Lipase-catalyzed transesterification of medium-long-medium structured lipid (MLM-SL) using palm olein and tricaprylin in packed-bed reactor (PBR)

Lipase-catalyzed transesterification between refined bleached deodorized palm olein (RBDO) and tricaprylin to produce medium-long-medium structured lipid (MLM-SL) in a packed bed reactor (PBR) has been investigated. A specific sn-1,3 commercial Lipozyme TL IM was used as biocatalyst. Within this study, the progress of transesterification was monitored especially for triacylglycerol (TAG) formation with equivalent carbon number (ECN) of 32, presumably 1,3-dicapryoyl-2-oleoyl-sn-glycerol (COC). Transesterification conditions investigated were residence times (i.e., 15, 30, and 60 min) and enzyme loadings (2.0 and 4.5 g). The highest yield of ECN 32 (13%) and transesterification degree (71%) were obtained at residence time of 15 mins for both enzyme loadings. Longer residence time seemed to facilitate lipid hydrolysis over transesterification. This was indicated by the number of peaks appearing in the high-performance liquid chromatography (HPLC) chromatograms and the reduction of fat slip melting point (SMP). Additionally, the highest productivity was obtained at 2.0 g enzyme loading. Conclusively, this study has demonstrated the potential use of packed-bed reactor with immobilized Lipozyme TL IM for continuous synthesis of MLM-SLs especially TAG with ECN32.

An Insight to Nanostructured Lipid Carrier System

In pharmaceutical field, many drugs are being invented to combat the existing new diseases. The winds of change in the drug scenario are blowing forcefully worldwide. The emergence of new technologies provides an unique opportunities to exploit novel approaches in drug delivery. A shift from conventional drug delivery to novel drug delivery is noticed as shift from conventional drug delivery suffers from various drawbacks, But these new mighty compounds in drug therapy solely are not sufficient to meet the today’s need. There is an urgent need for the smart technology, as the drugs available suffer from serious problems like poor solubility and poor bioavailability. Most of the drugs that are available therapeutically comes under BCS class II ie, poorly soluble and high lipophilc. To assure progress in drug therapy, the development of new drugs merely is not sufficient. Issues arising in delivery of new drugs should also be addressed. Thus there is an emergent need to improve the bioavailability of these drugs, the only remedy or boon for such drugs is the discovery of some smart technologies which can improve the bioavailability of these drugs. Nano drug delivery systems are the one such universal approach which fulfills the lacuna, which exists in conventional drug delivery systems. These Nano drug delivery systems, improves the pharmacokinetic profiles of many drugs. In 1980 K. Eric Drexler developed and popularized the concept of nanotechnology. In this review a deep insight on Nano structured lipid carriers is discussed elaborating its birth, significant qualities compared to other colloidal systems, its structure, characteristics, preparation and application are spotted. Key words: BCS class, Lipophilic, Nano technology, Nano structured lipid carrier.