Phycoremediation potential of microalgae species for ethidium bromide removal from aqueous media

During the prodromal stages of sporulation in the Basidiomycete, Agaricus bisporus, mitochondria accumulate in the basidial cells, zygotes, in the gill tissues prior to entry of these mitochondria, together with two haploid nuclei and cytoplasmic ribosomes, into the exospores. The mitochondria contain prominent loci of DNA [Fig. 1]. A modified Kleinschmidt spread technique1 has been used to evaluate the DNA strands from purified whole mitochondria released by osmotic shock, mitochondrial DNA purified on CsCl gradients [density = 1.698 gms/cc], and DNA purified on ethidium bromide CsCl gradients. The DNA appeared as linear strands up to 25 u in length and circular forms 2.2-5.2 u in circumference. In specimens prepared by osmotic shock, many strands of DNA are apparently attached to membrane fragments [Fig. 2]. When mitochondria were ruptured in hypotonic sucrose and then fixed in glutaraldehyde, the ribosomes were released for electron microscopic examination.

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Deformation of Yeast Plasma Membrane by Ethidium Bromide

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100103334 ◽

1988 ◽

Vol 46 ◽

pp. 254-255

Author(s):

E. Keyhani

Keyword(s):

Plasma Membrane ◽

Thin Section ◽

Ethidium Bromide ◽

Freeze Fracture ◽

Mutagenic Effect ◽

Yeast Cells ◽

Hydrophobic Region ◽

Thin Section Electron Microscopy ◽

Section Electron ◽

Deep Pits

The mutagenic effect of ethidium bromide on the mitochondrial DNA is well established. Using thin section electron microscopy, it was shown that when yeast cells were grown in the presence of ethidium bromide, besides alterations in the mitochondria, the plasma membrane also showed alterations consisting of 75 to 110 nm-deep pits. Furthermore, ethidium bromide induced an increase in the length and number of endoplasmic reticulum and in the number of intracytoplasmic vesicles.Freeze-fracture, by splitting the hydrophobic region of the membrane, allows the visualization of the surface view of the membrane, and consequently, any alteration induced by ethidium bromide on the membrane can be better examined by this method than by the thin section method.Yeast cells, Candida utilis. were grown in the presence of 35 μM ethidium bromide. Cells were harvested and freeze-fractured according to the procedure previously described.

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Highly efficient and selective photocatalytic CO2 to CO conversion in aqueous solution

Chemical Communications ◽

10.1039/d0cc00879f ◽

2020 ◽

Vol 56 (27) ◽

pp. 3851-3854 ◽

Cited By ~ 6

Author(s):

Xiaomin Chai ◽

Hai-Hua Huang ◽

Huiping Liu ◽

Zhuofeng Ke ◽

Wen-Wen Yong ◽

...

Keyword(s):

Aqueous Solution ◽

Photocatalytic Performance ◽

Aqueous Media ◽

Highly Efficient ◽

Co Conversion

A Co-based complex displayed the highest photocatalytic performance for CO2 to CO conversion in aqueous media.

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A green and facile ultrasound-promoted synthesis of thioamide derivatives catalyzed by Cu(I)@Chitosan as a new bio-polymeric nano catalyst in aqueous media

10.3390/ecsoc-19-a028 ◽

2015 ◽

Author(s):

Mohammad.G Dekamin ◽

Niaz Kheirabi ◽

Zahra Karimi

Keyword(s):

Aqueous Media ◽

Nano Catalyst

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Extraction-Sorption Concentration of Resorcinol from Aqueous Media by Foamed Polyurethane

Химия в интересах устойчивого развития ◽

10.15372/khur20160111 ◽

2016 ◽

Vol 24 (1) ◽

Keyword(s):

Aqueous Media ◽

Sorption Concentration

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Detection of a covalent-ionic carbinolamine intermediate in aqueous media by SDTLC on silica gel plates

JPC - Journal of Planar Chromatography - Modern TLC ◽

10.1556/jpc.20.2007.3.11 ◽

2007 ◽

Vol 20 (3) ◽

pp. 227-230 ◽

Cited By ~ 1

Author(s):

Ana Reverdito ◽

Mariano García ◽

Alejandra Salerno ◽

Oscar Locani ◽

Isabel Perillo

Keyword(s):

Silica Gel ◽

Aqueous Media

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Temperature Dependence of the Self-Assembled Styrene Based Random Ionomers in Aqueous Solution

MRS Proceedings ◽

10.1557/proc-775-p6.27 ◽

2003 ◽

Vol 775 ◽

Author(s):

Sung-Hwa Oh ◽

Ju-Myung Song ◽

Joon-Seop Kim ◽

Hyang-Rim Oh ◽

Jeong-A Yu

Keyword(s):

Partition Coefficients ◽

Room Temperature ◽

Aqueous Media ◽

The Self ◽

Spectroscopic Methods ◽

Ion Content ◽

Repeat Units ◽

Notable Effect ◽

Order Of Magnitude ◽

Experimental Errors

AbstractSolution behaviors of poly(styrene-co-sodium methacrylate) were studied by fluorescence spectroscopic methods using pyrene as a probe. The mol% of methacrylate was in the range 3.6–9.4. Water and N,N-dimethylforamide(DMF) mixture was used as a solvent (DMF/water = 0.2 mol %). The critical micelle (or aggregation) concentrations of ionomers and the partition coefficients of pyrene were obtained the temperature range 10–80°C. At room temperature, the values of CMCs (or CACs) were in the range 4.7 ×10-6 5.3 ×10-6 g/mL and we could not find any notable effect of the content of ionic repeat units within the experimental errors. Unlike CMCs, as the ion content increased, partitioning of pyrene between the hydrophobic aggregates and an aqueous media decreased from 1.5 ×105 to 9.4 ×104. As the temperature increased from 10 to 80 °C, the values of CMCs increased less than one order of magnitude. While, the partition coefficients of pyrene decreased one order of magnitude and the effect of the ion content became negligible.

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