PCR fingerprinting: a convenient molecular tool to distinguish betweenCandida dubliniensisandCandida albicans

2001 ◽  
Vol 39 (2) ◽  
pp. 185-193 ◽  
Author(s):  
W. Meyer ◽  
K. Maszewska ◽  
T. C. Sorrell
Keyword(s):  
Molecular Tool ◽  
Pcr Fingerprinting

scholarly journals Strain Typing of Trichosporon asahii Clinical Isolates by Random Amplification of Polymorphic DNA (RAPD) Analysis

2021 ◽  
Author(s):  
Thayanidhi Premamalini ◽  
Vijayaraman Rajyoganandh ◽  
Ramaraj Vijayakumar ◽  
Hemanth Veena ◽  
Anupma Jyoti Kindo ◽  
...  
Keyword(s):  
Genetic Relatedness ◽  
Rapd Analysis ◽  
Clinical Isolates ◽  
Clinical Samples ◽  
Strain Typing ◽  
Trichosporon Asahii ◽  
Pcr Fingerprinting ◽  
Specific Pcr ◽  
Rapd Primer ◽  
Random Amplification

Abstract Objective The aim of this study was to identify and isolate Trichosporon asahii (T. asahii) from clinical samples and to assess the genetic relatedness of the most frequently isolated strains of T. asahii using random amplification of polymorphic DNA (RAPD) primers GAC-1 and M13. Methods All the clinical samples that grew Trichosporon species, identified and confirmed by polymerase chain reaction (PCR) using Trichosporon genus-specific primers, were considered for the study. Confirmation of the species T. asahii was carried out by T. asahii-specific PCR. Fingerprinting of the most frequently isolated T. asahii isolates was carried out by RAPD using random primers GAC-1 and M13. Results Among the 72 clinical isolates of Trichosporon sp. confirmed by Trichosporon-specific PCR, 65 were found to be T. asahii as identified by T. asahii-specific PCR. Fingerprinting of the 65 isolates confirmed as T. asahii using GAC-1 RAPD primer yielded 11 different patterns, whereas that of M13 primer produced only 5 patterns. The pattern I was found to be the most predominant type (29.2%) followed by pattern III (16.9%) by GAC-1 primer. Conclusions This study being the first of its kind in India on strain typing of T. asahii isolates by adopting RAPD analysis throws light on genetic diversity among the T. asahii isolates from clinical samples. Fingerprinting by RAPD primer GAC-1 identified more heterogeneity among the T. asahii isolates than M13.

scholarly journals Genetic relationship in the ‘Bacillus cereusgroup’ by rep-PCR fingerprinting and sequencing of aBacillus anthracis-specific rep-PCR fragment

2003 ◽  
Vol 94 (6) ◽  
pp. 1108-1119 ◽  
Author(s):  
A. Cherif ◽  
L. Brusetti ◽  
S. Borin ◽  
A. Rizzi ◽  
A. Boudabous ◽  
...  
Keyword(s):  
Genetic Relationship ◽  
Pcr Fingerprinting

scholarly journals Detection of influenza C during an outbreak at an internal school, using a molecular tool; Havana, Cuba, September 2006

2008 ◽  
Vol 12 (6) ◽  
pp. e129-e130 ◽  
Author(s):  
Alexander Piñón Ramos ◽  
Belsy Acosta Herrera ◽  
Odalys Valdés Ramírez ◽  
Clara Savón Valdés ◽  
Angel Goyenechea Hernández ◽  
...  
Keyword(s):  
Molecular Tool

PCR fingerprinting for epidemiological studies of Staphylococcus aureus

1994 ◽  
Vol 20 (4) ◽  
pp. 235-247 ◽  
Author(s):  
Alex van Belkum ◽  
René Bax ◽  
Piet J.C. van der Straaten ◽  
Wim G.V. Quint ◽  
Etel Veringa
Keyword(s):  
Staphylococcus Aureus ◽  
Epidemiological Studies ◽  
Pcr Fingerprinting

scholarly journals Selection of enhanced antimicrobial activity posing lactic acid bacteria characterised by (GTG)5-PCR fingerprinting

2014 ◽  
Vol 52 (7) ◽  
pp. 4124-4134 ◽  
Author(s):  
Joana Šalomskienė ◽  
Asta Abraitienė ◽  
Dovilė Jonkuvienė ◽  
Irena Mačionienė ◽  
Jūratė Repečkienė
Keyword(s):  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pcr Fingerprinting ◽  
Selection Of

scholarly journals Genotyping, serotyping and determination of mating-type of Cryptococcus neoformans clinical isolates from São Paulo State, Brazil

2007 ◽  
Vol 49 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Marcelo Teruyuki Matsumoto ◽  
Ana Marisa Fusco-Almeida ◽  
Lilian Cristiane Baeza ◽  
Márcia de Souza Carvalho Melhem ◽  
Maria José Soares Medes-Giannini
Keyword(s):  
Cryptococcus Neoformans ◽  
Mating Type ◽  
Rflp Analysis ◽  
Sao Paulo ◽  
Clinical Isolates ◽  
São Paulo ◽  
Paulo State ◽  
Pcr Fingerprinting ◽  
Pcr Rflp ◽  
São Paulo State

The basidiomycetous yeast Cryptococcus neoformans is an important fungal pathogen mainly in immunocompromised patients. In this study, 47 clinical isolates of C. neoformans from regions of São Paulo State were studied serologically by using the Crypto Check Iatron RM 304-K kit, their genetic diversity was estimated by PCR-fingerprinting with a microsatellite-specific sequence (GACA)4, RAPD with primer 6 (Amersham Pharmacia Biotech), PCR-restriction fragment length polymorphism (RFLP) analysis of the phospholipase B gene (PLB1) digested with AvaI and mating type analysis by PCR. All 47 strains isolated from HIV positive patients included in this study were serotype A and MATalpha. The majority of the isolates (45/47) were VNI and only two were VNII by PCR-fingerprinting and PCR-RFLP analysis. High degree of homogeneity was observed when (GACA)4 was used, being highly correlated (> 0.9). In contrast, the RAPD analysis was more heterogeneous with higher number of molecular profiles. By PCR-RFLP, no new molecular type was found, enhancing the suggestion that the differences based on conserved gene as PLB1, can be resultant of ongoing divergent evolution within the C. neoformans complex, into the current eight subtypes. Our results furnish new information on the molecular epidemiology of C. neoformans in the southeast region of Brazil.

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