Enzymatic biodiesel production from crude Eruca sativa oil using Candida rugosa lipase in a solvent-free system using response surface methodology

Biofuels ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 93-99 ◽  
Author(s):  
Marzieh Aghababaie ◽  
Masoud Beheshti ◽  
Amir Razmjou ◽  
Abdol-Khalegh Bordbar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Candida Rugosa ◽  
Candida Rugosa Lipase ◽  
Solvent Free ◽  
Biodiesel Production ◽  
Eruca Sativa ◽  
Free System ◽  
Solvent Free System

scholarly journals Optimization of the production of biodiesel by a commercial immobilized lipase in a solvent-free system using a response surface methodology

2008 ◽  
Vol 73 (2) ◽  
pp. 147-156 ◽  
Author(s):  
Nevena Ognjanovic ◽  
Dejan Bezbradica ◽  
Zorica Knezevic
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Reaction Rate ◽  
Immobilized Lipase ◽  
Statistical Significance ◽  
Solvent Free ◽  
Biodiesel Production ◽  
Molar Ratio ◽  
Free System ◽  
Solvent Free System

Response surface methodology was used for the evaluation of the effects of various factors on the synthesis of biodiesel catalyzed with immobilized lipase from Rhizomucor miehei in a solvent-free system. The production of biodiesel was optimized and model response equations were obtained, enabling the prediction of biodiesel production from the values of the four main factors. It would seem that the reaction temperature and the amount of water predominantly determined the conversion process while the methanol/oil molar ratio had no significant influence on the reaction rate. The temperature and amount of water showed negative interactive effects on the observed reaction rate per amount of enzyme. However, there were no significant interactions among the other variables according to the test of statistical significance. The highest yield of 10.15 mol kg-1 enzyme was observed at 45?C with a 6:1 methanol to oil molar ratio and with no added water in the system.

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