Garcinielliptone FC, a polyisoprenylated benzophenone fromPlatonia insignisMart., promotes vasorelaxant effect on rat mesenteric artery

2014 ◽  
Vol 28 (12) ◽  
pp. 923-927 ◽  
Author(s):  
Daniel Dias Rufino Arcanjo ◽  
Joaquim Soares da Costa-Júnior ◽  
Lucas Henrique Porfírio Moura ◽  
Alexandre Barros Falcão Ferraz ◽  
Raíssa Rebés Rossatto ◽  
...  
Keyword(s):  
Mesenteric Artery ◽  
Vasorelaxant Effect ◽  
Rat Mesenteric Artery

scholarly journals Vasorelaxant Effect Induced by the Essential Oil of Ocotea duckei Vattimo Leaves and Its Main Constituent, Trans-caryophyllene, in Rat Mesenteric Artery

2020 ◽  
pp. 31-39
Author(s):  
Tays Amanda Felisberto Gonçalves ◽  
Renildo Moura da Cunha ◽  
Dionatas Ulises de Oliveira Meneguetti ◽  
Marcio Roberto Viana Santos ◽  
José Maria Barbosa- Filho ◽  
...  
Keyword(s):  
Essential Oil ◽  
Mesenteric Artery ◽  
Calcium Release ◽  
Mesenteric Arteries ◽  
Dependent Manner ◽  
Main Constituent ◽  
Response Curves ◽  
Vasorelaxant Effect ◽  
Rat Mesenteric Artery ◽  
Isolated Rat

Aims: To evaluate the vasorelaxant effect induced by the essential oil of the leaves of O. duckei Vattimo (ODEO) and its main constituent, trans-caryophyllene, in rat superior mesenteric arteries. Methodology: Isolated rat superior mesenteric rings were suspended by cotton threads for isometric tension recordings in Tyrode’s solution at 37ºC, gassed with 95% O2 and 5% CO2 and different ODEO concentrations (0.1-300 μg/mL) or trans-caryophyllene (1-1000 μg/mL) were added cumulatively to the organ baths. Results: Vasorelaxant effect induced by the essential oil of Ocotea duckei leaves (ODEO) and its main constituent, trans-caryophyllene (60.54 %), was evaluated in this work. In intact isolated rat superior mesenteric rings ODEO (0.1-300 μg/mL, n=6) induced concentration-dependent relaxation of tonus induced by phenylephrine (10 µM) or K+-depolarizing solution (KCl 80 mM) (IC50=31±5, 5±0.4 µg/mL, respectively, n=6). The relaxations of phenylephrine-induced contractions were not significantly attenuated after removal of the vascular endothelium (IC50=25±5 µg/mL). ODEO antagonized the concentration-response curves to CaCl2 (10-6-3x10-2 M) and Bay K 8644 (10-10-3x10-6 M). Furthermore, in nominally without calcium solution, ODEO significantly inhibited, in a concentration-dependent manner, transient contractions induced by 10 µM phenylephrine or 20 µM caffeine. Trans-caryophyllene induced vasorelaxations, however, this effect was 18.6 times less potent when compared to ODEO-induced vasorelaxations. Conclusion: The relaxant effect induced by ODEO in rat superior mesenteric artery rings is endothelium-independent and seems to be related to both, inhibition of Ca2+ influx through L-type voltage-gated Ca2+-channels sensitive to dihydropyridines and inhibition of the calcium release from intracellular IP3-and caffeine-sensitive stores.

scholarly journals Vasorelaxant effect of Hyptis fruticosa Salzm. ex Benth., Lamiaceae, dichloromethane extract on rat mesenteric artery

2010 ◽  
Vol 20 (5) ◽  
pp. 762-765 ◽  
Author(s):  
Ítalo J. A. Moreira ◽  
Maria P. N. Moreno ◽  
Maria F. G. Fernandes ◽  
João B. Fernandes ◽  
Flávia V. Moreira ◽  
...  
Keyword(s):  
Mesenteric Artery ◽  
Vasorelaxant Effect ◽  
Rat Mesenteric Artery

Comparison of vasorelaxant effect and tolerance profile of a novel isosorbide-5-mononitrate derivative with its stereoisomer and parent drug on rat mesenteric artery

2011 ◽  
Vol 60 (04) ◽  
pp. 189-197
Author(s):  
Radica Stepanović-Petrović ◽  
Vladimir Savić ◽  
Maja Tomić ◽  
Zorana Tokic-Vujošević ◽  
Milena Simić ◽  
...  
Keyword(s):  
Mesenteric Artery ◽  
Parent Drug ◽  
Vasorelaxant Effect ◽  
Rat Mesenteric Artery

Vasorelaxant Action of N-p-Nitrophenylmaleimide in the Isolated Rat Mesenteric Artery

2010 ◽  
Vol 65 (7-8) ◽  
pp. 451-457 ◽  
Author(s):  
Eurica Ribeiro ◽  
Fabíola F. Furtado ◽  
Vânia F. Noldin ◽  
Rogério Corrêa ◽  
Valtir Cechinel-Filho ◽  
...  
Keyword(s):  
Mesenteric Artery ◽  
Inhibitory Effect ◽  
Isometric Tension ◽  
Free Medium ◽  
Response Curves ◽  
Tetraethylammonium Bromide ◽  
Relaxant Effect ◽  
Vasorelaxant Effect ◽  
Resting Tension ◽  
Rat Mesenteric Artery

The vasorelaxant response of N-p-nitrophenylmaleimide (4-NO2-NPM) was evaluated. The mesenteric rings (1 - 2 mm i.d.) were suspended by cotton thread for isometric tension recordings in a Tyrode’s solution at 37 °C and gassed with a mixture of 95% O2 and 5% CO2, under a resting tension of 0.75 g. 4-NO2-NPM induced relaxation in mesenteric rings pre-contracted with phenylephrine (Phe; 10 μM, pD2 = 6.7 ± 0.3) or KCl (80 mM, pD2 = 3.9 ± 0.2). This effect was significantly attenuated after removal of the vascular endothelium, NG-nitro L-arginine methyl ester (L-NAME; 100 μM), atropine (1 μM), indomethacin (10 μM), L-NAME + indomethacin or 1H-[1,2,3]oxadiazolo[4,3-α]quinoxalin-1-one (ODQ; 10 μM). LArginine (1 mM) reversed the inhibitory effect of L-NAME. In endothelium-intact preparations pre-incubated with 20 mM KCl, tetraethylammonium bromide (TEA; 1 mM) or glibenclamide (Glib; 10 μM), the vasorelaxant effect was significantly attenuated when compared to controls (endothelium intact). In denuded rings, separate incubation with 20 mM KCl, TEA or Glib did not change the relaxation when compared with that obtained in denuded rings. 4-NO2-NPM inhibited in a concentration-dependent and non-competitive manner the concentration-response curves induced by CaCl2. In calcium-free medium, the transient contractions induced by Phe (10 μM) or caffeine (20 mM) were inhibited. The relaxant effect induced by 4-NO2 -NPM appeared to be due to endothelial muscarinic receptors activation, NO and prostacyclin release and KATP and BKCa (Ca2+-activated K+ channels) endotheliumdependent activation. Inhibition of the Ca2+ influx and inhibition of the Ca2+ release from intracellular IP3- and caffeine-sensitive stores are also involved in the vasorelaxation

scholarly journals Involvement of Potassium Channels in Vasorelaxant Effect Induced byValeriana prionophyllaStandl. in Rat Mesenteric Artery

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Milena Ramos Reis ◽  
Abrahão Alves de Oliveira Filho ◽  
Lilia Simone Urzedo Rodrigues ◽  
Jaíse Paiva Araújo ◽  
Priscilla Maria Pereira Maciel ◽  
...  
Keyword(s):  
Mesenteric Artery ◽  
Barium Chloride ◽  
Vasorelaxant Effect ◽  
Rat Mesenteric Artery ◽  
Vascular Component ◽  
Vasorelaxant Activity ◽  
Relaxation Maximum ◽  
Endothelium Dependent Relaxation

Assaysin vitroandin vivowere performed on extract from roots and leaves from theValeriana prionophyllaStandl. (VPR and VPF, resp.). In phenylephrine (1 μM) precontracted rings, VPR (0.01–300 μg/mL) induced a concentration-dependent relaxation (maximum response (MR) = 75.4 ± 4.0%, EC50= 5.97 (3.8–9.3)μg/mL,n=6]); this effect was significantly modified after removal of the endothelium (EC50= 39.6 (27.2–57.6)μg/mL,P<0.05). However, VPF-induced vasorelaxation was less effective compared to VPR. When rings were preincubated with L-NAME (100 μM) or indomethacin (10 μM), the endothelium-dependent relaxation induced by VPR was significantly attenuated (MR = 20.9 ± 2.3%, 34.2 ± 2.9%, resp.,P<0.001). In rings denuded endothelium, precontracted with KCl (80 mM), or in preparations pretreated with KCl (20 mM) or tetraethylammonium (1 or 3 mM), the vasorelaxant activity of VPR was significantly attenuated (MR = 40.0 ± 8.2,n=5; 50.5 ± 6.0%; 49.3 ± 6.4%; 46.8 ± 6.2%; resp.,P<0.01). In contrast, neither glibenclamide (10 μM), barium chloride (30 μM), nor 4-aminopyridine (1 mM) affected VPR-induced relaxation. Taken together, these results demonstrate that hypotension induced by VPR seems to involve, at least in part, a vascular component. Furthermore, endothelium-independent relaxation induced by VPR involves K+channels activation, most likely due to BKCachannels, in the rat superior mesenteric artery.

Effects of magnesium on the vasoconstrictor responses to norepinephrine and potassium chloride in the rat mesenteric artery.

1982 ◽  
Vol 23 (5) ◽  
pp. 783-789
Author(s):  
Hiromichi SUZUKI ◽  
Kazuoki KONDO ◽  
Michiko HANDA ◽  
Takao SARUTA
Keyword(s):  
Potassium Chloride ◽  
Mesenteric Artery ◽  
Rat Mesenteric Artery

Large-conductance Ca2+-activated K+ currents blocked and impaired by homocysteine in human and rat mesenteric artery smooth muscle cells

Life Sciences ◽  
2007 ◽  
Vol 80 (22) ◽  
pp. 2060-2066 ◽  
Author(s):  
Benzhi Cai ◽  
Dongmei Gong ◽  
Zhenwei Pan ◽  
Yu Liu ◽  
Hong Qian ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Mesenteric Artery ◽  
Muscle Cells ◽  
Rat Mesenteric Artery ◽  
K Currents

Functional changes in adenylyl cyclases and associated decreases in relaxation responses in mesenteric arteries from diabetic rats

2005 ◽  
Vol 289 (5) ◽  
pp. H2234-H2243 ◽  
Author(s):  
Takayuki Matsumoto ◽  
Kentaro Wakabayashi ◽  
Tsuneo Kobayashi ◽  
Katsuo Kamata
Keyword(s):  
Mesenteric Artery ◽  
Diabetic Rats ◽  
Functional Change ◽  
Water Soluble ◽  
Mesenteric Arteries ◽  
Diabetic Rat ◽  
Adenylyl Cyclases ◽  
Camp Production ◽  
Functional Changes ◽  
Rat Mesenteric Artery

To assess the functional change in adenylyl cyclases (AC) associated with the diabetic state, we investigated AC-mediated relaxations and cAMP production in mesenteric arteries from rats with streptozotocin (STZ)-induced diabetes. The relaxations induced by the water-soluble forskolin (FSK) analog NKH477, which is a putative AC5 activator, but not by the β-adrenoceptor agonist isoproterenol (Iso) and the AC activator FSK, were reduced in intact diabetic mesenteric artery. In diabetic rats, however, Iso-, FSK-, and NKH477-induced relaxations were attenuated in the presence of inhibitors of nitric oxide synthase and cyclooxygenase. To exclude the influence of phosphodiesterase (PDE), we also examined the relaxations induced by several AC activators in the presence of 3-isobutyl-1-methylxanthine (IBMX; a PDE inhibitor). Under these conditions, the relaxation induced by Iso was greatly impaired in STZ-diabetic rats. This Iso-induced relaxation was significantly attenuated by pretreatment with SQ-22536, an AC inhibitor, in mesenteric rings from age-matched controls but not in those from STZ-diabetic rats. Under the same conditions, the relaxations induced by FSK or NKH477 were impaired in STZ-diabetic rats. Neither FSK- nor A-23187 (a Ca2+ ionophore)-induced cAMP production was significantly different between diabetics and controls. However, cAMP production induced by Iso or NKH477 was significantly impaired in diabetic mesenteric arteries. Expression of mRNAs and proteins for AC5/6 was lower in diabetic mesenteric arteries than in controls. These results suggest that AC-mediated relaxation is impaired in the STZ-diabetic rat mesenteric artery, perhaps reflecting a reduction in AC5/6 activity.

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