Molecular phylogenetic and morphological evidence reveal a rare limacoid snail genus, Khmerquantula gen. nov. (Eupulmonata: Dyakiidae) from Cambodia

Forty-nine years after the last description of a slippery frog, we describe a seventh species of the genus Conraua. The new Conraua is endemic to the Atewa Range Forest Reserve, central Ghana, and is described based on genetic, bioacoustics, and morphological evidence. Recent molecular phylogenetic and species delimitation analyses support this population as distinct from nominotypical C. derooi in eastern Ghana and adjacent Togo. The new species is sister to C. derooi, from which it differs ~4% in the DNA sequence for mitochondrial ribosomal 16S. Genetic divergences in 16S to other species of Conraua range from 4–12%. The new species is distinguished morphologically from its congeners, including C. derooi, by the combination of the following characters: medium body size, robust limbs, lateral dermal fringing along edges of fingers, cream ventral color with brown mottling, the presence of a lateral line system, indistinct tympanum, the presence of inner, outer, and middle palmar tubercles, and two subarticular tubercles on fingers III and IV. We compare the advertisement calls of the new species with the calls from C. derooi and find that they differ by duration, frequency modulation, and dominant frequency. We discuss two potential drivers of speciation between C. derooi and the new species, including river barriers and fragmentation of previously more widespread forests in West Africa. Finally, we highlight the importance of the Atewa Range Forest Reserve as a critical conservation area within the Upper Guinean biodiversity hotspot.

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An enigma no more: an integrated taxonomic revision of Aenigmetopia Malloch reveals novel phylogenetic placement and four new species (Diptera : Sarcophagidae : Miltogramminae)

Invertebrate Systematics ◽

10.1071/is19051 ◽

2020 ◽

Author(s):

Nikolas P. Johnston ◽

James F. Wallman ◽

Krzysztof Szpila ◽

Thomas Pape

Keyword(s):

New Species ◽

Taxonomic Revision ◽

Single Species ◽

Species Level ◽

Morphological Data ◽

Morphological Evidence ◽

Phylogenetic Placement ◽

Molecular Phylogenetic ◽

First Time ◽

Flesh Flies

Aenigmetopia Malloch is the only endemic genus of miltogrammine flesh flies (Diptera:Sarcophagidae) in Australia and, until now it has been known from a single species, A. fergusoni Malloch. This study constitutes the first comprehensive taxonomic revision of Aenigmetopia. Four new species, Aenigmetopia amissa, sp. nov., A. corona, sp. nov., A. kryptos, sp. nov. and A. pagoni, sp. nov., are described through the integration of molecular and morphological data and characters for genus- and species-level diagnoses are given. Aenigmetopia is included in a molecular phylogenetic analysis for the first time and the genus emerges as the sister taxon to Metopia Meigen, in agreement with morphological evidence.

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A new family of Laniatores (Arachnida:Opiliones) from the Afrotropics

Invertebrate Systematics ◽

10.1071/is11003 ◽

2011 ◽

Vol 25 (2) ◽

pp. 143 ◽

Cited By ~ 21

Author(s):

Prashant P. Sharma ◽

Carlos E. Prieto ◽

Gonzalo Giribet

Keyword(s):

Canary Islands ◽

Morphological Evidence ◽

Monotypic Genus ◽

Sister Relationship ◽

New Family ◽

Phylogenetic Studies ◽

Biogeographic Provinces ◽

The Family ◽

Molecular Phylogenetic

Among Opiliones, Afrotropical lineages constitute some of the least studied groups in comparison with those endemic to other biogeographic provinces. Based upon morphological evidence, we erect Pyramidopidae, fam. nov. to distinguish a group of Laniatores from the family Phalangodidae. We review evidence from recent molecular phylogenetic studies that corroborate the independence of Pyramidopidae, fam. nov. from previously described families and support its sister relationship to another largely Afrotropical group, the family Assamiidae. The monotypic genus Maiorerus Rambla, 1993 is transferred to Pyramidopidae, fam. nov. The new family comprises 12 genera geographically restricted to Africa and the adjacent Canary Islands. Interfamilial relationships of the derived Laniatores are discussed in the context of gross and genitalic morphology.

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A classification of Lejeuneaceae (Marchantiophyta) based on molecular and morphological evidence

Phytotaxa ◽

10.11646/phytotaxa.100.1.2 ◽

2013 ◽

Vol 100 (1) ◽

pp. 6 ◽

Cited By ~ 30

Author(s):

S. ROBBERT GRADSTEIN

Keyword(s):

Molecular Methods ◽

Morphological Evidence ◽

The Family ◽

Molecular Phylogenetic

Lejeuneaceae are the largest family of the liverworts with at least one thousand species in 68 currently accepted genera. The number of genera is much lower than accepted previously and was reduced based on recent molecular work. This paper present a first classification of Lejeuneaceae based on integrated molecular-phylogenetic and morphological evidence. The family is subdivided into two broad subfamilies, Ptychanthoideae (19 genera) and Lejeuneoideae (49 genera). Ptychanthoideae are not further subdivided whereas Lejeuneoideae are classified into three tribes: Brachiolejeuneeae (8 genera), Symbiezidieae (new; 1 genus) and Lejeuneeae (40 genera). Lejeuneeae, the largest tribe in the family, are classified into eight subtribes: Ceratolejeuneinae (2 genera), Cheilolejeuneinae (4 genera), Cololejeuneinae (12 genera), Cyclolejeuneinae (3 genera), Drepanolejeuneinae (2 genera), Echinolejeuneinae (3 genera), Lejeuneinae (5 genera) and Lepidolejeuneinae (2 genera). Seven genera of Lejeuneeae have not yet been studied by molecular methods and are not classified.

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A revision of the new genus Amiga Nakahara, Willmott & Espeland, gen. n., described for Papilio arnaca Fabricius, 1776 (Lepidoptera, Nymphalidae, Satyrinae)

ZooKeys ◽

10.3897/zookeys.821.31782 ◽

2019 ◽

Vol 821 ◽

pp. 85-152 ◽

Cited By ~ 6

Author(s):

Shinichi Nakahara ◽

Gerardo Lamas ◽

Stephanie Tyler ◽

Mario Alejandro Marín ◽

Blanca Huertas ◽

...

Keyword(s):

Central America ◽

New Taxa ◽

New Genus ◽

Common Species ◽

Morphological Evidence ◽

Monotypic Genus ◽

Southern Brazil ◽

Southern Mexico ◽

New Subspecies ◽

Molecular Phylogenetic

We here propose a new, monotypic genus, Amiga Nakahara, Willmott & Espeland, gen. n., to harbor a common Neotropical butterfly, described as Papilioarnaca Fabricius, 1776, and hitherto placed in the genus Chloreuptychia Forster, 1964. Recent and ongoing molecular phylogenetic research has shown Chloreuptychia to be polyphyletic, with C.arnaca proving to be unrelated to remaining species and not readily placed in any other described genus. Amigaarnacagen. n. et comb. n. as treated here is a widely distributed and very common species ranging from southern Mexico to southern Brazil. A neotype is designated for the names Papilioarnaca and its junior synonym, Papilioebusa Cramer, 1780, resulting in the treatment of the latter name as a junior objective synonym of the former. A lectotype is designated for Euptychiasericeella Bates, 1865, which is treated as a subspecies, Amigaarnacasericeella (Bates, 1865), comb. n. et stat. n., based on molecular and morphological evidence. We also describe two new taxa, Amigaarnacaadela Nakahara & Espeland, ssp. n. and Amigaarnacaindianacristoi Nakahara & Marín, ssp. n., new subspecies from the western Andes and eastern Central America, and northern Venezuela, respectively.

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Transfer of Lejeunea huctumalcensis to Physantholejeunea (Lejeuneaceae, Porellales)

Australian Systematic Botany ◽

10.1071/sb13039 ◽

2013 ◽

Vol 26 (5) ◽

pp. 386 ◽

Cited By ~ 9

Author(s):

Aleksandra Czumay ◽

Shanshan Dong ◽

Armin Scheben ◽

Alfons Schäfer-Verwimp ◽

Kathrin Feldberg ◽

...

Keyword(s):

Chloroplast Genome ◽

Phylogenetic Analyses ◽

Morphological Evidence ◽

Its2 Region ◽

Rbcl Gene ◽

Molecular Phylogenetic

Phylogenetic analyses of a three-marker dataset of Lejeuneaceae (chloroplast genome rbcL gene and trnL–trnF region, and nuclear ribosomal ITS1–5.8S-ITS2 region) resolve Lejeunea huctumalcensis (synonym Ceratolejeunea dussiana) in a well supported lineage with Physantholejeunea portoricensis. Representatives of Lejeunea and Ceratolejeunea form independent lineages. Physantholejeunea and L. huctumalcensis share the presence of ocelli, pycnolejeuneoid innovations and keeled perianths, with keels forming horn-like projections. On the basis of the molecular phylogenetic and morphological evidence, we transfer L. huctumalcensis to Physantholejeunea.

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Molecular phylogenetic and morphological evidence for the congeneric status ofGoniothalamusandRichella(Annonaceae)

Taxon ◽

10.1002/tax.581013 ◽

2009 ◽

Vol 58 (1) ◽

pp. 127-132 ◽

Cited By ~ 10

Author(s):

Maliwan Nakkuntod ◽

Yvonne C.F. Su ◽

Tosak Seelanan ◽

Richard M.K. Saunders

Keyword(s):

Morphological Evidence ◽

Molecular Phylogenetic

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Phylogenetic analysis and systematic position of two new species of the ant genus Crematogaster (Hymenoptera, Formicidae) from Southeast Asia

European Journal of Taxonomy ◽

10.5852/ejt.2017.370 ◽

2017 ◽

Cited By ~ 1

Author(s):

Shingo Hosoishi ◽

Kazuo Ogata

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Divergence Time ◽

Morphological Characters ◽

Systematic Position ◽

Species Group ◽

Morphological Evidence ◽

Divergence Time Estimates ◽

Close Relationship ◽

Molecular Phylogenetic

Two distinct new species of the ant genus Crematogaster, C. khmerensis sp. nov. and C. pfeifferi sp. nov., are described from Cambodia and Malaysia, respectively. The two species are unique among Asian Crematogaster in that they have vertically directed propodeal spines, but their systematic positions have not been determined based on morphological characters alone. Molecular phylogenetic analysis of 89 Crematogaster taxon matrices previously published plus C. khmerensis sp. nov., using nuclear genes, reveals that C. khmerensis sp. nov. is nested within the Australo-Asian Crematogaster clade. Morphological assignment of the developed pronotal shoulders implies a close relationship between C. khmerensis sp. nov. and the C. tetracantha-group. Based on molecular and morphological evidence, we erect a new species group, C. khmerensis-group, to contain C. khmerensis sp. nov. and C. pfeifferi sp. nov. Divergence time estimates using MCMCTree shows that the root node of the C. khmerensis sp. nov. terminal is estimated to be of middle Miocene age at 15 million years old. The position of the C. khmerensis-group well supports the Oriental- to Australian-region dispersal history that has been proposed for the Australo-Asian Crematogaster clade.

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A new species of Potentilla (Potentilleae, Rosaceae) from central China, with reference to molecular and morphological evidence

Phytotaxa ◽

10.11646/phytotaxa.480.2.7 ◽

2021 ◽

Vol 480 (2) ◽

pp. 185-194

Author(s):

HUA-JIE ZHANG ◽

DAI-GUI ZHANG ◽

TAO FENG ◽

QUN LIU ◽

JING-YUAN YANG ◽

...

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Wild Population ◽

Hubei Province ◽

Central China ◽

Morphological Evidence ◽

Dense Array ◽

Molecular Phylogenetic ◽

A New Species ◽

Forest District

Potentilla sunhangii, a new species of Potentilla, from the Jin-hou-ling Mountains, Shen-nong-jia Forest District of Hubei province, China, is described and illustrated. The wild population of the new species is at an elevation of approximately 2930 m and has leaves palmately 3 foliolate. It is morphologically much similar to P. saundersiana, whilst being easily distinguished by its dense array of glands around the plant and green on both surfaces of leaves. Our molecular phylogenetic analysis based on nuclear and chloroplast confirmed the species belongs to Potentilla, in Argentea clade.

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Molecular phylogenetic analysis of the catfish species Auchenoglanis occidentalis (Valenciennes, 1840) (Pisces: Claroteidae) from Lake Turkana in East Africa: taxonomic implications

Zootaxa ◽

10.11646/zootaxa.4450.1.8 ◽

2018 ◽

Vol 4450 (1) ◽

pp. 115

Author(s):

BRIAN OKWIRI ◽

LIANG CAO ◽

DOROTHY WANJA NYINGI ◽

E. ZHANG

Keyword(s):

Phylogenetic Analysis ◽

Distinct Species ◽

Coi Gene ◽

Valid Species ◽

Morphological Evidence ◽

African Catfish ◽

Lake Turkana ◽

The Family ◽

Molecular Phylogenetic ◽

Taxonomic Implications

Cytochrome c oxidase subunit I (COI) gene sequences of two specimens here recognized as Auchenoglanis occidentalis from Lake Turkana in the Ethiopian section were determined. A COI gene-based phylogenetic analysis was performed for these along with sequences of African catfish species from the family Clarotidae available in GenBank. Based on results of this analysis, it is concluded that (1) the currently identified A. occidentalis is a species complex that includes several distinct species; (2) the Niger River basin harbors two distinct species of Auchenoglanis, one of which occurs in Lake Turkana, as well as A. biscutatus; and (3) A. sacchii is likely a valid species, but it is not the endemic species of Lake Turkana. It is suggested here that species diversity of Auchenoglanis requires further study based on molecular and morphological evidence.

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