(s):  
Mating Type ◽  
Lentinula Edodes ◽  
Degenerate Pcr ◽  
Dna Fragment ◽  
Type Factor

Molecular analysis of B mating type diversity in Lentinula edodes

2019 ◽  
Vol 243 ◽  
pp. 55-63 ◽  
Author(s):  
Byeongsuk Ha ◽  
Yoon Jung Moon ◽  
Yelin Song ◽  
Sinil Kim ◽  
Minseek Kim ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Mating Type ◽  
Lentinula Edodes

Diversity of A mating type in Lentinula edodes and mating type preference in the cultivated strains

2018 ◽  
Vol 56 (6) ◽  
pp. 416-425 ◽  
Author(s):  
Byeongsuk Ha ◽  
Sinil Kim ◽  
Minseek Kim ◽  
Yoon Jung Moon ◽  
Yelin Song ◽  
...  
Keyword(s):  
Mating Type ◽  
Lentinula Edodes

Cloning of the Lentinula edodes B mating-type locus and identification of the genetic structure controlling B mating

Gene ◽  
2013 ◽  
Vol 531 (2) ◽  
pp. 270-278 ◽  
Author(s):  
Lin Wu ◽  
Arend van Peer ◽  
Wenhua Song ◽  
Hong Wang ◽  
Mingjie Chen ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Mating Type ◽  
Lentinula Edodes ◽  
Type Locus ◽  
Mating Type Locus

Sequence characterized amplified region markers tightly linked to the mating factors ofLentinula edodes

Genome ◽  
2004 ◽  
Vol 47 (1) ◽  
pp. 156-162 ◽  
Author(s):  
Ayako Tanaka ◽  
Kazuhiro Miyazaki ◽  
Haruki Murakami ◽  
Susumu Shiraishi
Keyword(s):  
Mating Type ◽  
Lentinula Edodes ◽  
Mating Types ◽  
Randomly Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Identification Procedure ◽  
Polymerase Chain Reaction Condition ◽  
Sequence Characterized Amplified Region ◽  
Polymerase Chain ◽  
Mating Factor

Detecting the mating types in shiitake, Lentinula edodes (Berk.) Pegler, is important for making progress in the breeding of this mushroom and determining the compatibility of the pair to cross. Shiitake is a tetrapolar fungus with two unlinking mating factors, A factor and B factor. We screened molecular markers linked to the mating factors using the randomly amplified polymorphic DNA (RAPD) method to develop the mating type identification procedure. Using 147 oligonucleotide primers, a total of 6 linkage markers for the shiitake mating factors, 4 markers for the A factor and 2 markers for the B factor, were discovered with a logarithm of the odds threshold of 3.0 for linkage. Two RAPDs that perfectly segregated with each mating factor among 72 basidiospore strains were detected. Both of these RAPDs were cloned and sequenced to convert them to the sequence characterized amplified region (SCAR) markers. Four primers, two sets of primers, were designed according to the internal sequences of two RAPDs tightly linking to the A factor or B factor. Consequently, we determined the polymerase chain reaction condition for multiplex analyses of these SCAR markers.Key words: Lentinula edodes, SCAR, diagnostic, mating type.

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